Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells

Due to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processe...

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Autores: Polo Montalvo, Alberto, Casarrubios Molina, Laura, Serrano, María Concepción, Sanvicente, Adrián, Feito Castellano, María José, Arcos Navarrete, Daniel, Portolés Pérez, María Teresa
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/4854
Acesso em linha:https://hdl.handle.net/20.500.14352/4854
Access Level:acceso abierto
Palavra-chave:615.46
546
Mesoporous bioactive glass
Osteogenesis
Angiogenesis
Endothelial progenitor cells
Pre-osteoblasts
Macrophages
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
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oai_identifier_str oai:docta.ucm.es:20.500.14352/4854
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network_name_str España
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spelling Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor CellsPolo Montalvo, AlbertoCasarrubios Molina, LauraSerrano, María ConcepciónSanvicente, AdriánFeito Castellano, María JoséArcos Navarrete, DanielPortolés Pérez, María Teresa615.46546Mesoporous bioactive glassOsteogenesisAngiogenesisEndothelial progenitor cellsPre-osteoblastsMacrophagesMaterialesQuímica inorgánica (Farmacia)3312 Tecnología de MaterialesDue to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processes, depending on their phenotype. In this work, the action of ion release from MBG-75S, with a molar composition of 75SiO2-20CaO-5P2O5, on osteogenesis and angiogenesis and the modulatory role of macrophages have been assessed in vitro with MC3T3-E1 pre-osteoblasts and endothelial progenitor cells (EPCs) in monoculture and in coculture with RAW 264.7 macrophages. Ca2+, phosphorous, and silicon ions released from MBG-75S were measured in the culture medium during both differentiation processes. Alkaline phosphatase activity and matrix mineralization were quantified as the key markers of osteogenic differentiation in MC3T3-E1 cells. The expression of CD31, CD34, VEGFR2, eNOS, and vWF was evaluated to characterize the EPC differentiation into mature endothelial cells. Other cellular parameters analyzed included the cell size and complexity, intracellular calcium, and intracellular content of the reactive oxygen species. The results obtained indicate that the ions released by MBG-75S promote osteogenesis and angiogenesis in vitro, evidencing a macrophage inhibitory role in these processes and demonstrating the high potential of MBG-75S for the preparation of implants for bone regeneration.MDPIUniversidad Complutense de Madrid20212021-07-2720212021-07-27journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/4854reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/48542026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
title Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
spellingShingle Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
Polo Montalvo, Alberto
615.46
546
Mesoporous bioactive glass
Osteogenesis
Angiogenesis
Endothelial progenitor cells
Pre-osteoblasts
Macrophages
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
title_short Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
title_full Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
title_fullStr Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
title_full_unstemmed Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
title_sort Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
dc.creator.none.fl_str_mv Polo Montalvo, Alberto
Casarrubios Molina, Laura
Serrano, María Concepción
Sanvicente, Adrián
Feito Castellano, María José
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author Polo Montalvo, Alberto
author_facet Polo Montalvo, Alberto
Casarrubios Molina, Laura
Serrano, María Concepción
Sanvicente, Adrián
Feito Castellano, María José
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author_role author
author2 Casarrubios Molina, Laura
Serrano, María Concepción
Sanvicente, Adrián
Feito Castellano, María José
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.46
546
Mesoporous bioactive glass
Osteogenesis
Angiogenesis
Endothelial progenitor cells
Pre-osteoblasts
Macrophages
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
topic 615.46
546
Mesoporous bioactive glass
Osteogenesis
Angiogenesis
Endothelial progenitor cells
Pre-osteoblasts
Macrophages
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
description Due to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processes, depending on their phenotype. In this work, the action of ion release from MBG-75S, with a molar composition of 75SiO2-20CaO-5P2O5, on osteogenesis and angiogenesis and the modulatory role of macrophages have been assessed in vitro with MC3T3-E1 pre-osteoblasts and endothelial progenitor cells (EPCs) in monoculture and in coculture with RAW 264.7 macrophages. Ca2+, phosphorous, and silicon ions released from MBG-75S were measured in the culture medium during both differentiation processes. Alkaline phosphatase activity and matrix mineralization were quantified as the key markers of osteogenic differentiation in MC3T3-E1 cells. The expression of CD31, CD34, VEGFR2, eNOS, and vWF was evaluated to characterize the EPC differentiation into mature endothelial cells. Other cellular parameters analyzed included the cell size and complexity, intracellular calcium, and intracellular content of the reactive oxygen species. The results obtained indicate that the ions released by MBG-75S promote osteogenesis and angiogenesis in vitro, evidencing a macrophage inhibitory role in these processes and demonstrating the high potential of MBG-75S for the preparation of implants for bone regeneration.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-27
2021
2021-07-27
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/4854
url https://hdl.handle.net/20.500.14352/4854
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411620905549824
score 15,300719

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