Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala

In mammals, the central extended amygdala is critical for the regulation of the stress response. This regulation is extremely complex, involving multiple subpopulations of GABAergic neurons and complex networks of internal and external connections. Two neuron subpopulations expressing corticotropin-...

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Detalles Bibliográficos
Autores: Pross, Alessandra, Hanafi-Metwalli, Alek, Abellán Ródenas, Antonio, Desfilis, Ester, Medina Hernández, Loreta Mª
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/465163
Acceso en línea:https://doi.org/10.1002/cne.25569
https://hdl.handle.net/10459.1/465163
Access Level:acceso abierto
Palabra clave:BSTL
CRF
CRFR2
Islet1
Pax6
RRID:AB_2313674
RRID:AB_2534069
RRID:AB_2890548
RRID:AB_514500
RRID:AB_528315
RRID:AB_528427
RRID:AB_732415
Anxiety circuits
Central
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spelling Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdalaPross, AlessandraHanafi-Metwalli, AlekAbellán Ródenas, AntonioDesfilis, EsterMedina Hernández, Loreta MªBSTLCRFCRFR2Islet1Pax6RRID:AB_2313674RRID:AB_2534069RRID:AB_2890548RRID:AB_514500RRID:AB_528315RRID:AB_528427RRID:AB_732415Anxiety circuitsCentralIn mammals, the central extended amygdala is critical for the regulation of the stress response. This regulation is extremely complex, involving multiple subpopulations of GABAergic neurons and complex networks of internal and external connections. Two neuron subpopulations expressing corticotropin-releasing factor (CRF), located in the central amygdala and the lateral bed nucleus of the stria terminalis (BSTL), play a key role in the long-term component of fear learning and in sustained fear responses akin to anxiety. Very little is known about the regulation of stress by the amygdala in nonmammals, hindering efforts for trying to improve animal welfare. In birds, one of the major problems relates to the high evolutionary divergence of the telencephalon, where the amygdala is located. In the present study, we aimed to investigate the presence of CRF neurons of the central extended amygdala in chicken and the local connections within this region. We found two major subpopulations of CRF cells in BSTL and the medial capsular central amygdala of chicken. Based on multiple labeling of CRF mRNA with different developmental transcription factors, all CRF neurons seem to originate within the telencephalon since they express Foxg1, and there are two subtypes with different embryonic origins that express Islet1 or Pax6. In addition, we demonstrated direct projections from Pax6 cells of the capsular central amygdala to BSTL and the oval central amygdala. We also found projections from Islet1 cells of the oval central amygdala to BSTL, which may constitute an indirect pathway for the regulation of BSTL output cells. Part of these projections may be mediated by CRF cells, in agreement with the expression of CRF receptors in both Ceov and BSTL. Our results show a complex organization of the central extended amygdala in chicken and open new venues for studying how different cells and circuits regulate stress in these animals.European Union’s Horizon 2020, Grant/Award Number:H2020-MSCA-ITN-2018-812777; Agencia Estatal de Investigación, Grant/Award Number:PID2019-108725RB-100Wiley2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1002/cne.25569https://hdl.handle.net/10459.1/465163reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglés108725RBReproducció del document publicat a: https://doi.org/10.1002/cne.25569Journal of Comparative Neurology, 2024, vol. 532, núm. 2info:eu-repo/grantAgreement/EC/H2020/812777Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/oai:repositori.udl.cat:10459.1/4651632026-06-24T12:42:17Z
dc.title.none.fl_str_mv Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
title Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
spellingShingle Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
Pross, Alessandra
BSTL
CRF
CRFR2
Islet1
Pax6
RRID:AB_2313674
RRID:AB_2534069
RRID:AB_2890548
RRID:AB_514500
RRID:AB_528315
RRID:AB_528427
RRID:AB_732415
Anxiety circuits
Central
title_short Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
title_full Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
title_fullStr Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
title_full_unstemmed Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
title_sort Subpopulations of corticotropin-releasing factor containing neurons and internal circuits in the chicken central extended amygdala
dc.creator.none.fl_str_mv Pross, Alessandra
Hanafi-Metwalli, Alek
Abellán Ródenas, Antonio
Desfilis, Ester
Medina Hernández, Loreta Mª
author Pross, Alessandra
author_facet Pross, Alessandra
Hanafi-Metwalli, Alek
Abellán Ródenas, Antonio
Desfilis, Ester
Medina Hernández, Loreta Mª
author_role author
author2 Hanafi-Metwalli, Alek
Abellán Ródenas, Antonio
Desfilis, Ester
Medina Hernández, Loreta Mª
author2_role author
author
author
author
dc.subject.none.fl_str_mv BSTL
CRF
CRFR2
Islet1
Pax6
RRID:AB_2313674
RRID:AB_2534069
RRID:AB_2890548
RRID:AB_514500
RRID:AB_528315
RRID:AB_528427
RRID:AB_732415
Anxiety circuits
Central
topic BSTL
CRF
CRFR2
Islet1
Pax6
RRID:AB_2313674
RRID:AB_2534069
RRID:AB_2890548
RRID:AB_514500
RRID:AB_528315
RRID:AB_528427
RRID:AB_732415
Anxiety circuits
Central
description In mammals, the central extended amygdala is critical for the regulation of the stress response. This regulation is extremely complex, involving multiple subpopulations of GABAergic neurons and complex networks of internal and external connections. Two neuron subpopulations expressing corticotropin-releasing factor (CRF), located in the central amygdala and the lateral bed nucleus of the stria terminalis (BSTL), play a key role in the long-term component of fear learning and in sustained fear responses akin to anxiety. Very little is known about the regulation of stress by the amygdala in nonmammals, hindering efforts for trying to improve animal welfare. In birds, one of the major problems relates to the high evolutionary divergence of the telencephalon, where the amygdala is located. In the present study, we aimed to investigate the presence of CRF neurons of the central extended amygdala in chicken and the local connections within this region. We found two major subpopulations of CRF cells in BSTL and the medial capsular central amygdala of chicken. Based on multiple labeling of CRF mRNA with different developmental transcription factors, all CRF neurons seem to originate within the telencephalon since they express Foxg1, and there are two subtypes with different embryonic origins that express Islet1 or Pax6. In addition, we demonstrated direct projections from Pax6 cells of the capsular central amygdala to BSTL and the oval central amygdala. We also found projections from Islet1 cells of the oval central amygdala to BSTL, which may constitute an indirect pathway for the regulation of BSTL output cells. Part of these projections may be mediated by CRF cells, in agreement with the expression of CRF receptors in both Ceov and BSTL. Our results show a complex organization of the central extended amygdala in chicken and open new venues for studying how different cells and circuits regulate stress in these animals.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1002/cne.25569
https://hdl.handle.net/10459.1/465163
url https://doi.org/10.1002/cne.25569
https://hdl.handle.net/10459.1/465163
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 108725RB
Reproducció del document publicat a: https://doi.org/10.1002/cne.25569
Journal of Comparative Neurology, 2024, vol. 532, núm. 2
info:eu-repo/grantAgreement/EC/H2020/812777
dc.rights.none.fl_str_mv Attribution-NonCommercial 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411618212806656
score 15.81155

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