Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections

Pseudomonas aeruginosa is a relevant pathogen in chronic respiratory infections, which are usually associated with biofilm formation, complicating in vitro modeling and effective treatment strategies. While P. aeruginosa can coexist with several microorganisms, its association with Staphylococcus au...

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Detalles Bibliográficos
Autores: Admella, Joana, Alcàcer Almansa, Júlia, Julian, Esther, Torrents Serra, Eduard
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/223701
Acceso en línea:https://hdl.handle.net/2445/223701
Access Level:acceso abierto
Palabra clave:Staphylococcus aureus
Cèl·lules epitelials
Epithelial cells
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spelling Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfectionsAdmella, JoanaAlcàcer Almansa, JúliaJulian, EstherTorrents Serra, EduardStaphylococcus aureusCèl·lules epitelialsStaphylococcus aureusEpithelial cellsPseudomonas aeruginosa is a relevant pathogen in chronic respiratory infections, which are usually associated with biofilm formation, complicating in vitro modeling and effective treatment strategies. While P. aeruginosa can coexist with several microorganisms, its association with Staphylococcus aureus is widespread in cystic fibrosis (CF) patients and other bronchiectasis. Finding a reliable and straightforward in vitro model to study long-term P. aeruginosa infections is extremely hard due to the secretion of highly virulent toxins that compromise the model within less than 10 h. Several optimizations, including the use of bovine serum albumin (BSA) and extracellular matrix proteins, led to enhanced A549 cell viability up to 30 h post-infection. Within this time frame, we developed P. aeruginosa biofilms, explored host-pathogen interactions, and delved deeper into the relationship between P. aeruginosa and S. aureus. Additionally, ciprofloxacin treatment was evaluated, revealing changes and differences in antibiotic susceptibility and underlying significant differences between bacterial strainsElsevier2025202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfhttps://hdl.handle.net/2445/223701Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproduccoió del document publicat a: https://doi.org/10.1016/j.isci.2025.113620Iscience, 2025, vol. 28, num. 11, 113620https://doi.org/10.1016/j.isci.2025.113620cc-by-nc-nd (c) Admella et al., 2025http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2237012026-05-29T05:05:01Z
dc.title.none.fl_str_mv Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
title Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
spellingShingle Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
Admella, Joana
Staphylococcus aureus
Cèl·lules epitelials
Staphylococcus aureus
Epithelial cells
title_short Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
title_full Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
title_fullStr Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
title_full_unstemmed Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
title_sort Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
dc.creator.none.fl_str_mv Admella, Joana
Alcàcer Almansa, Júlia
Julian, Esther
Torrents Serra, Eduard
author Admella, Joana
author_facet Admella, Joana
Alcàcer Almansa, Júlia
Julian, Esther
Torrents Serra, Eduard
author_role author
author2 Alcàcer Almansa, Júlia
Julian, Esther
Torrents Serra, Eduard
author2_role author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Cèl·lules epitelials
Staphylococcus aureus
Epithelial cells
topic Staphylococcus aureus
Cèl·lules epitelials
Staphylococcus aureus
Epithelial cells
description Pseudomonas aeruginosa is a relevant pathogen in chronic respiratory infections, which are usually associated with biofilm formation, complicating in vitro modeling and effective treatment strategies. While P. aeruginosa can coexist with several microorganisms, its association with Staphylococcus aureus is widespread in cystic fibrosis (CF) patients and other bronchiectasis. Finding a reliable and straightforward in vitro model to study long-term P. aeruginosa infections is extremely hard due to the secretion of highly virulent toxins that compromise the model within less than 10 h. Several optimizations, including the use of bovine serum albumin (BSA) and extracellular matrix proteins, led to enhanced A549 cell viability up to 30 h post-infection. Within this time frame, we developed P. aeruginosa biofilms, explored host-pathogen interactions, and delved deeper into the relationship between P. aeruginosa and S. aureus. Additionally, ciprofloxacin treatment was evaluated, revealing changes and differences in antibiotic susceptibility and underlying significant differences between bacterial strains
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/223701
url https://hdl.handle.net/2445/223701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproduccoió del document publicat a: https://doi.org/10.1016/j.isci.2025.113620
Iscience, 2025, vol. 28, num. 11, 113620
https://doi.org/10.1016/j.isci.2025.113620
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Admella et al., 2025
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Admella et al., 2025
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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