Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression

This article belongs to the Special Issue Targeting TRP Channels for Pain, Itch and Inflammation Relief.

Bibliographic Details
Authors: Hernández-Ortego, Pablo, Torres-Montero, Remedios, Peña, Elvira de la, Viana, Félix, Fernández-Trillo, Jorge
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304491
Online Access:http://hdl.handle.net/10261/304491
https://api.elsevier.com/content/abstract/scopus_id/85144557223
Access Level:Open access
Keyword:TRPM8
Western blot
Immunocytochemistry
Immunohistochemistry
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
id ES_7cc8a628d95f706d838d3c02aeea34f8
oai_identifier_str oai:digital.csic.es:10261/304491
network_acronym_str ES
network_name_str España
repository_id_str
spelling Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expressionHernández-Ortego, PabloTorres-Montero, RemediosPeña, Elvira de laViana, FélixFernández-Trillo, JorgeTRPM8Western blotImmunocytochemistryImmunohistochemistryhttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesThis article belongs to the Special Issue Targeting TRP Channels for Pain, Itch and Inflammation Relief.TRPM8 is a non-selective cation channel expressed in primary sensory neurons and other tissues, including the prostate and urothelium. Its participation in different physiological and pathological processes such as thermoregulation, pain, itch, inflammation and cancer has been widely described, making it a promising target for therapeutic approaches. The detection and quantification of TRPM8 seems crucial for advancing the knowledge of the mechanisms underlying its role in these pathophysiological conditions. Antibody-based techniques are commonly used for protein detection and quantification, although their performance with many ion channels, including TRPM8, is suboptimal. Thus, the search for reliable antibodies is of utmost importance. In this study, we characterized the performance of six TRPM8 commercial antibodies in three immunodetection techniques: Western blot, immunocytochemistry and immunohistochemistry. Different outcomes were obtained for the tested antibodies; two of them proved to be successful in detecting TRPM8 in the three approaches while, in the conditions tested, the other four were acceptable only for specific techniques. Considering our results, we offer some insight into the usefulness of these antibodies for the detection of TRPM8 depending on the methodology of choice.During the course of this work, PH was supported by an FPI predoctoral fellowship (BES-2017-080782). This study was funded by MICIN/AEI/10.13039/501100011033 (PID2019-108194RB-I00), Generalitat Valenciana (PROMETEO/2021/031) and the Severo Ochoa Programme for Centres of Excellence in R&D (ref. SEV-2017-0723).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Generalitat ValencianaViana, Félix [0000-0003-1439-949X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/304491https://api.elsevier.com/content/abstract/scopus_id/85144557223reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//BES-2017-080782info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108194RB-I00info:eu-repo/grantAgreement/AEI//SEV-2017-0723International journal of molecular sciencesThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms232416164https://doi.org/10.3390/ijms232416164Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3044912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
title Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
spellingShingle Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
Hernández-Ortego, Pablo
TRPM8
Western blot
Immunocytochemistry
Immunohistochemistry
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
title_full Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
title_fullStr Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
title_full_unstemmed Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
title_sort Validation of six commercial antibodies for the detection of heterologous and endogenous TRPM8 ion channel expression
dc.creator.none.fl_str_mv Hernández-Ortego, Pablo
Torres-Montero, Remedios
Peña, Elvira de la
Viana, Félix
Fernández-Trillo, Jorge
author Hernández-Ortego, Pablo
author_facet Hernández-Ortego, Pablo
Torres-Montero, Remedios
Peña, Elvira de la
Viana, Félix
Fernández-Trillo, Jorge
author_role author
author2 Torres-Montero, Remedios
Peña, Elvira de la
Viana, Félix
Fernández-Trillo, Jorge
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Generalitat Valenciana
Viana, Félix [0000-0003-1439-949X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv TRPM8
Western blot
Immunocytochemistry
Immunohistochemistry
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic TRPM8
Western blot
Immunocytochemistry
Immunohistochemistry
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description This article belongs to the Special Issue Targeting TRP Channels for Pain, Itch and Inflammation Relief.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304491
https://api.elsevier.com/content/abstract/scopus_id/85144557223
url http://hdl.handle.net/10261/304491
https://api.elsevier.com/content/abstract/scopus_id/85144557223
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//BES-2017-080782
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108194RB-I00
info:eu-repo/grantAgreement/AEI//SEV-2017-0723
International journal of molecular sciences
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms232416164
https://doi.org/10.3390/ijms232416164
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411615840927744
score 15,811543

Similar Items