Pathogenic potential of Saccharomyces strains isolated from dietary supplements.

Saccharomyces cerevisiae plays a beneficial role in health because of its intrinsic nutritional value and bio-functional properties, which is why it is also used as a dietary supplement. However, the perception that S. cerevisiae is harmless has changed due to an increasing number of infections caus...

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Autores: Llopis, Silvia, Hernández Haro, Carolina, Monteoliva Díaz, Lucía, Querol, Amparo, Molina, María, Fernández Espinar, María T.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/34917
Acceso en línea:https://hdl.handle.net/20.500.14352/34917
Access Level:acceso abierto
Palabra clave:579
Saccharomyces cerevisiae
Dietary Supplements
yeasts isolated
Microbiología (Farmacia)
3302.03 Microbiología Industrial
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repository_id_str
spelling Pathogenic potential of Saccharomyces strains isolated from dietary supplements.Llopis, SilviaHernández Haro, CarolinaMonteoliva Díaz, LucíaQuerol, AmparoMolina, MaríaFernández Espinar, María T.579Saccharomyces cerevisiaeDietary Supplementsyeasts isolatedMicrobiología (Farmacia)3302.03 Microbiología IndustrialSaccharomyces cerevisiae plays a beneficial role in health because of its intrinsic nutritional value and bio-functional properties, which is why it is also used as a dietary supplement. However, the perception that S. cerevisiae is harmless has changed due to an increasing number of infections caused by this yeast. Given this scenario, we have tested whether viable strains contained in dietary supplements displayed virulence-associated phenotypic traits that could contribute to virulence in humans. We have also performed an in vivo study of the pathogenic potential of these strains using a murine model of systemic infection by intravenous inoculation. A total of 5 strains were isolated from 22 commercial products and tested. Results highlight one strain (D14) in terms of burden levels in brains and kidneys and ability to cause death, whereas the other two strains (D2 and D4) were considered of low virulence. Our results suggest a strong relationship between some of the virulence-associated phenotypic traits (ability to grow at 39°C and pseudohyphal growth) and the in vivo virulence in a mouse model of intravenous inoculation for isolates under study. The isolate displaying greatest virulence (D14) was evaluated in an experimental murine model of gastrointestinal infection with immunosuppression and disruption of mucosal integrity, which are common risk factors for developing infection in humans, and results were compared with an avirulent strain (D23). We showed that D14 was able to spread to mesenteric nodes and distant organs under these conditions. Given the widespread consumption of dietary supplements, we recommend only safe strains be used.Public Library of ScienceUniversidad Complutense de Madrid20142014-05-3020142014-05-30journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/34917reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/349172026-06-02T12:44:21Z
dc.title.none.fl_str_mv Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
title Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
spellingShingle Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
Llopis, Silvia
579
Saccharomyces cerevisiae
Dietary Supplements
yeasts isolated
Microbiología (Farmacia)
3302.03 Microbiología Industrial
title_short Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
title_full Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
title_fullStr Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
title_full_unstemmed Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
title_sort Pathogenic potential of Saccharomyces strains isolated from dietary supplements.
dc.creator.none.fl_str_mv Llopis, Silvia
Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Querol, Amparo
Molina, María
Fernández Espinar, María T.
author Llopis, Silvia
author_facet Llopis, Silvia
Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Querol, Amparo
Molina, María
Fernández Espinar, María T.
author_role author
author2 Hernández Haro, Carolina
Monteoliva Díaz, Lucía
Querol, Amparo
Molina, María
Fernández Espinar, María T.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
Saccharomyces cerevisiae
Dietary Supplements
yeasts isolated
Microbiología (Farmacia)
3302.03 Microbiología Industrial
topic 579
Saccharomyces cerevisiae
Dietary Supplements
yeasts isolated
Microbiología (Farmacia)
3302.03 Microbiología Industrial
description Saccharomyces cerevisiae plays a beneficial role in health because of its intrinsic nutritional value and bio-functional properties, which is why it is also used as a dietary supplement. However, the perception that S. cerevisiae is harmless has changed due to an increasing number of infections caused by this yeast. Given this scenario, we have tested whether viable strains contained in dietary supplements displayed virulence-associated phenotypic traits that could contribute to virulence in humans. We have also performed an in vivo study of the pathogenic potential of these strains using a murine model of systemic infection by intravenous inoculation. A total of 5 strains were isolated from 22 commercial products and tested. Results highlight one strain (D14) in terms of burden levels in brains and kidneys and ability to cause death, whereas the other two strains (D2 and D4) were considered of low virulence. Our results suggest a strong relationship between some of the virulence-associated phenotypic traits (ability to grow at 39°C and pseudohyphal growth) and the in vivo virulence in a mouse model of intravenous inoculation for isolates under study. The isolate displaying greatest virulence (D14) was evaluated in an experimental murine model of gastrointestinal infection with immunosuppression and disruption of mucosal integrity, which are common risk factors for developing infection in humans, and results were compared with an avirulent strain (D23). We showed that D14 was able to spread to mesenteric nodes and distant organs under these conditions. Given the widespread consumption of dietary supplements, we recommend only safe strains be used.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-05-30
2014
2014-05-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/34917
url https://hdl.handle.net/20.500.14352/34917
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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