A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status

The information provided by SARS-CoV-2 spike (S)-targeting immunoassays can be instrumental in clinical-decision making. We compared the performance of the Elecsys (R) Anti-SARS-CoV-2 S assay (Roche Diagnostics) and the LIAISON (R) SARS-CoV-2 TrimericS IgG assay (DiaSorin) using a total of 1176 sera...

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Authors: Camacho, Jorge, Albert, Eliseo, Zulaica, Joao, Alvarez-Rodriguez, Beatriz, Rusu, Luciana, Olea, Beatriz, Alcaraz, Maria Jesus, Geller, Ron, Gimenez, Estela, Navarro, David
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:INCLIVA
Repository:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p17040
Online Access:https://incliva.portalinvestigacion.com/publicaciones/17040
Access Level:Open access
Keyword:antibodies
DiaSorin immunoassay
neutralizing antibodies
receptor binding domain antibodies
Roche immunoassay
SARS-CoV-2
S-trimeric antibodies
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spelling A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections statusCamacho, JorgeAlbert, EliseoZulaica, JoaoAlvarez-Rodriguez, BeatrizRusu, LucianaOlea, BeatrizAlcaraz, Maria JesusGeller, RonGimenez, EstelaNavarro, DavidantibodiesDiaSorin immunoassayneutralizing antibodiesreceptor binding domain antibodiesRoche immunoassaySARS-CoV-2S-trimeric antibodiesThe information provided by SARS-CoV-2 spike (S)-targeting immunoassays can be instrumental in clinical-decision making. We compared the performance of the Elecsys (R) Anti-SARS-CoV-2 S assay (Roche Diagnostics) and the LIAISON (R) SARS-CoV-2 TrimericS IgG assay (DiaSorin) using a total of 1176 sera from 797 individuals, of which 286 were from vaccinated-SARS-CoV-2/experienced (Vac-Ex), 581 from vaccinated/naive (Vac-N), 147 from unvaccinated/experienced (Unvac-Ex), and 162 from unvaccinated/naive (Unvac-N) individuals. The Roche assay returned a higher number of positive results (907 vs. 790; p = 0.45; overall sensitivity: 89.3% vs. 77.6%). The concordance between results provided by the two immunoassays was higher for sera from Vac-N (kappa: 0.58; interquartile ranges [IQR]: 0.50-0.65) than for sera from Vac-Ex (kappa: 0.19; IQR: -0.14 to 0.52) or Unvac-Ex (kappa: 0.18; IQR: 0.06-0.30). Discordant results occurred more frequently among sera from Unvac-Ex (34.7%) followed by Vac-N (14.6%) and Vac-Ex (2.7%). Antibody levels quantified by both immunoassays were not significantly different when <250 (p = 0.87) or <1000BAU/ml (p = 0.13); in contrast, for sera >= 1000BAU/ml, the Roche assay returned significantly higher values than the DiaSorin assay (p < 0.008). Neutralizing antibody titers (NtAb) were measured in 127 sera from Vac-Ex or Vac-N using a S-pseudotyped virus neutralization assay of Wuhan-Hu-1, Omicron BA.1, and Omicron BA.2. The correlation between antibody levels and NtAb titers was higher for sera from Vac-N than those from Vac-Ex, irrespective of the (sub)variant considered. In conclusion, neither qualitative nor quantitative results returned by both immunoassays are interchangeable. The performance of both assays was found to be greatly influenced by the vaccination and SARS-CoV-2 infection status of individuals.WILEY2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/17040JOURNAL OF MEDICAL VIROLOGYISSN: 01466615ISSNe: 10969071reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p170402026-06-07T16:35:31Z
dc.title.none.fl_str_mv A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
title A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
spellingShingle A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
Camacho, Jorge
antibodies
DiaSorin immunoassay
neutralizing antibodies
receptor binding domain antibodies
Roche immunoassay
SARS-CoV-2
S-trimeric antibodies
title_short A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
title_full A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
title_fullStr A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
title_full_unstemmed A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
title_sort A performance comparison of two (electro) chemiluminescence immunoassays for detection and quantitation of serum anti-spike antibodies according to SARS-CoV-2 vaccination and infections status
dc.creator.none.fl_str_mv Camacho, Jorge
Albert, Eliseo
Zulaica, Joao
Alvarez-Rodriguez, Beatriz
Rusu, Luciana
Olea, Beatriz
Alcaraz, Maria Jesus
Geller, Ron
Gimenez, Estela
Navarro, David
author Camacho, Jorge
author_facet Camacho, Jorge
Albert, Eliseo
Zulaica, Joao
Alvarez-Rodriguez, Beatriz
Rusu, Luciana
Olea, Beatriz
Alcaraz, Maria Jesus
Geller, Ron
Gimenez, Estela
Navarro, David
author_role author
author2 Albert, Eliseo
Zulaica, Joao
Alvarez-Rodriguez, Beatriz
Rusu, Luciana
Olea, Beatriz
Alcaraz, Maria Jesus
Geller, Ron
Gimenez, Estela
Navarro, David
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv antibodies
DiaSorin immunoassay
neutralizing antibodies
receptor binding domain antibodies
Roche immunoassay
SARS-CoV-2
S-trimeric antibodies
topic antibodies
DiaSorin immunoassay
neutralizing antibodies
receptor binding domain antibodies
Roche immunoassay
SARS-CoV-2
S-trimeric antibodies
description The information provided by SARS-CoV-2 spike (S)-targeting immunoassays can be instrumental in clinical-decision making. We compared the performance of the Elecsys (R) Anti-SARS-CoV-2 S assay (Roche Diagnostics) and the LIAISON (R) SARS-CoV-2 TrimericS IgG assay (DiaSorin) using a total of 1176 sera from 797 individuals, of which 286 were from vaccinated-SARS-CoV-2/experienced (Vac-Ex), 581 from vaccinated/naive (Vac-N), 147 from unvaccinated/experienced (Unvac-Ex), and 162 from unvaccinated/naive (Unvac-N) individuals. The Roche assay returned a higher number of positive results (907 vs. 790; p = 0.45; overall sensitivity: 89.3% vs. 77.6%). The concordance between results provided by the two immunoassays was higher for sera from Vac-N (kappa: 0.58; interquartile ranges [IQR]: 0.50-0.65) than for sera from Vac-Ex (kappa: 0.19; IQR: -0.14 to 0.52) or Unvac-Ex (kappa: 0.18; IQR: 0.06-0.30). Discordant results occurred more frequently among sera from Unvac-Ex (34.7%) followed by Vac-N (14.6%) and Vac-Ex (2.7%). Antibody levels quantified by both immunoassays were not significantly different when <250 (p = 0.87) or <1000BAU/ml (p = 0.13); in contrast, for sera >= 1000BAU/ml, the Roche assay returned significantly higher values than the DiaSorin assay (p < 0.008). Neutralizing antibody titers (NtAb) were measured in 127 sera from Vac-Ex or Vac-N using a S-pseudotyped virus neutralization assay of Wuhan-Hu-1, Omicron BA.1, and Omicron BA.2. The correlation between antibody levels and NtAb titers was higher for sera from Vac-N than those from Vac-Ex, irrespective of the (sub)variant considered. In conclusion, neither qualitative nor quantitative results returned by both immunoassays are interchangeable. The performance of both assays was found to be greatly influenced by the vaccination and SARS-CoV-2 infection status of individuals.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/17040
url https://incliva.portalinvestigacion.com/publicaciones/17040
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv JOURNAL OF MEDICAL VIROLOGY
ISSN: 01466615
ISSNe: 10969071
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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