Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients
Background/objectives: In a previous study, we described elevated anti-Anisakis IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-Anisakis immunoglobulins in septic patients during hospital admission and their association...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/113488 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/113488 |
| Access Level: | acceso abierto |
| Palabra clave: | 576.8 ELISA anti-Anisakis antibodies apoptosis mortality sepsis αβ T-cells γδ T-cells Parasitología (Farmacia) 3207.12 Parasitología |
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Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic PatientsAndreu-Ballester, Juan CarlosNavarro, AmparoArribas, Miguel AngelRico, MoisesAlbert, LauraGarcía-Ballesteros, CarlosGalindo-Regal, LorenaSorando-Serra, RosaLópez-Chuliá, FranciscaPeydro, FedericoRodero Martínez, MartaGonzález Fernández, JuanCuéllar Del Hoyo, María Del Carmen576.8ELISAanti-Anisakis antibodiesapoptosismortalitysepsisαβ T-cellsγδ T-cellsParasitología (Farmacia)3207.12 ParasitologíaBackground/objectives: In a previous study, we described elevated anti-Anisakis IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-Anisakis immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets. Methods: We recruited 80 subjects: 40 patients with sepsis and 40 controls. αβ and γδ T cells were analyzed using flow cytometry. Apoptosis was also assessed, and anti-Anisakis antibodies were measured by ELISA in the sera of patients with sepsis and controls. Results: In the second analysis (7-10 after sepsis evolution), an increase in all specific antibody isotypes was identified in individuals with septic shock, except IgE. The levels of anti-Anisakis IgG and IgA were higher in the subjects with sepsis in the first analysis and continued to increase in the second analysis compared with the healthy control subjects. There was an increase in anti-Anisakis IgG and IgA levels in surviving patients and an increase in IgA levels in non-surviving patients. A rise in specific IgG and IgE levels was noted in the second analysis of patients with sepsis with αβ CD3+ T cell deficiency. Patients without γδ T cell deficiency had increased anti-Anisakis IgA levels 7-10 days after admission. Conclusions: Our results suggest a previous infection by Anisakis that could be involved in the subsequent septic process and be related to patients who have negative cultures in which the pathogen causing sepsis has not been identified.MDPIUniversidad Complutense de Madrid20242024-11-2720242024-11-27journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/113488reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1134882026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| title |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| spellingShingle |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients Andreu-Ballester, Juan Carlos 576.8 ELISA anti-Anisakis antibodies apoptosis mortality sepsis αβ T-cells γδ T-cells Parasitología (Farmacia) 3207.12 Parasitología |
| title_short |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| title_full |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| title_fullStr |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| title_full_unstemmed |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| title_sort |
Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients |
| dc.creator.none.fl_str_mv |
Andreu-Ballester, Juan Carlos Navarro, Amparo Arribas, Miguel Angel Rico, Moises Albert, Laura García-Ballesteros, Carlos Galindo-Regal, Lorena Sorando-Serra, Rosa López-Chuliá, Francisca Peydro, Federico Rodero Martínez, Marta González Fernández, Juan Cuéllar Del Hoyo, María Del Carmen |
| author |
Andreu-Ballester, Juan Carlos |
| author_facet |
Andreu-Ballester, Juan Carlos Navarro, Amparo Arribas, Miguel Angel Rico, Moises Albert, Laura García-Ballesteros, Carlos Galindo-Regal, Lorena Sorando-Serra, Rosa López-Chuliá, Francisca Peydro, Federico Rodero Martínez, Marta González Fernández, Juan Cuéllar Del Hoyo, María Del Carmen |
| author_role |
author |
| author2 |
Navarro, Amparo Arribas, Miguel Angel Rico, Moises Albert, Laura García-Ballesteros, Carlos Galindo-Regal, Lorena Sorando-Serra, Rosa López-Chuliá, Francisca Peydro, Federico Rodero Martínez, Marta González Fernández, Juan Cuéllar Del Hoyo, María Del Carmen |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
576.8 ELISA anti-Anisakis antibodies apoptosis mortality sepsis αβ T-cells γδ T-cells Parasitología (Farmacia) 3207.12 Parasitología |
| topic |
576.8 ELISA anti-Anisakis antibodies apoptosis mortality sepsis αβ T-cells γδ T-cells Parasitología (Farmacia) 3207.12 Parasitología |
| description |
Background/objectives: In a previous study, we described elevated anti-Anisakis IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-Anisakis immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets. Methods: We recruited 80 subjects: 40 patients with sepsis and 40 controls. αβ and γδ T cells were analyzed using flow cytometry. Apoptosis was also assessed, and anti-Anisakis antibodies were measured by ELISA in the sera of patients with sepsis and controls. Results: In the second analysis (7-10 after sepsis evolution), an increase in all specific antibody isotypes was identified in individuals with septic shock, except IgE. The levels of anti-Anisakis IgG and IgA were higher in the subjects with sepsis in the first analysis and continued to increase in the second analysis compared with the healthy control subjects. There was an increase in anti-Anisakis IgG and IgA levels in surviving patients and an increase in IgA levels in non-surviving patients. A rise in specific IgG and IgE levels was noted in the second analysis of patients with sepsis with αβ CD3+ T cell deficiency. Patients without γδ T cell deficiency had increased anti-Anisakis IgA levels 7-10 days after admission. Conclusions: Our results suggest a previous infection by Anisakis that could be involved in the subsequent septic process and be related to patients who have negative cultures in which the pathogen causing sepsis has not been identified. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-11-27 2024 2024-11-27 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/113488 |
| url |
https://hdl.handle.net/20.500.14352/113488 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
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reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
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Universidad Complutense de Madrid (UCM) |
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Docta Complutense |
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Docta Complutense |
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