Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal

We tested the targeting efficiency of magnetoliposomes (MLPs) labeled with tripeptide arginine-glycine-aspartic acid (RGD) on two types of cells: HeLa cells expressing RGD receptors and 3T3 cells lacking RGD receptors. The targeting ability of RGD-MLPs was compared to that of bare MLPs and MLPs stab...

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Detalles Bibliográficos
Autores: Estelrich i Latràs, Joan, Busquets i Viñas, Ma. Antonia, Morán Badenas, María del Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177599
Acceso en línea:https://hdl.handle.net/2445/177599
Access Level:acceso abierto
Palabra clave:Pèptids
Microscòpia
Polímers
Liposomes
Peptides
Microscopy
Polymers
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spelling Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed GoalEstelrich i Latràs, JoanBusquets i Viñas, Ma. AntoniaMorán Badenas, María del CarmenPèptidsMicroscòpiaPolímersLiposomesPeptidesMicroscopyPolymersLiposomesWe tested the targeting efficiency of magnetoliposomes (MLPs) labeled with tripeptide arginine-glycine-aspartic acid (RGD) on two types of cells: HeLa cells expressing RGD receptors and 3T3 cells lacking RGD receptors. The targeting ability of RGD-MLPs was compared to that of bare MLPs and MLPs stabilized with poly(ethylene glycol) (PEG). Cellular internalization of these liposomes was determined by flow cytometry and confocal microscopy, which showed that both types of cells took up more nontargeting MLPs than targeting RGD-MLPs or PEG-MLPs, with PEG-MLPs showing the lowest degree of internalization. The presence of specific receptors on HeLa cells did not facilitate the binding of RGD-MLPs, probably due to the presence of PEG chains on the liposomal surface. The polymer increases the circulation time of the liposomes in the organism but reduces their interactions with cells. Despite the localization of the RGD peptide on the tip of PEG in RGD-MLPs, the interaction between the liposome and cell was still limited. To avoid this drawback, targeting drug delivery systems can be prepared with two types of PEG: one of a short length to enable biocompatibility and the other of a longer chain to carry the ligand.American Chemical Society2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177599Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1021/acsomega.7b00778ACS Omega , 2017, vol. 2, num. 10, p. 6544-6555https://doi.org/10.1021/acsomega.7b00778(c) American Chemical Society, 2017info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1775992026-05-27T06:46:51Z
dc.title.none.fl_str_mv Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
title Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
spellingShingle Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
Estelrich i Latràs, Joan
Pèptids
Microscòpia
Polímers
Liposomes
Peptides
Microscopy
Polymers
Liposomes
title_short Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
title_full Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
title_fullStr Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
title_full_unstemmed Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
title_sort Effect of PEGylation on Ligand-Targeted Magnetoliposomes: A Missed Goal
dc.creator.none.fl_str_mv Estelrich i Latràs, Joan
Busquets i Viñas, Ma. Antonia
Morán Badenas, María del Carmen
author Estelrich i Latràs, Joan
author_facet Estelrich i Latràs, Joan
Busquets i Viñas, Ma. Antonia
Morán Badenas, María del Carmen
author_role author
author2 Busquets i Viñas, Ma. Antonia
Morán Badenas, María del Carmen
author2_role author
author
dc.subject.none.fl_str_mv Pèptids
Microscòpia
Polímers
Liposomes
Peptides
Microscopy
Polymers
Liposomes
topic Pèptids
Microscòpia
Polímers
Liposomes
Peptides
Microscopy
Polymers
Liposomes
description We tested the targeting efficiency of magnetoliposomes (MLPs) labeled with tripeptide arginine-glycine-aspartic acid (RGD) on two types of cells: HeLa cells expressing RGD receptors and 3T3 cells lacking RGD receptors. The targeting ability of RGD-MLPs was compared to that of bare MLPs and MLPs stabilized with poly(ethylene glycol) (PEG). Cellular internalization of these liposomes was determined by flow cytometry and confocal microscopy, which showed that both types of cells took up more nontargeting MLPs than targeting RGD-MLPs or PEG-MLPs, with PEG-MLPs showing the lowest degree of internalization. The presence of specific receptors on HeLa cells did not facilitate the binding of RGD-MLPs, probably due to the presence of PEG chains on the liposomal surface. The polymer increases the circulation time of the liposomes in the organism but reduces their interactions with cells. Despite the localization of the RGD peptide on the tip of PEG in RGD-MLPs, the interaction between the liposome and cell was still limited. To avoid this drawback, targeting drug delivery systems can be prepared with two types of PEG: one of a short length to enable biocompatibility and the other of a longer chain to carry the ligand.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177599
url https://hdl.handle.net/2445/177599
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acsomega.7b00778
ACS Omega , 2017, vol. 2, num. 10, p. 6544-6555
https://doi.org/10.1021/acsomega.7b00778
dc.rights.none.fl_str_mv (c) American Chemical Society, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Chemical Society, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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