Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000

Proteins belonging to the RsmA (regulator of secondary metabolism)/CsrA (carbon storage regulator) family are small RNA-binding proteins that play crucial roles post-transcriptionally regulating gene expression in many Gram-negative and some Gram-positive bacteria. Although most of the bacteria stud...

Full description

Bibliographic Details
Authors: Vásquez, Adriana, Ferreiro, María Dolores, Martínez-Rodríguez, Laura, Gallegos, María Trinidad
Format: article
Status:Published version
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/374332
Online Access:http://hdl.handle.net/10261/374332
Access Level:Open access
Keyword:Pseudomonas syringae
Rsm proteins
RsmA
CsrA
Gac-Rsm
RpoS
id ES_7cb133e2d73eac1764abc3f0f9be398f
oai_identifier_str oai:digital.csic.es:10261/374332
network_acronym_str ES
network_name_str España
repository_id_str
spelling Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000Vásquez, AdrianaFerreiro, María DoloresMartínez-Rodríguez, LauraGallegos, María TrinidadPseudomonas syringaeRsm proteinsRsmACsrAGac-RsmRpoSProteins belonging to the RsmA (regulator of secondary metabolism)/CsrA (carbon storage regulator) family are small RNA-binding proteins that play crucial roles post-transcriptionally regulating gene expression in many Gram-negative and some Gram-positive bacteria. Although most of the bacteria studied have a single RsmA/CsrA gene, Pseudomonas syringae pv. tomato (Pto) DC3000 encodes five Rsm proteins: RsmA/CsrA2, RsmC/CsrA1, RsmD/CsrA4, RsmE/CsrA3, and RsmH/CsrA5. This work aims to provide a comprehensive analysis of the expression of these five rsm protein-encoding genes, elucidate the regulatory mechanisms governing their expression, as well as the physiological relevance of each variant. To achieve this, we examined the expression of rsmA, rsmE, rsmC, rsmD, and rsmH within their genetic contexts, identified their promoter regions, and assessed the impact of both their deletion and overexpression on various Pto DC3000 phenotypes. A novel finding is that rsmA and rsmC are part of an operon with the upstream genes, whereas rsmH seems to be co-transcribed with two downstream genes. We also observed significant variability in expression levels and RpoS dependence among the five rsm paralogs. Thus, despite the extensive repertoire of rsm genes in Pto DC3000, only rsmA, rsmE and rsmH were significantly expressed under all tested conditions (swarming, minimal and T3SS-inducing liquid media). Among these, RsmE and RsmA were corroborated as the most important paralogs at the functional level, whereas RsmH played a minor role in regulating free life and plant-associated phenotypes. Conversely, RsmC and RsmD did not seem to be functional under the conditions tested.This research was funded by grant PID2021–122418NB-I00 (funded by MICIU/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”). M.D.F was supported by a FPU contract from the Spanish MECD/MEFP (ECD/1619/2013).Elsevier BVConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/374332reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122418NB-I00info:eu-repo/grantAgreement/MECD/MEFP/ECDThe underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1016/j.micres.2024.127926http://dx.doi.org/10.1016/j.micres.2024.127926Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3743322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
title Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
spellingShingle Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
Vásquez, Adriana
Pseudomonas syringae
Rsm proteins
RsmA
CsrA
Gac-Rsm
RpoS
title_short Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
title_full Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
title_fullStr Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
title_full_unstemmed Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
title_sort Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000
dc.creator.none.fl_str_mv Vásquez, Adriana
Ferreiro, María Dolores
Martínez-Rodríguez, Laura
Gallegos, María Trinidad
author Vásquez, Adriana
author_facet Vásquez, Adriana
Ferreiro, María Dolores
Martínez-Rodríguez, Laura
Gallegos, María Trinidad
author_role author
author2 Ferreiro, María Dolores
Martínez-Rodríguez, Laura
Gallegos, María Trinidad
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pseudomonas syringae
Rsm proteins
RsmA
CsrA
Gac-Rsm
RpoS
topic Pseudomonas syringae
Rsm proteins
RsmA
CsrA
Gac-Rsm
RpoS
description Proteins belonging to the RsmA (regulator of secondary metabolism)/CsrA (carbon storage regulator) family are small RNA-binding proteins that play crucial roles post-transcriptionally regulating gene expression in many Gram-negative and some Gram-positive bacteria. Although most of the bacteria studied have a single RsmA/CsrA gene, Pseudomonas syringae pv. tomato (Pto) DC3000 encodes five Rsm proteins: RsmA/CsrA2, RsmC/CsrA1, RsmD/CsrA4, RsmE/CsrA3, and RsmH/CsrA5. This work aims to provide a comprehensive analysis of the expression of these five rsm protein-encoding genes, elucidate the regulatory mechanisms governing their expression, as well as the physiological relevance of each variant. To achieve this, we examined the expression of rsmA, rsmE, rsmC, rsmD, and rsmH within their genetic contexts, identified their promoter regions, and assessed the impact of both their deletion and overexpression on various Pto DC3000 phenotypes. A novel finding is that rsmA and rsmC are part of an operon with the upstream genes, whereas rsmH seems to be co-transcribed with two downstream genes. We also observed significant variability in expression levels and RpoS dependence among the five rsm paralogs. Thus, despite the extensive repertoire of rsm genes in Pto DC3000, only rsmA, rsmE and rsmH were significantly expressed under all tested conditions (swarming, minimal and T3SS-inducing liquid media). Among these, RsmE and RsmA were corroborated as the most important paralogs at the functional level, whereas RsmH played a minor role in regulating free life and plant-associated phenotypes. Conversely, RsmC and RsmD did not seem to be functional under the conditions tested.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/374332
url http://hdl.handle.net/10261/374332
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122418NB-I00
info:eu-repo/grantAgreement/MECD/MEFP/ECD
The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1016/j.micres.2024.127926
http://dx.doi.org/10.1016/j.micres.2024.127926
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411609453002752
score 15.811543

Similar Items