Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.

Saati Santamaría, Zaki; López-Mondéjar, Rubén; Jiménez Gómez, Alejandro; Díez Méndez, Alexandra; Větrovský, Tomáš; Igual, José M.; Velázquez Pérez, María Encarnación; Kolařik, Miroslav; Rivas González, Raúl; García Fraile, Paula

Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.

[EN]Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments formicrobial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs....

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Autores:	Saati Santamaría, Zaki, López-Mondéjar, Rubén, Jiménez Gómez, Alejandro, Díez Méndez, Alexandra, Větrovský, Tomáš, Igual, José M., Velázquez Pérez, María Encarnación, Kolařik, Miroslav, Rivas González, Raúl, García Fraile, Paula
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2018
País:	España
Institución:	Universidad de Salamanca (USAL)
Repositorio:	GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:	oai:gredos.usal.es:10366/154226
Acceso en línea:	http://hdl.handle.net/10366/154226
Access Level:	acceso abierto
Palabra clave:	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Microbiology Environmental Microbiology 2414 Microbiología Microbiología microbiología ambiental

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spelling	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.Saati Santamaría, ZakiLópez-Mondéjar, RubénJiménez Gómez, AlejandroDíez Méndez, AlexandraVětrovský, TomášIgual, José M.Velázquez Pérez, María EncarnaciónKolařik, MiroslavRivas González, RaúlGarcía Fraile, PaulaAntimicrobialsAnticarcinogenicAntiviralGenome miningBark beetlesAntibiotic resistanceNRPS-PKSSecondary metabolitesMicrobiologyEnvironmental Microbiology2414 MicrobiologíaMicrobiologíamicrobiología ambiental[EN]Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments formicrobial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists. In this study the capacity of several bacterial strains, isolated from the bark beetles Ips acuminatus, Pityophthorus pityographus Cryphalus piceae, and Pityogenes bidentatus, to produce antimicrobial compounds was analyzed. Several isolates exhibited the capacity to inhibit Gram-positive and Gram-negative bacteria, as well as fungi. The genome sequence analysis of three Pseudomonas isolates predicted the presence of several gene clusters implicated in the production of already described antimicrobials and moreover, the low similarity of some of these clusters with those previously described, suggests that they encode new undescribed substances, which may be useful for developing new antimicrobial agents. Moreover, these bacteria appear to have genetic machinery for producing antitumoral and antiviral substances. Finally, the strain IA19T showed to represent a new species of the genus Pseudomonas. The 16S rRNA gene sequence analysis showed that its most closely related species include Pseudomonas lutea, Pseudomonas graminis, Pseudomonas abietaniphila and Pseudomonas alkylphenolica, with 98.6, 98.5 98.4, and 98.4%identity, respectively. MLSA of the housekeeping genes gyrB, rpoB, and rpoD confirmed that strain IA19T clearly separates from its closest related species. Average nucleotide identity between strains IA19T and P. abietaniphila ATCC 700689T, P. graminis DSM 11363T, P. alkylphenolica KL28T and P. lutea DSM 17257T were 85.3, 80.2, 79.0, and 72.1%, respectively. Growth occurs at 4-37◦C and pH 6.5-8. Optimal growth occurs at 28◦C, pH 7–8 and up to 2.5%NaCl. Respiratory ubiquinones are Q9 (97%) and Q8 (3%). C16:0 and in summed feature 3 are the main fatty acids. Based on genotypic, phenotypic and chemotaxonomic characteristics, the description of Pseudomonas bohemica sp. nov. has been proposed. The type strain is IA19T (=CECT 9403T=LMG 30182T).Frontiers202420242018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/154226reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)Inglés16-15293YAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1542262026-06-07T06:28:51Z
dc.title.none.fl_str_mv	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
title	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
spellingShingle	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov. Saati Santamaría, Zaki Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Microbiology Environmental Microbiology 2414 Microbiología Microbiología microbiología ambiental
title_short	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
title_full	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
title_fullStr	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
title_full_unstemmed	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
title_sort	Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.
dc.creator.none.fl_str_mv	Saati Santamaría, Zaki López-Mondéjar, Rubén Jiménez Gómez, Alejandro Díez Méndez, Alexandra Větrovský, Tomáš Igual, José M. Velázquez Pérez, María Encarnación Kolařik, Miroslav Rivas González, Raúl García Fraile, Paula
author	Saati Santamaría, Zaki
author_facet	Saati Santamaría, Zaki López-Mondéjar, Rubén Jiménez Gómez, Alejandro Díez Méndez, Alexandra Větrovský, Tomáš Igual, José M. Velázquez Pérez, María Encarnación Kolařik, Miroslav Rivas González, Raúl García Fraile, Paula
author_role	author
author2	López-Mondéjar, Rubén Jiménez Gómez, Alejandro Díez Méndez, Alexandra Větrovský, Tomáš Igual, José M. Velázquez Pérez, María Encarnación Kolařik, Miroslav Rivas González, Raúl García Fraile, Paula
author2_role	author author author author author author author author author
dc.subject.none.fl_str_mv	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Microbiology Environmental Microbiology 2414 Microbiología Microbiología microbiología ambiental
topic	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Microbiology Environmental Microbiology 2414 Microbiología Microbiología microbiología ambiental
description	[EN]Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments formicrobial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists. In this study the capacity of several bacterial strains, isolated from the bark beetles Ips acuminatus, Pityophthorus pityographus Cryphalus piceae, and Pityogenes bidentatus, to produce antimicrobial compounds was analyzed. Several isolates exhibited the capacity to inhibit Gram-positive and Gram-negative bacteria, as well as fungi. The genome sequence analysis of three Pseudomonas isolates predicted the presence of several gene clusters implicated in the production of already described antimicrobials and moreover, the low similarity of some of these clusters with those previously described, suggests that they encode new undescribed substances, which may be useful for developing new antimicrobial agents. Moreover, these bacteria appear to have genetic machinery for producing antitumoral and antiviral substances. Finally, the strain IA19T showed to represent a new species of the genus Pseudomonas. The 16S rRNA gene sequence analysis showed that its most closely related species include Pseudomonas lutea, Pseudomonas graminis, Pseudomonas abietaniphila and Pseudomonas alkylphenolica, with 98.6, 98.5 98.4, and 98.4%identity, respectively. MLSA of the housekeeping genes gyrB, rpoB, and rpoD confirmed that strain IA19T clearly separates from its closest related species. Average nucleotide identity between strains IA19T and P. abietaniphila ATCC 700689T, P. graminis DSM 11363T, P. alkylphenolica KL28T and P. lutea DSM 17257T were 85.3, 80.2, 79.0, and 72.1%, respectively. Growth occurs at 4-37◦C and pH 6.5-8. Optimal growth occurs at 28◦C, pH 7–8 and up to 2.5%NaCl. Respiratory ubiquinones are Q9 (97%) and Q8 (3%). C16:0 and in summed feature 3 are the main fatty acids. Based on genotypic, phenotypic and chemotaxonomic characteristics, the description of Pseudomonas bohemica sp. nov. has been proposed. The type strain is IA19T (=CECT 9403T=LMG 30182T).
publishDate	2018
dc.date.none.fl_str_mv	2018 2024 2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10366/154226
url	http://hdl.handle.net/10366/154226
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	16-15293Y
dc.rights.none.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Frontiers
publisher.none.fl_str_mv	Frontiers
dc.source.none.fl_str_mv	reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL)
instname_str	Universidad de Salamanca (USAL)
reponame_str	GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection	GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Pseudomonas bohemica sp. nov.

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