Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels

Understanding the effects of soil amendments and low disturbance practices on soil health, nutrient cycling and microbial activity is essential for improving agricultural sustainability. Ramial chipped wood (RCW) is a promising carbon-rich organic soil amendment but its effects on microbial activity...

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Autores: González-Coria, Johana, Ioan, Michelle-Danielle, Hohmann, Pierre, Segarra Braunstein, Guillem, Pérez Llorca, Marina, Pérez Bosch, Maria, Vallverdú i Queralt, Anna, Romanyà i Socoró, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/222236
Acceso en línea:https://hdl.handle.net/2445/222236
Access Level:acceso abierto
Palabra clave:Biogeoquímica
Sòls agrícoles
Microbiologia
Biogeochemistry
Rural land use
Microbiology
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spelling Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levelsGonzález-Coria, JohanaIoan, Michelle-DanielleHohmann, PierreSegarra Braunstein, GuillemPérez Llorca, MarinaPérez Bosch, MariaVallverdú i Queralt, AnnaRomanyà i Socoró, JoanBiogeoquímicaSòls agrícolesMicrobiologiaBiogeochemistryRural land useMicrobiologyUnderstanding the effects of soil amendments and low disturbance practices on soil health, nutrient cycling and microbial activity is essential for improving agricultural sustainability. Ramial chipped wood (RCW) is a promising carbon-rich organic soil amendment but its effects on microbial activity, nitrogen (N) cycling genes and microbial taxa, particularly across soil depth, remain poorly understood. This study aimed to evaluate the short-term effects of RCW applications following a no-till practice on various soil properties including microbial composition and N cycling genes, during the second year after RCW incorporation. The experiment was conducted using tomato (<em>Solanum lycopersicum</em>) as a crop species in the Mediterranean region.   We compared the surface (0-20 cm) and subsurface (20-25 cm) metagenomes of RCW-treated soils with those treated with standard N-rich organic pellet, as a control, (CTL) and compost (CMP).</p><p>RCW, particularly at high doses (RCW-HD), increased soil organic carbon and microbial biomass at an early stage. Despite a 50% reduction in organic fertiliser use, RCW-HD did not reduce N availability and crop productivity, suggesting improved N use efficiency. Several N-cycling gene abundances were elevated under CTL compared to RCW-HD, including the nitrification-related <em>pmoA-amoA</em> (+42%) and <em>pmoC-amoC</em> (+72%), and the denitrification-related<em> nosZ </em>(+14%). The RCW-HD no-till system increased nitrate reduction assimilation (+13% <em>nrtABC</em>) and favoured N-fixing bacterial genera such as <em>Terrihabitans,</em> <em>Ferriphaselus, Azospira </em>and <em>Rhodopseudomonas</em>. Soil depth significantly influenced 72% of the N-cycling genes, with key genes being more abundant at the surface. These results highlight the potential of RCW to improve N retention and soil fertility, while reducing fertiliser dependence and greenhouse gas emissions. They also support sustainable practices in regenerative agriculture by highlighting how microbiomes contribute to the efficiency of nitrogen cycling. </p><p><em>Keywords:</em> Shotgun metagenomics; Biogeochemical process; Microbial activity; Organic agriculture; Soil organic matter; Soil regeneration.Elsevier B.V.2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/222236Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.still.2025.106729Soil & Tillage Research, 2025, vol. 254https://doi.org/10.1016/j.still.2025.106729cc-by-nc-nd (c) Johana González-Coria, et al., 2025http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2222362026-05-27T06:46:51Z
dc.title.none.fl_str_mv Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
title Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
spellingShingle Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
González-Coria, Johana
Biogeoquímica
Sòls agrícoles
Microbiologia
Biogeochemistry
Rural land use
Microbiology
title_short Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
title_full Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
title_fullStr Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
title_full_unstemmed Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
title_sort Early-stage effects of carbon-rich soil amendments stimulate retention-related nitrogen genes while maintaining nitrogen and yield levels
dc.creator.none.fl_str_mv González-Coria, Johana
Ioan, Michelle-Danielle
Hohmann, Pierre
Segarra Braunstein, Guillem
Pérez Llorca, Marina
Pérez Bosch, Maria
Vallverdú i Queralt, Anna
Romanyà i Socoró, Joan
author González-Coria, Johana
author_facet González-Coria, Johana
Ioan, Michelle-Danielle
Hohmann, Pierre
Segarra Braunstein, Guillem
Pérez Llorca, Marina
Pérez Bosch, Maria
Vallverdú i Queralt, Anna
Romanyà i Socoró, Joan
author_role author
author2 Ioan, Michelle-Danielle
Hohmann, Pierre
Segarra Braunstein, Guillem
Pérez Llorca, Marina
Pérez Bosch, Maria
Vallverdú i Queralt, Anna
Romanyà i Socoró, Joan
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biogeoquímica
Sòls agrícoles
Microbiologia
Biogeochemistry
Rural land use
Microbiology
topic Biogeoquímica
Sòls agrícoles
Microbiologia
Biogeochemistry
Rural land use
Microbiology
description Understanding the effects of soil amendments and low disturbance practices on soil health, nutrient cycling and microbial activity is essential for improving agricultural sustainability. Ramial chipped wood (RCW) is a promising carbon-rich organic soil amendment but its effects on microbial activity, nitrogen (N) cycling genes and microbial taxa, particularly across soil depth, remain poorly understood. This study aimed to evaluate the short-term effects of RCW applications following a no-till practice on various soil properties including microbial composition and N cycling genes, during the second year after RCW incorporation. The experiment was conducted using tomato (<em>Solanum lycopersicum</em>) as a crop species in the Mediterranean region.   We compared the surface (0-20 cm) and subsurface (20-25 cm) metagenomes of RCW-treated soils with those treated with standard N-rich organic pellet, as a control, (CTL) and compost (CMP).</p><p>RCW, particularly at high doses (RCW-HD), increased soil organic carbon and microbial biomass at an early stage. Despite a 50% reduction in organic fertiliser use, RCW-HD did not reduce N availability and crop productivity, suggesting improved N use efficiency. Several N-cycling gene abundances were elevated under CTL compared to RCW-HD, including the nitrification-related <em>pmoA-amoA</em> (+42%) and <em>pmoC-amoC</em> (+72%), and the denitrification-related<em> nosZ </em>(+14%). The RCW-HD no-till system increased nitrate reduction assimilation (+13% <em>nrtABC</em>) and favoured N-fixing bacterial genera such as <em>Terrihabitans,</em> <em>Ferriphaselus, Azospira </em>and <em>Rhodopseudomonas</em>. Soil depth significantly influenced 72% of the N-cycling genes, with key genes being more abundant at the surface. These results highlight the potential of RCW to improve N retention and soil fertility, while reducing fertiliser dependence and greenhouse gas emissions. They also support sustainable practices in regenerative agriculture by highlighting how microbiomes contribute to the efficiency of nitrogen cycling. </p><p><em>Keywords:</em> Shotgun metagenomics; Biogeochemical process; Microbial activity; Organic agriculture; Soil organic matter; Soil regeneration.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/222236
url https://hdl.handle.net/2445/222236
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.still.2025.106729
Soil & Tillage Research, 2025, vol. 254
https://doi.org/10.1016/j.still.2025.106729
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Johana González-Coria, et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Johana González-Coria, et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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