Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs

New adjuvants based on microparticles are being developed for use in fish vaccines. The size of the microparticles may affect the immune response generated, as the adjuvant can either be retained at the site of injection or transported to lymphoid organs. The objectives of this study were to evaluat...

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Detalles Bibliográficos
Autores: Folgueira López, Iria, Noia Guldrís, Manuel, Blanco Abad, Verónica, Mallo Seijas, Natalia, Leiro Vidal, José Manuel, Lamas Fernández, Jesús
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/45959
Acceso en línea:https://hdl.handle.net/10347/45959
Access Level:acceso abierto
Palabra clave:Fish
Peritoneum
Adjuvant
Microparticles
Phagocytes
Migration
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spelling Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organsFolgueira López, IriaNoia Guldrís, ManuelBlanco Abad, VerónicaMallo Seijas, NataliaLeiro Vidal, José ManuelLamas Fernández, JesúsFishPeritoneumAdjuvantMicroparticlesPhagocytesMigrationNew adjuvants based on microparticles are being developed for use in fish vaccines. The size of the microparticles may affect the immune response generated, as the adjuvant can either be retained at the site of injection or transported to lymphoid organs. The objectives of this study were to evaluate the maximum size of particles that can be exported out of the cavity, to determine the phagocytosis kinetics and to establish the routes whereby particle-containing cells move from the peritoneal cavity after injection. Fish were injected intraperitoneally with fluorescent cyclodextrins or with fluorescent particles of different size (0.1–10 μm). Phagocytes containing beads of size 4 μm or larger did not reach lymphoid organs, although some were able to cross the peritoneal mesothelium. The number of free peritoneal neutrophils and macrophage-like cells containing beads peaked at 6 and 24 h respectively, and the numbers then decreased quickly, indicating migration of cells to the peritoneum or other body areas. Migration of cells containing beads mainly occurs through the visceral peritoneum. These cells were found on the latero-ventral surfaces of the peritoneal folds that connect the visceral organs. Except for some vascularised areas, the surfaces of liver, stomach and intestine were devoid of particle-containing cells. Some cells containing beads were also found attached to the parietal peritoneum, although in lower numbers than in the visceral peritoneum. Such cells were also found in high numbers in the spleen and kidney 6 h post injection. Because cells containing phagocytosed material quickly become attached to the peritoneum or migrate to lymphoid organs, the immune response generated by a vaccine or by an inflammatory stimulus should probably be evaluated in attached cells as well as in free peritoneal cells.ElsevierUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía20152015-04-0120152015-04-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/45959reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengMinisterio de Educación y Ciencia Not available INCITE07%2FPX1203039ESMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Programa Nacional de Investigación Fundamental AGL2011-29927-C02-01 ACTIVIDAD MODULADORA DEL RESVERATROL SOBRE LA INFLAMACION INDUCIDA POR ESCUTICOCILIADOS EN EL RODABALLOMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Programa Nacional de Investigación Fundamental AGL2010-21219%2FACUopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/459592026-06-15T12:47:27Z
dc.title.none.fl_str_mv Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
title Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
spellingShingle Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
Folgueira López, Iria
Fish
Peritoneum
Adjuvant
Microparticles
Phagocytes
Migration
title_short Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
title_full Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
title_fullStr Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
title_full_unstemmed Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
title_sort Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
dc.creator.none.fl_str_mv Folgueira López, Iria
Noia Guldrís, Manuel
Blanco Abad, Verónica
Mallo Seijas, Natalia
Leiro Vidal, José Manuel
Lamas Fernández, Jesús
author Folgueira López, Iria
author_facet Folgueira López, Iria
Noia Guldrís, Manuel
Blanco Abad, Verónica
Mallo Seijas, Natalia
Leiro Vidal, José Manuel
Lamas Fernández, Jesús
author_role author
author2 Noia Guldrís, Manuel
Blanco Abad, Verónica
Mallo Seijas, Natalia
Leiro Vidal, José Manuel
Lamas Fernández, Jesús
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.subject.none.fl_str_mv Fish
Peritoneum
Adjuvant
Microparticles
Phagocytes
Migration
topic Fish
Peritoneum
Adjuvant
Microparticles
Phagocytes
Migration
description New adjuvants based on microparticles are being developed for use in fish vaccines. The size of the microparticles may affect the immune response generated, as the adjuvant can either be retained at the site of injection or transported to lymphoid organs. The objectives of this study were to evaluate the maximum size of particles that can be exported out of the cavity, to determine the phagocytosis kinetics and to establish the routes whereby particle-containing cells move from the peritoneal cavity after injection. Fish were injected intraperitoneally with fluorescent cyclodextrins or with fluorescent particles of different size (0.1–10 μm). Phagocytes containing beads of size 4 μm or larger did not reach lymphoid organs, although some were able to cross the peritoneal mesothelium. The number of free peritoneal neutrophils and macrophage-like cells containing beads peaked at 6 and 24 h respectively, and the numbers then decreased quickly, indicating migration of cells to the peritoneum or other body areas. Migration of cells containing beads mainly occurs through the visceral peritoneum. These cells were found on the latero-ventral surfaces of the peritoneal folds that connect the visceral organs. Except for some vascularised areas, the surfaces of liver, stomach and intestine were devoid of particle-containing cells. Some cells containing beads were also found attached to the parietal peritoneum, although in lower numbers than in the visceral peritoneum. Such cells were also found in high numbers in the spleen and kidney 6 h post injection. Because cells containing phagocytosed material quickly become attached to the peritoneum or migrate to lymphoid organs, the immune response generated by a vaccine or by an inflammatory stimulus should probably be evaluated in attached cells as well as in free peritoneal cells.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-04-01
2015
2015-04-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/45959
url https://hdl.handle.net/10347/45959
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia Not available INCITE07%2FPX1203039ES
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Programa Nacional de Investigación Fundamental AGL2011-29927-C02-01 ACTIVIDAD MODULADORA DEL RESVERATROL SOBRE LA INFLAMACION INDUCIDA POR ESCUTICOCILIADOS EN EL RODABALLO
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Programa Nacional de Investigación Fundamental AGL2010-21219%2FACU
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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