Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains

15 pages.-- et al.

Detalles Bibliográficos
Autores: Carilla-Latorre, Sergio, Calvo-Garrido, Javier, Martínez, José L., Escalante, Ricardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/6319
Acceso en línea:http://hdl.handle.net/10261/6319
Access Level:acceso abierto
Palabra clave:Bacterial pathogens
Infective process
Pseudomonas aeruginosa
Strains
Dictyostelium discoideum
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spelling Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strainsCarilla-Latorre, SergioCalvo-Garrido, JavierMartínez, José L.Escalante, RicardoBacterial pathogensInfective processPseudomonas aeruginosaStrainsDictyostelium discoideum15 pages.-- et al.[Background]: Pseudomonas aeruginosa is one of the most relevant human opportunistic bacterial pathogens. Two strains (PAO1 and PA14) have been mainly used as models for studying virulence of P. aeruginosa. The strain PA14 is more virulent than PAO1 in a wide range of hosts including insects, nematodes and plants. Whereas some of the differences might be attributable to concerted action of determinants encoded in pathogenicity islands present in the genome of PA14, a global analysis of the differential host responses to these P. aeruginosa strains has not been addressed. Little is known about the host response to infection with P. aeruginosa and whether or not the global host transcription is being affected as a defense mechanism or altered in the benefit of the pathogen. Since the social amoeba Dictyostelium discoideum is a suitable host to study virulence of P. aeruginosa and other pathogens, we used available genomic tools in this model system to study the transcriptional host response to P. aeruginosa infection.[Results]: We have compared the virulence of the P. aeruginosa PAO1 and PA14 using D. discoideum and studied the transcriptional response of the amoeba upon infection. Our results showed that PA14 is more virulent in Dictyostelium than PA01using different plating assays. For studying the differential response of the host to infection by these model strains, D. discoideum cells were exposed to either P. aeruginosa PAO1 or P. aeruginosa PA14 (mixed with an excess of the non-pathogenic bacterium Klebsiella aerogenes as food supply) and after 4 hours, cellular RNA extracted. A three-way comparison was made using whole-genome D. discoideum microarrays between RNA samples from cells treated with the two different strains and control cells exposed only to K. aerogenes. The transcriptomic analyses have shown the existence of common and specific responses to infection. The expression of 364 genes changed in a similar way upon infection with one or another strain, whereas 169 genes were differentially regulated depending on whether the infecting strain was either P. aeruginosa PAO1 or PA14. Effects on metabolism, signalling, stress response and cell cycle can be inferred from the genes affected.[Conclusion]: Our results show that pathogenic Pseudomonas strains invoke both a common transcriptional response from Dictyostelium and a strain specific one, indicating that the infective process of bacterial pathogens can be strain-specific and is more complex than previously thought.This work was supported by grants BMC2006-00394 from the Spanish Ministerio de Educación y Ciencia (R.E.), CCG07-CSIC/SAL-1959 from Comunidad de Madrid/CSIC (R.E.) and BIO2005-04278, LSHM-CT-2005-518152 and LSHM-CT-2005-018705 to J.M.Peer reviewedBioMed Central200820082008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersion2495479 bytes3439104 bytes178176 bytes91648 bytesapplication/pdfapplication/vnd.ms-excelapplication/vnd.ms-excelapplication/vnd.ms-excelhttp://hdl.handle.net/10261/6319reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/1471-2180-8-109info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/63192026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
title Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
spellingShingle Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
Carilla-Latorre, Sergio
Bacterial pathogens
Infective process
Pseudomonas aeruginosa
Strains
Dictyostelium discoideum
title_short Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
title_full Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
title_fullStr Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
title_full_unstemmed Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
title_sort Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
dc.creator.none.fl_str_mv Carilla-Latorre, Sergio
Calvo-Garrido, Javier
Martínez, José L.
Escalante, Ricardo
author Carilla-Latorre, Sergio
author_facet Carilla-Latorre, Sergio
Calvo-Garrido, Javier
Martínez, José L.
Escalante, Ricardo
author_role author
author2 Calvo-Garrido, Javier
Martínez, José L.
Escalante, Ricardo
author2_role author
author
author
dc.subject.none.fl_str_mv Bacterial pathogens
Infective process
Pseudomonas aeruginosa
Strains
Dictyostelium discoideum
topic Bacterial pathogens
Infective process
Pseudomonas aeruginosa
Strains
Dictyostelium discoideum
description 15 pages.-- et al.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/6319
url http://hdl.handle.net/10261/6319
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/1471-2180-8-109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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3439104 bytes
178176 bytes
91648 bytes
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application/vnd.ms-excel
application/vnd.ms-excel
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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