Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor

An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized...

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Detalles Bibliográficos
Autores: Pinacho, Daniel G., Sánchez Baeza, Francisco J., Pividori, María Isabel, Marco, María Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/130884
Acceso en línea:http://hdl.handle.net/10261/130884
Access Level:acceso abierto
Palabra clave:fluoroquinolone antibiotics
Food safety
milk
antimicrobial residues
electrochemical immunosensor
magnetic beads
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spelling Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensorPinacho, Daniel G.Sánchez Baeza, Francisco J.Pividori, María IsabelMarco, María Pilarfluoroquinolone antibioticsFood safetymilkantimicrobial residueselectrochemical immunosensormagnetic beadsAn amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples. © 2014 by the authors; licensee MDPI, Basel, Switzerland.This work has been supported by the Ministry of Science and Innovation (Spain) (Contract number AGL2008-05578-C05-01 and by the European Community (FP7-FOOD-2007-211326 and FP7-OCEAN-2013-1-614168). The Nb4D group is a consolidated Grup de Recerca de la Generalitat de Catalunya and has support from the Departament d’Universitats, Recerca i Societat de la Informació la Generalitat de Catalunya (expedient 2014 SGR 1484). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, IniciativaIngenio 2010, Consolider Program, CIBER Actions and is financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Foundation. DG had a fellowship from the Fundación EROSKI.Peer ReviewedMolecular Diversity Preservation InternationalMinisterio de Ciencia e Innovación (España)European CommissionGeneralitat de CatalunyaInstituto de Salud Carlos IIIFundación EROSKIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620142016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/130884reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/s140915965Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1308842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
title Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
spellingShingle Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
Pinacho, Daniel G.
fluoroquinolone antibiotics
Food safety
milk
antimicrobial residues
electrochemical immunosensor
magnetic beads
title_short Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
title_full Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
title_fullStr Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
title_full_unstemmed Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
title_sort Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensor
dc.creator.none.fl_str_mv Pinacho, Daniel G.
Sánchez Baeza, Francisco J.
Pividori, María Isabel
Marco, María Pilar
author Pinacho, Daniel G.
author_facet Pinacho, Daniel G.
Sánchez Baeza, Francisco J.
Pividori, María Isabel
Marco, María Pilar
author_role author
author2 Sánchez Baeza, Francisco J.
Pividori, María Isabel
Marco, María Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Generalitat de Catalunya
Instituto de Salud Carlos III
Fundación EROSKI
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv fluoroquinolone antibiotics
Food safety
milk
antimicrobial residues
electrochemical immunosensor
magnetic beads
topic fluoroquinolone antibiotics
Food safety
milk
antimicrobial residues
electrochemical immunosensor
magnetic beads
description An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/130884
url http://hdl.handle.net/10261/130884
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/s140915965
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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