Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents

The use of nanomaterials in the field of bone tissue engineering implants is continuously dealing with the development of innovative solutions to common problems, as infection by colonization with common microbial agents, antibiotic bacterial resistance, and the formation of new bone tissue. Among t...

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Detalles Bibliográficos
Autores: Garino, Nadia, Sanvitale, Pasquale, Dumontel, Bianca, Laurenti, Marco, Colilla Nieto, Montserrat, Izquierdo Barba, Isabel, Cauda, Valentina, Vallet Regí, María Dulce Nombre
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/13250
Acceso en línea:https://hdl.handle.net/20.500.14352/13250
Access Level:acceso abierto
Palabra clave:615.46
546
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
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spelling Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agentsGarino, NadiaSanvitale, PasqualeDumontel, BiancaLaurenti, MarcoColilla Nieto, MontserratIzquierdo Barba, IsabelCauda, ValentinaVallet Regí, María Dulce Nombre615.46546MaterialesQuímica inorgánica (Farmacia)3312 Tecnología de MaterialesThe use of nanomaterials in the field of bone tissue engineering implants is continuously dealing with the development of innovative solutions to common problems, as infection by colonization with common microbial agents, antibiotic bacterial resistance, and the formation of new bone tissue. Among them, ZnO nanostructures are promising candidates thanks to their intrinsic antimicrobial activity and high biocompatibility. In this paper we aim to analyse the behaviour of ZnO nanocrystals (ZnO NCs), prepared with a new synthetic approach and not embedded in any composite matrix, for bone implants applications in-vitro. In particular, we have developed a novel, fast and reproducible microwave-assisted synthesis, obtaining highly-crystalline, round-shaped ZnO NCs of 20 nm in diameter as an extremely-stable colloidal solution in ethanol. Part of them were also chemically functionalized by anchoring amino-propyl groups to the ZnO surface (ZnO-NH2 NCs). Thus, the role of both ZnO NCs concentration and surface chemistry are tested in terms of biocompatibility towards pre-osteoblasts cells, promotion of cell proliferation and differentiation, and also in terms of antimicrobial activity against Gram positive and negative bacteria, such as Escherichia coli and Staphylococcus aureus, respectively. The results propose the ZnO-NH2 NCs as the most promising candidate to solve infections disease in bone implants and promote bone tissue proliferation at the same time, even at high concentrations. Whereas further investigations are needed for example to clarify the mechanism inhibiting biofilm formation and to investigate their role in in-vivo assays, we demonstrated that a fine and reproducible control over the chemical and structural parameters in ZnO nanomaterials can open new horizons in the use of functionalized ZnO NCs as a highly biocompatible and osteoinductive nanoantibiotic agent for bone tissue engineering.Royal Society of ChemistryUniversidad Complutense de Madrid20192019-01-0120192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/13250reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/132502026-06-02T12:44:21Z
dc.title.none.fl_str_mv Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
title Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
spellingShingle Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
Garino, Nadia
615.46
546
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
title_short Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
title_full Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
title_fullStr Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
title_full_unstemmed Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
title_sort Zinc oxide nanocrystals as nano-antibiotic and osteoinductive agents
dc.creator.none.fl_str_mv Garino, Nadia
Sanvitale, Pasquale
Dumontel, Bianca
Laurenti, Marco
Colilla Nieto, Montserrat
Izquierdo Barba, Isabel
Cauda, Valentina
Vallet Regí, María Dulce Nombre
author Garino, Nadia
author_facet Garino, Nadia
Sanvitale, Pasquale
Dumontel, Bianca
Laurenti, Marco
Colilla Nieto, Montserrat
Izquierdo Barba, Isabel
Cauda, Valentina
Vallet Regí, María Dulce Nombre
author_role author
author2 Sanvitale, Pasquale
Dumontel, Bianca
Laurenti, Marco
Colilla Nieto, Montserrat
Izquierdo Barba, Isabel
Cauda, Valentina
Vallet Regí, María Dulce Nombre
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.46
546
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
topic 615.46
546
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
description The use of nanomaterials in the field of bone tissue engineering implants is continuously dealing with the development of innovative solutions to common problems, as infection by colonization with common microbial agents, antibiotic bacterial resistance, and the formation of new bone tissue. Among them, ZnO nanostructures are promising candidates thanks to their intrinsic antimicrobial activity and high biocompatibility. In this paper we aim to analyse the behaviour of ZnO nanocrystals (ZnO NCs), prepared with a new synthetic approach and not embedded in any composite matrix, for bone implants applications in-vitro. In particular, we have developed a novel, fast and reproducible microwave-assisted synthesis, obtaining highly-crystalline, round-shaped ZnO NCs of 20 nm in diameter as an extremely-stable colloidal solution in ethanol. Part of them were also chemically functionalized by anchoring amino-propyl groups to the ZnO surface (ZnO-NH2 NCs). Thus, the role of both ZnO NCs concentration and surface chemistry are tested in terms of biocompatibility towards pre-osteoblasts cells, promotion of cell proliferation and differentiation, and also in terms of antimicrobial activity against Gram positive and negative bacteria, such as Escherichia coli and Staphylococcus aureus, respectively. The results propose the ZnO-NH2 NCs as the most promising candidate to solve infections disease in bone implants and promote bone tissue proliferation at the same time, even at high concentrations. Whereas further investigations are needed for example to clarify the mechanism inhibiting biofilm formation and to investigate their role in in-vivo assays, we demonstrated that a fine and reproducible control over the chemical and structural parameters in ZnO nanomaterials can open new horizons in the use of functionalized ZnO NCs as a highly biocompatible and osteoinductive nanoantibiotic agent for bone tissue engineering.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/13250
url https://hdl.handle.net/20.500.14352/13250
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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