Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content

Higher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation of branched-chain amino acids (BCAAs) in vegan diets can reduce risks associated to their deficiency, particularly in older adults, which can cause loss of skeletal mus...

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Detalles Bibliográficos
Autores: Vázquez-Vilar, Marta, Fernandez-Del-Carmen, Asun, Garcia-Carpintero, Victor, Drapal, Margit, Presa, Silvia, Ricci, Dorotea, Diretto, Gianfranco, Rambla, José Luis, Fernández-Muñoz, Rafael, Espinosa-Ruiz, Ana, Fraser, Paul D, Martin, Cathie, Granell, Antonio, Orzáez, Diego
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346294
Acceso en línea:http://hdl.handle.net/10261/346294
https://api.elsevier.com/content/abstract/scopus_id/85172162894
Access Level:acceso abierto
Palabra clave:Cisgenesis
Biofortification
Branched amino acids
Flavonoids
Intragenesis
Tomato
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
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spelling Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids contentVázquez-Vilar, MartaFernandez-Del-Carmen, AsunGarcia-Carpintero, VictorDrapal, MargitPresa, SilviaRicci, DoroteaDiretto, GianfrancoRambla, José LuisFernández-Muñoz, RafaelEspinosa-Ruiz, AnaFraser, Paul DMartin, CathieGranell, AntonioOrzáez, DiegoCisgenesisBiofortificationBranched amino acidsFlavonoidsIntragenesisTomatohttp://metadata.un.org/sdg/12Ensure sustainable consumption and production patternsHigher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation of branched-chain amino acids (BCAAs) in vegan diets can reduce risks associated to their deficiency, particularly in older adults, which can cause loss of skeletal muscle strength and mass. Most plant-derived foods contain only small amounts of BCAAs, and those plants with high levels of flavonoids are not eaten broadly. Here we describe the generation of metabolically engineered cisgenic tomatoes enriched in both flavonoids and BCAAs. In this approach, coding and regulatory DNA elements, all derived from the tomato genome, were combined to obtain a herbicide-resistant version of an acetolactate synthase (mSlALS) gene expressed broadly and a MYB12-like transcription factor (SlMYB12) expressed in a fruit-specific manner. The mSlALS played a dual role, as a selectable marker as well as being key enzyme in BCAA enrichment. The resulting cisgenic tomatoes were highly enriched in Leucine (21-fold compared to wild-type levels), Valine (ninefold) and Isoleucine (threefold) and concomitantly biofortified in several antioxidant flavonoids including kaempferol (64-fold) and quercetin (45-fold). Comprehensive metabolomic and transcriptomic analysis of the biofortified cisgenic tomatoes revealed marked differences to wild type and could serve to evaluate the safety of these biofortified fruits for human consumption.This work has been funded by grant PID2019-108203RB-100 from the Spanish Ministerio de Ciencia e Innovación, through the Agencia Estatal de Investigación (co-financed European Regional Development Fund). MVV acknowledges support by the Generalitat Valenciana and Fondo Social Europeo through a post-doctoral grant (APOSTD/2020/096) and by the European Molecular Biology Organization through a Short-Term Fellowship (ASTF 171-2013). JLR acknowledges support by the Spanish Ministry of Science and Innovation through a Juan de la Cierva-Incorporación grant (IJC2020-045612-I).Peer reviewedWiley-BlackwellMinisterio de Ciencia e Innovación (España)0000-0001-7175-78180000-0001-9586-83280000-0002-1441-02330000-0002-4054-89490000-0002-5953-89000000-0003-1662-5403Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/346294https://api.elsevier.com/content/abstract/scopus_id/85172162894reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108203RB-I00Plant biotechnology journalhttps://doi.org/10.1111/pbi.14163Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3462942026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
title Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
spellingShingle Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
Vázquez-Vilar, Marta
Cisgenesis
Biofortification
Branched amino acids
Flavonoids
Intragenesis
Tomato
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
title_short Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
title_full Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
title_fullStr Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
title_full_unstemmed Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
title_sort Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
dc.creator.none.fl_str_mv Vázquez-Vilar, Marta
Fernandez-Del-Carmen, Asun
Garcia-Carpintero, Victor
Drapal, Margit
Presa, Silvia
Ricci, Dorotea
Diretto, Gianfranco
Rambla, José Luis
Fernández-Muñoz, Rafael
Espinosa-Ruiz, Ana
Fraser, Paul D
Martin, Cathie
Granell, Antonio
Orzáez, Diego
author Vázquez-Vilar, Marta
author_facet Vázquez-Vilar, Marta
Fernandez-Del-Carmen, Asun
Garcia-Carpintero, Victor
Drapal, Margit
Presa, Silvia
Ricci, Dorotea
Diretto, Gianfranco
Rambla, José Luis
Fernández-Muñoz, Rafael
Espinosa-Ruiz, Ana
Fraser, Paul D
Martin, Cathie
Granell, Antonio
Orzáez, Diego
author_role author
author2 Fernandez-Del-Carmen, Asun
Garcia-Carpintero, Victor
Drapal, Margit
Presa, Silvia
Ricci, Dorotea
Diretto, Gianfranco
Rambla, José Luis
Fernández-Muñoz, Rafael
Espinosa-Ruiz, Ana
Fraser, Paul D
Martin, Cathie
Granell, Antonio
Orzáez, Diego
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
0000-0001-7175-7818
0000-0001-9586-8328
0000-0002-1441-0233
0000-0002-4054-8949
0000-0002-5953-8900
0000-0003-1662-5403
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cisgenesis
Biofortification
Branched amino acids
Flavonoids
Intragenesis
Tomato
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
topic Cisgenesis
Biofortification
Branched amino acids
Flavonoids
Intragenesis
Tomato
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
description Higher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation of branched-chain amino acids (BCAAs) in vegan diets can reduce risks associated to their deficiency, particularly in older adults, which can cause loss of skeletal muscle strength and mass. Most plant-derived foods contain only small amounts of BCAAs, and those plants with high levels of flavonoids are not eaten broadly. Here we describe the generation of metabolically engineered cisgenic tomatoes enriched in both flavonoids and BCAAs. In this approach, coding and regulatory DNA elements, all derived from the tomato genome, were combined to obtain a herbicide-resistant version of an acetolactate synthase (mSlALS) gene expressed broadly and a MYB12-like transcription factor (SlMYB12) expressed in a fruit-specific manner. The mSlALS played a dual role, as a selectable marker as well as being key enzyme in BCAA enrichment. The resulting cisgenic tomatoes were highly enriched in Leucine (21-fold compared to wild-type levels), Valine (ninefold) and Isoleucine (threefold) and concomitantly biofortified in several antioxidant flavonoids including kaempferol (64-fold) and quercetin (45-fold). Comprehensive metabolomic and transcriptomic analysis of the biofortified cisgenic tomatoes revealed marked differences to wild type and could serve to evaluate the safety of these biofortified fruits for human consumption.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346294
https://api.elsevier.com/content/abstract/scopus_id/85172162894
url http://hdl.handle.net/10261/346294
https://api.elsevier.com/content/abstract/scopus_id/85172162894
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108203RB-I00
Plant biotechnology journal
https://doi.org/10.1111/pbi.14163
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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