Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools

Animal venoms are complex mixtures of highly specialized toxic molecules. Cnidarians and arachnids produce pore-forming proteins (PFPs) directed against the plasma membrane of their target cells. Among PFPs from cnidarians, actinoporins stand out for their small size and molecular simplicity. While...

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Detalles Bibliográficos
Autores: Rivera de la Torre, Esperanza, Palacios Ortega, Juan, Gavilanes, José G., Martínez Del Pozo, Álvaro, García Linares, Sara
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/13494
Acceso en línea:https://hdl.handle.net/20.500.14352/13494
Access Level:acceso abierto
Palabra clave:577.1
Actinoporin
bioinsecticides
immunotoxin
latrotoxin
pore-forming proteins
transcriptomics
venomics
Bioquímica (Química)
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repository_id_str
spelling Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological ToolsRivera de la Torre, EsperanzaPalacios Ortega, JuanGavilanes, José G.Martínez Del Pozo, ÁlvaroGarcía Linares, Sara577.1Actinoporinbioinsecticidesimmunotoxinlatrotoxinpore-forming proteinstranscriptomicsvenomicsBioquímica (Química)Animal venoms are complex mixtures of highly specialized toxic molecules. Cnidarians and arachnids produce pore-forming proteins (PFPs) directed against the plasma membrane of their target cells. Among PFPs from cnidarians, actinoporins stand out for their small size and molecular simplicity. While native actinoporins require only sphingomyelin for membrane binding, engineered chimeras containing a recognition antibody-derived domain fused to an actinoporin isoform can nonetheless serve as highly specific immunotoxins. Examples of such constructs targeted against malignant cells have been already reported. However, PFPs from arachnid venoms are less well-studied from a structural and functional point of view. Spiders from the Latrodectus genus are professional insect hunters that, as part of their toxic arsenal, produce large PFPs known as latrotoxins. Interestingly, some latrotoxins have been identified as potent and highly-specific insecticides. Given the proteinaceous nature of these toxins, their promising future use as efficient bioinsecticides is discussed throughout this Perspective. Protein engineering and large-scale recombinant production are critical steps for the use of these PFPs as tools to control agriculturally important insect pests. In summary, both families of PFPs, from Cnidaria and Arachnida, appear to be molecules with promising biotechnological applications.MDPIUniversidad Complutense de Madrid20192019-01-0120192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/13494reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/134942026-06-02T12:44:21Z
dc.title.none.fl_str_mv Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
title Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
spellingShingle Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
Rivera de la Torre, Esperanza
577.1
Actinoporin
bioinsecticides
immunotoxin
latrotoxin
pore-forming proteins
transcriptomics
venomics
Bioquímica (Química)
title_short Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
title_full Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
title_fullStr Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
title_full_unstemmed Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
title_sort Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools
dc.creator.none.fl_str_mv Rivera de la Torre, Esperanza
Palacios Ortega, Juan
Gavilanes, José G.
Martínez Del Pozo, Álvaro
García Linares, Sara
author Rivera de la Torre, Esperanza
author_facet Rivera de la Torre, Esperanza
Palacios Ortega, Juan
Gavilanes, José G.
Martínez Del Pozo, Álvaro
García Linares, Sara
author_role author
author2 Palacios Ortega, Juan
Gavilanes, José G.
Martínez Del Pozo, Álvaro
García Linares, Sara
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
Actinoporin
bioinsecticides
immunotoxin
latrotoxin
pore-forming proteins
transcriptomics
venomics
Bioquímica (Química)
topic 577.1
Actinoporin
bioinsecticides
immunotoxin
latrotoxin
pore-forming proteins
transcriptomics
venomics
Bioquímica (Química)
description Animal venoms are complex mixtures of highly specialized toxic molecules. Cnidarians and arachnids produce pore-forming proteins (PFPs) directed against the plasma membrane of their target cells. Among PFPs from cnidarians, actinoporins stand out for their small size and molecular simplicity. While native actinoporins require only sphingomyelin for membrane binding, engineered chimeras containing a recognition antibody-derived domain fused to an actinoporin isoform can nonetheless serve as highly specific immunotoxins. Examples of such constructs targeted against malignant cells have been already reported. However, PFPs from arachnid venoms are less well-studied from a structural and functional point of view. Spiders from the Latrodectus genus are professional insect hunters that, as part of their toxic arsenal, produce large PFPs known as latrotoxins. Interestingly, some latrotoxins have been identified as potent and highly-specific insecticides. Given the proteinaceous nature of these toxins, their promising future use as efficient bioinsecticides is discussed throughout this Perspective. Protein engineering and large-scale recombinant production are critical steps for the use of these PFPs as tools to control agriculturally important insect pests. In summary, both families of PFPs, from Cnidaria and Arachnida, appear to be molecules with promising biotechnological applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/13494
url https://hdl.handle.net/20.500.14352/13494
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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