Folate transport by prawn hepatopancreas brush-border membrane vesicles

The transport system of folic acid (Pte-Glu) by brush-border membrane vesicles (BBMV) isolated from prawn (Penaeus japonicm) hepatopancreas, was studied by measuring the uptake of Pte-Glu. This uptake was found to have two components, intravesicular transport and membrane binding. Membrane binding w...

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Detalles Bibliográficos
Autores: Blaya, J. A., Muriana, Francisco J. G., Ruiz-Gutiérrez, Valentina, Vázquez, Carmen M., Bolufer, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1998
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/247558
Acceso en línea:http://hdl.handle.net/10261/247558
Access Level:acceso abierto
Palabra clave:Folate transport
Prawn
Hepatopancreas
Brush-border membrane vesicles
Kinetics
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spelling Folate transport by prawn hepatopancreas brush-border membrane vesiclesBlaya, J. A.Muriana, Francisco J. G.Ruiz-Gutiérrez, ValentinaVázquez, Carmen M.Bolufer, J.Folate transportPrawnHepatopancreasBrush-border membrane vesiclesKineticsThe transport system of folic acid (Pte-Glu) by brush-border membrane vesicles (BBMV) isolated from prawn (Penaeus japonicm) hepatopancreas, was studied by measuring the uptake of Pte-Glu. This uptake was found to have two components, intravesicular transport and membrane binding. Membrane binding was not affected by the presence of a transmembrane pH-gradient at a short incubation period. However, a transmembrane pH-gradient increased membrane binding at 60 min. The transport of Pte-Glu appeared to be carrier-mediated, was stimulated by an inwardly proton gradient (pH 5.5 outside, 7.4 inside) and was unaffected by a sodium-gradient. The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis–Menten kinetics. Eadie–Hofstee representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.37 μM and Vmax of 1.06 pmol/mg protein/15 s. These findings indicate that BBMV isolated from prawn hepatopancreas possesses a Pte-Glu transport system similar to that described in mammalian intestine.This work was supported by a grant from the Spanish CICYT MAR89-0341.Portland PressBiochemical SocietyComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202119982021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/247558reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1023/A:1022236616277Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2475582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Folate transport by prawn hepatopancreas brush-border membrane vesicles
title Folate transport by prawn hepatopancreas brush-border membrane vesicles
spellingShingle Folate transport by prawn hepatopancreas brush-border membrane vesicles
Blaya, J. A.
Folate transport
Prawn
Hepatopancreas
Brush-border membrane vesicles
Kinetics
title_short Folate transport by prawn hepatopancreas brush-border membrane vesicles
title_full Folate transport by prawn hepatopancreas brush-border membrane vesicles
title_fullStr Folate transport by prawn hepatopancreas brush-border membrane vesicles
title_full_unstemmed Folate transport by prawn hepatopancreas brush-border membrane vesicles
title_sort Folate transport by prawn hepatopancreas brush-border membrane vesicles
dc.creator.none.fl_str_mv Blaya, J. A.
Muriana, Francisco J. G.
Ruiz-Gutiérrez, Valentina
Vázquez, Carmen M.
Bolufer, J.
author Blaya, J. A.
author_facet Blaya, J. A.
Muriana, Francisco J. G.
Ruiz-Gutiérrez, Valentina
Vázquez, Carmen M.
Bolufer, J.
author_role author
author2 Muriana, Francisco J. G.
Ruiz-Gutiérrez, Valentina
Vázquez, Carmen M.
Bolufer, J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Folate transport
Prawn
Hepatopancreas
Brush-border membrane vesicles
Kinetics
topic Folate transport
Prawn
Hepatopancreas
Brush-border membrane vesicles
Kinetics
description The transport system of folic acid (Pte-Glu) by brush-border membrane vesicles (BBMV) isolated from prawn (Penaeus japonicm) hepatopancreas, was studied by measuring the uptake of Pte-Glu. This uptake was found to have two components, intravesicular transport and membrane binding. Membrane binding was not affected by the presence of a transmembrane pH-gradient at a short incubation period. However, a transmembrane pH-gradient increased membrane binding at 60 min. The transport of Pte-Glu appeared to be carrier-mediated, was stimulated by an inwardly proton gradient (pH 5.5 outside, 7.4 inside) and was unaffected by a sodium-gradient. The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis–Menten kinetics. Eadie–Hofstee representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.37 μM and Vmax of 1.06 pmol/mg protein/15 s. These findings indicate that BBMV isolated from prawn hepatopancreas possesses a Pte-Glu transport system similar to that described in mammalian intestine.
publishDate 1998
dc.date.none.fl_str_mv 1998
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/247558
url http://hdl.handle.net/10261/247558
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1023/A:1022236616277
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Portland Press
Biochemical Society
publisher.none.fl_str_mv Portland Press
Biochemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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