Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Polymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanof...

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Autores: Loyo, C., Cordoba, Alexander, Palza, H., Canales, Daniel, Melo, Francisco, Vivanco, Juan F., Baier, R.V., Millán, Carola, Corrales, Teresa, Zapata, Paula A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/341622
Acceso en línea:http://hdl.handle.net/10261/341622
Access Level:acceso abierto
Palabra clave:Electrospinning
Polycaprolactone
Graphene oxide
Gelatin coating degradability
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spelling Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue RegenerationLoyo, C.Cordoba, AlexanderPalza, H.Canales, DanielMelo, FranciscoVivanco, Juan F.Baier, R.V.Millán, CarolaCorrales, TeresaZapata, Paula A.ElectrospinningPolycaprolactoneGraphene oxideGelatin coating degradabilityPolymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanofibrous scaffolds fabricated by an electrospinning technique with 1 and 2 wt% GO. Uniform PCL/GO fibers were obtained with a beadless structure and rough surface. PCL/GO scaffolds exhibited an increase in their crystallization temperature (Tc), attributed to GO, which acted as a nucleation agent. Young’s modulus increased by 32 and 63% for the incorporation of 1 and 2 wt% GO, respectively, in comparison with neat PCL. A homogeneous Gt coating was further applied to these fibers, with incorporations as high as 24.7 wt%. The introduction of the Gt coating improved the hydrophilicity and degradability of the scaffolds. Bioactivity analysis revealed that the hydroxyapatite crystals were deposited on the Gt-coated scaffolds, which made them different from their uncoated counterparts. Our results showed the synergic effect of Gt and GO in enhancing the multifunctionality of the PCL, in particular the degradability rate, bioactivity, and cell adhesion and proliferation of hGMSC cells, making it an interesting biomaterial for BTE.P. Zapata and F. Melo thank Universidad de Santiago de Chile, Usach. Proyecto Dicyt 052241ZR_DAS, Vicerrectoría de Investigación, Desarrollo e Innovación. We also thank ANID Basal funding for the Scientific and Technological Center of Excellence, IMPACT, #FB210024, financed by MICIN (Project Ref. PID2021-124926NB-100). C. Millán and J.F. Vivanco also thank funding from “Agencia Nacional de Investigación y Desarrollo” (ANID) from the Government of Chile through the FONDECYT Nº 1231708 Research Project.Peer reviewedMultidisciplinary Digital Publishing InstituteUniversidad de Santiago de ChileMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Agencia Nacional de Investigación y Desarrollo (Chile)Gobierno de ChileConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/341622reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//PID2021-124926NB-100The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/polym16010129https://doi.org/10.3390/polym16010129Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3416222026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
title Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
spellingShingle Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
Loyo, C.
Electrospinning
Polycaprolactone
Graphene oxide
Gelatin coating degradability
title_short Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
title_full Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
title_fullStr Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
title_full_unstemmed Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
title_sort Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration
dc.creator.none.fl_str_mv Loyo, C.
Cordoba, Alexander
Palza, H.
Canales, Daniel
Melo, Francisco
Vivanco, Juan F.
Baier, R.V.
Millán, Carola
Corrales, Teresa
Zapata, Paula A.
author Loyo, C.
author_facet Loyo, C.
Cordoba, Alexander
Palza, H.
Canales, Daniel
Melo, Francisco
Vivanco, Juan F.
Baier, R.V.
Millán, Carola
Corrales, Teresa
Zapata, Paula A.
author_role author
author2 Cordoba, Alexander
Palza, H.
Canales, Daniel
Melo, Francisco
Vivanco, Juan F.
Baier, R.V.
Millán, Carola
Corrales, Teresa
Zapata, Paula A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Santiago de Chile
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Agencia Nacional de Investigación y Desarrollo (Chile)
Gobierno de Chile
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electrospinning
Polycaprolactone
Graphene oxide
Gelatin coating degradability
topic Electrospinning
Polycaprolactone
Graphene oxide
Gelatin coating degradability
description Polymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanofibrous scaffolds fabricated by an electrospinning technique with 1 and 2 wt% GO. Uniform PCL/GO fibers were obtained with a beadless structure and rough surface. PCL/GO scaffolds exhibited an increase in their crystallization temperature (Tc), attributed to GO, which acted as a nucleation agent. Young’s modulus increased by 32 and 63% for the incorporation of 1 and 2 wt% GO, respectively, in comparison with neat PCL. A homogeneous Gt coating was further applied to these fibers, with incorporations as high as 24.7 wt%. The introduction of the Gt coating improved the hydrophilicity and degradability of the scaffolds. Bioactivity analysis revealed that the hydroxyapatite crystals were deposited on the Gt-coated scaffolds, which made them different from their uncoated counterparts. Our results showed the synergic effect of Gt and GO in enhancing the multifunctionality of the PCL, in particular the degradability rate, bioactivity, and cell adhesion and proliferation of hGMSC cells, making it an interesting biomaterial for BTE.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/341622
url http://hdl.handle.net/10261/341622
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//PID2021-124926NB-100
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/polym16010129
https://doi.org/10.3390/polym16010129
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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