Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells
HEK-293; HLA; Antigen presentation
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Departament de Salut de la Generalitat de Catalunya (DS) |
| Repositorio: | Scientia. Dipòsit d'Informació Digital del Departament de Salut |
| OAI Identifier: | oai:dnet:scientia____::909b3b556d76fd00c856463c5957b4fa |
| Acceso en línea: | http://hdl.handle.net/11351/14361 |
| Access Level: | acceso abierto |
| Palabra clave: | COVID-19 (Malaltia) Pèptids Antígens HLA DISEASES::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections ANATOMY::Cells::Cells, Cultured::Cell Line::Cell Line, Transformed::HEK293 Cells CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class I::HLA-A Antigens::HLA-A3 Antigen CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides ENFERMEDADES::virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por Coronavirus ANATOMÍA::células::células cultivadas::línea celular::línea celular transformada::células HEK293 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::glicoproteínas::glicoproteínas de membranas::antígenos de histocompatibilidad de clase I::antígenos HLA-A::antígeno HLA-A3 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos |
| id |
ES_7bc2d83d0eac15d19cf63b99d12d62ec |
|---|---|
| oai_identifier_str |
oai:dnet:scientia____::909b3b556d76fd00c856463c5957b4fa |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Specific Instability of HLA-A*03:01 Expression in HEK-293 CellsArea-Navarro, MaríaPastor Moreno, AlbaScholz Valero, Erika MargaretCerqueira, AméricoTirado Herranz, Adrián Marcilla, MiguelCanals, FrancescCOVID-19 (Malaltia)PèptidsAntígens HLADISEASES::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus InfectionsANATOMY::Cells::Cells, Cultured::Cell Line::Cell Line, Transformed::HEK293 CellsCHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class I::HLA-A Antigens::HLA-A3 AntigenCHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::PeptidesENFERMEDADES::virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por CoronavirusANATOMÍA::células::células cultivadas::línea celular::línea celular transformada::células HEK293COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::glicoproteínas::glicoproteínas de membranas::antígenos de histocompatibilidad de clase I::antígenos HLA-A::antígeno HLA-A3COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidosHEK-293; HLA; Antigen presentationHEK-293; HLA; Presentación de antígenosHEK-293; HLA; Presentació d'antígensHEK-293 is a highly transfectable human cell line widely used as a model for protein expression. Since large amounts of cells are often required for the purification of HLA immunopeptidomes, suspension-growing variants, such as HEK-293F, facilitate the generation of sufficient cell quantities. The HLA class I-typing of these cells is HLA-A*02:01, -A*03:01, -B*07:02, and -C*07:02. HEK-293T cells have been previously used as a source of HLA peptide ligands derived from SARS-CoV-2 proteins. In this study, we purified and analyzed the HLA-I immunopeptidome of HEK-293 and HEK-293F cells using mass spectrometry. Cell surface expression of specific HLA-I allotypes was determined using flow cytometry with allele-specific antibodies. The HLA-I immunopeptidome of HEK-293 cells contained ligands from all three HLA-I allotypes, whereas that of HEK-293F cells lacked peptides derived from HLA-A*03:01. Flow cytometry experiments confirmed the absence of HLA-A*03:01 expression on the surface of HEK-293F cells. Additionally, we generated a HEK-293 transfectant co-expressing the β5i proteasome subunit and the SARS-CoV-2 Spike protein. This transfectant showed selective loss of HLA-A*03:01 expression, suggesting that HEK-293 linages tend to specifically lose this allotype. We propose that HEK-293F cells are unsuitable for the identification of HLA-A*03:01 ligands or for stimulating T-cell responses restricted to this allele. Moreover, HLA-A*03:01 expression should be regularly monitored in HEK-293-derived cells.This research was funded by Agència de Gestió d’Ajuts universitaris i de Recerca (AGAUR), grant number “2020PANDE00079” and Universitat Autònoma de Barcelona, grant number “PPC2024-38”.MDPIInstitut Català de la Salut[Area-Navarro M, Pastor-Moreno A, Scholz E, Tirado-Herranz A] Department of Cell Biology, Physiology and Immunology, Institute of Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, Spain. [Cerqueira A, Marcilla M] Proteomics Unit, Spanish National Biotechnology Centre, Madrid, Spain. [Canals F] Proteomics Laboratory, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, SpainVall d'Hebron Barcelona Hospital Campus202620262025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/11351/14361Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésInternational Journal of Molecular Sciences;26(23)https://doi.org/10.3390/ijms262311357Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:scientia____::909b3b556d76fd00c856463c5957b4fa2026-06-12T09:38:37Z |
| dc.title.none.fl_str_mv |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| title |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| spellingShingle |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells Area-Navarro, María COVID-19 (Malaltia) Pèptids Antígens HLA DISEASES::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections ANATOMY::Cells::Cells, Cultured::Cell Line::Cell Line, Transformed::HEK293 Cells CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class I::HLA-A Antigens::HLA-A3 Antigen CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides ENFERMEDADES::virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por Coronavirus ANATOMÍA::células::células cultivadas::línea celular::línea celular transformada::células HEK293 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::glicoproteínas::glicoproteínas de membranas::antígenos de histocompatibilidad de clase I::antígenos HLA-A::antígeno HLA-A3 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos |
| title_short |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| title_full |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| title_fullStr |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| title_full_unstemmed |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| title_sort |
Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells |
| dc.creator.none.fl_str_mv |
Area-Navarro, María Pastor Moreno, Alba Scholz Valero, Erika Margaret Cerqueira, Américo Tirado Herranz, Adrián Marcilla, Miguel Canals, Francesc |
| author |
Area-Navarro, María |
| author_facet |
Area-Navarro, María Pastor Moreno, Alba Scholz Valero, Erika Margaret Cerqueira, Américo Tirado Herranz, Adrián Marcilla, Miguel Canals, Francesc |
| author_role |
author |
| author2 |
Pastor Moreno, Alba Scholz Valero, Erika Margaret Cerqueira, Américo Tirado Herranz, Adrián Marcilla, Miguel Canals, Francesc |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Institut Català de la Salut [Area-Navarro M, Pastor-Moreno A, Scholz E, Tirado-Herranz A] Department of Cell Biology, Physiology and Immunology, Institute of Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, Spain. [Cerqueira A, Marcilla M] Proteomics Unit, Spanish National Biotechnology Centre, Madrid, Spain. [Canals F] Proteomics Laboratory, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain Vall d'Hebron Barcelona Hospital Campus |
| dc.subject.none.fl_str_mv |
COVID-19 (Malaltia) Pèptids Antígens HLA DISEASES::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections ANATOMY::Cells::Cells, Cultured::Cell Line::Cell Line, Transformed::HEK293 Cells CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class I::HLA-A Antigens::HLA-A3 Antigen CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides ENFERMEDADES::virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por Coronavirus ANATOMÍA::células::células cultivadas::línea celular::línea celular transformada::células HEK293 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::glicoproteínas::glicoproteínas de membranas::antígenos de histocompatibilidad de clase I::antígenos HLA-A::antígeno HLA-A3 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos |
| topic |
COVID-19 (Malaltia) Pèptids Antígens HLA DISEASES::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections ANATOMY::Cells::Cells, Cultured::Cell Line::Cell Line, Transformed::HEK293 Cells CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class I::HLA-A Antigens::HLA-A3 Antigen CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides ENFERMEDADES::virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por Coronavirus ANATOMÍA::células::células cultivadas::línea celular::línea celular transformada::células HEK293 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::glicoproteínas::glicoproteínas de membranas::antígenos de histocompatibilidad de clase I::antígenos HLA-A::antígeno HLA-A3 COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos |
| description |
HEK-293; HLA; Antigen presentation |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2026 2026 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11351/14361 |
| url |
http://hdl.handle.net/11351/14361 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences;26(23) https://doi.org/10.3390/ijms262311357 |
| dc.rights.none.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
Scientia reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut instname:Departament de Salut de la Generalitat de Catalunya (DS) |
| instname_str |
Departament de Salut de la Generalitat de Catalunya (DS) |
| reponame_str |
Scientia. Dipòsit d'Informació Digital del Departament de Salut |
| collection |
Scientia. Dipòsit d'Informació Digital del Departament de Salut |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869411539342065664 |
| score |
15.811543 |