Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α

Despite the increasing number of organs-on-a-chip that have been developed in the past decade, limited efforts have been made to integrate a sensing system for in situ continual measurements of biomarkers from three-dimensional (3D) tissues. Here, we present a custom-made integrated platform for mus...

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Detalhes bibliográficos
Autores: Ortega, María Alejandra, Fernández Garibay, Xiomara, Castaño, Albert G., De Chiara, Francesco, Hernández Albors, Alejandro, Balaguer Trias, Jordina, Ramon Azcon, Javier
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/137178
Acesso em linha:https://hdl.handle.net/2445/137178
Access Level:acceso abierto
Palavra-chave:Marcadors bioquímics
Cultiu cel·lular
Necrosi
Biochemical markers
Cell culture
Necrosis
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spelling Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-αOrtega, María AlejandraFernández Garibay, XiomaraCastaño, Albert G.De Chiara, FrancescoHernández Albors, AlejandroBalaguer Trias, JordinaRamon Azcon, JavierMarcadors bioquímicsCultiu cel·lularNecrosiBiochemical markersCell cultureNecrosisDespite the increasing number of organs-on-a-chip that have been developed in the past decade, limited efforts have been made to integrate a sensing system for in situ continual measurements of biomarkers from three-dimensional (3D) tissues. Here, we present a custom-made integrated platform for muscle cell stimulation under fluidic conditions connected with a multiplexed high-sensitivity electrochemical sensing system for in situ monitoring. To demonstrate this, we use our system to measure the release levels and release time of interleukin 6 and tumor necrosis factor alpha in vitro by 3D muscle microtissue under electrical and biological stimulations. Our experimental design has enabled us to perform multiple time point measurements using functionalized screen-printed gold electrodes with sensitivity in the ng mL−1 range. This affordable setup is uniquely suited for monitoring factors released by 3D single cell types upon external stimulation for metabolic studies.201920202019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion18 p.application/pdfhttps://hdl.handle.net/2445/137178Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1039/C9LC00285ELab on a Chip, 2019https://doi.org/10.1039/C9LC00285Einfo:eu-repo/grantAgreement/EC/FP7/714317info:eu-repo/grantAgreement/EC/H2020/714317(c) The Royal Society of Chemistry, 2019info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1371782026-05-29T05:05:01Z
dc.title.none.fl_str_mv Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
title Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
spellingShingle Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
Ortega, María Alejandra
Marcadors bioquímics
Cultiu cel·lular
Necrosi
Biochemical markers
Cell culture
Necrosis
title_short Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
title_full Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
title_fullStr Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
title_full_unstemmed Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
title_sort Muscle-on-a-chip with an on-site multiplexed biosensing system for in situ monitoring of secreted IL-6 and TNF-α
dc.creator.none.fl_str_mv Ortega, María Alejandra
Fernández Garibay, Xiomara
Castaño, Albert G.
De Chiara, Francesco
Hernández Albors, Alejandro
Balaguer Trias, Jordina
Ramon Azcon, Javier
author Ortega, María Alejandra
author_facet Ortega, María Alejandra
Fernández Garibay, Xiomara
Castaño, Albert G.
De Chiara, Francesco
Hernández Albors, Alejandro
Balaguer Trias, Jordina
Ramon Azcon, Javier
author_role author
author2 Fernández Garibay, Xiomara
Castaño, Albert G.
De Chiara, Francesco
Hernández Albors, Alejandro
Balaguer Trias, Jordina
Ramon Azcon, Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Marcadors bioquímics
Cultiu cel·lular
Necrosi
Biochemical markers
Cell culture
Necrosis
topic Marcadors bioquímics
Cultiu cel·lular
Necrosi
Biochemical markers
Cell culture
Necrosis
description Despite the increasing number of organs-on-a-chip that have been developed in the past decade, limited efforts have been made to integrate a sensing system for in situ continual measurements of biomarkers from three-dimensional (3D) tissues. Here, we present a custom-made integrated platform for muscle cell stimulation under fluidic conditions connected with a multiplexed high-sensitivity electrochemical sensing system for in situ monitoring. To demonstrate this, we use our system to measure the release levels and release time of interleukin 6 and tumor necrosis factor alpha in vitro by 3D muscle microtissue under electrical and biological stimulations. Our experimental design has enabled us to perform multiple time point measurements using functionalized screen-printed gold electrodes with sensitivity in the ng mL−1 range. This affordable setup is uniquely suited for monitoring factors released by 3D single cell types upon external stimulation for metabolic studies.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/137178
url https://hdl.handle.net/2445/137178
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1039/C9LC00285E
Lab on a Chip, 2019
https://doi.org/10.1039/C9LC00285E
info:eu-repo/grantAgreement/EC/FP7/714317
info:eu-repo/grantAgreement/EC/H2020/714317
dc.rights.none.fl_str_mv (c) The Royal Society of Chemistry, 2019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) The Royal Society of Chemistry, 2019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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