Unravelling the consequences of the bacteriophages in human samples

Bacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the ly...

Descripción completa

Detalles Bibliográficos
Autores: Blanco Picazo, Pedro, Fernández Orth, Dietmar, Brown Jaque, Maryury, Miró, Elisenda, Espinal, Paula, Rodríguez-Rubio, Lorena, Muniesa Pérez, Ma. Teresa, Navarro, Ferran
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176817
Acceso en línea:https://hdl.handle.net/2445/176817
Access Level:acceso abierto
Palabra clave:Bacteriòfags
Microorganismes patògens
Bacteriophages
Pathogenic microorganisms
id ES_7b8ee6b25d1bc7b8ffc0bcfb837a2c6e
oai_identifier_str oai:recercat.cat:2445/176817
network_acronym_str ES
network_name_str España
repository_id_str
spelling Unravelling the consequences of the bacteriophages in human samplesBlanco Picazo, PedroFernández Orth, DietmarBrown Jaque, MaryuryMiró, ElisendaEspinal, PaulaRodríguez-Rubio, LorenaMuniesa Pérez, Ma. TeresaNavarro, FerranBacteriòfagsMicroorganismes patògensBacteriophagesPathogenic microorganismsBacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the lysis of the bacterial host and 2) by the detection of bacterial genes inside the phage capsids that mislead the presence of the bacterial pathogen. To unravel these interferences, human samples (n = 271) were analyzed and infectious phages were observed in 11% of blood culture, 28% of serum, 45% of ascitic fluid, 14% of cerebrospinal fluid and 23% of urine samples. The genetic content of phage particles from a pool of urine and ascitic fluid samples corresponded to bacteriophages infecting different bacterial genera. In addition, many bacterial genes packaged in the phage capsids, including antibiotic resistance genes and 16S rRNA genes, were detected in the viromes. Phage interference can be minimized applying a simple procedure that reduced the content of phages up to 3 logs while maintaining the bacterial load. This method reduced the detection of phage genes avoiding the interference with molecular detection of bacteria and reduced the phage propagation in the cultures, enhancing the recovery of bacteria up to 6 logs.Nature Publishing Group2021202120202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/176817Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-020-63432-7Scientific Reports, 2020, vol. 10, num. 1, p. 6737https://doi.org/10.1038/s41598-020-63432-7cc-by (c) Blanco Picazo, Pedro et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1768172026-05-29T05:05:01Z
dc.title.none.fl_str_mv Unravelling the consequences of the bacteriophages in human samples
title Unravelling the consequences of the bacteriophages in human samples
spellingShingle Unravelling the consequences of the bacteriophages in human samples
Blanco Picazo, Pedro
Bacteriòfags
Microorganismes patògens
Bacteriophages
Pathogenic microorganisms
title_short Unravelling the consequences of the bacteriophages in human samples
title_full Unravelling the consequences of the bacteriophages in human samples
title_fullStr Unravelling the consequences of the bacteriophages in human samples
title_full_unstemmed Unravelling the consequences of the bacteriophages in human samples
title_sort Unravelling the consequences of the bacteriophages in human samples
dc.creator.none.fl_str_mv Blanco Picazo, Pedro
Fernández Orth, Dietmar
Brown Jaque, Maryury
Miró, Elisenda
Espinal, Paula
Rodríguez-Rubio, Lorena
Muniesa Pérez, Ma. Teresa
Navarro, Ferran
author Blanco Picazo, Pedro
author_facet Blanco Picazo, Pedro
Fernández Orth, Dietmar
Brown Jaque, Maryury
Miró, Elisenda
Espinal, Paula
Rodríguez-Rubio, Lorena
Muniesa Pérez, Ma. Teresa
Navarro, Ferran
author_role author
author2 Fernández Orth, Dietmar
Brown Jaque, Maryury
Miró, Elisenda
Espinal, Paula
Rodríguez-Rubio, Lorena
Muniesa Pérez, Ma. Teresa
Navarro, Ferran
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriòfags
Microorganismes patògens
Bacteriophages
Pathogenic microorganisms
topic Bacteriòfags
Microorganismes patògens
Bacteriophages
Pathogenic microorganisms
description Bacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the lysis of the bacterial host and 2) by the detection of bacterial genes inside the phage capsids that mislead the presence of the bacterial pathogen. To unravel these interferences, human samples (n = 271) were analyzed and infectious phages were observed in 11% of blood culture, 28% of serum, 45% of ascitic fluid, 14% of cerebrospinal fluid and 23% of urine samples. The genetic content of phage particles from a pool of urine and ascitic fluid samples corresponded to bacteriophages infecting different bacterial genera. In addition, many bacterial genes packaged in the phage capsids, including antibiotic resistance genes and 16S rRNA genes, were detected in the viromes. Phage interference can be minimized applying a simple procedure that reduced the content of phages up to 3 logs while maintaining the bacterial load. This method reduced the detection of phage genes avoiding the interference with molecular detection of bacteria and reduced the phage propagation in the cultures, enhancing the recovery of bacteria up to 6 logs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176817
url https://hdl.handle.net/2445/176817
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-63432-7
Scientific Reports, 2020, vol. 10, num. 1, p. 6737
https://doi.org/10.1038/s41598-020-63432-7
dc.rights.none.fl_str_mv cc-by (c) Blanco Picazo, Pedro et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Blanco Picazo, Pedro et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411523933241344
score 15.81155

Similares en LA Referencia