Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by...

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Authors: Oliveira, Flavio, Díez-Quijada Jiménez, Leticia, Turkina, Maria V., Moraes, Joao-Paulo, Jos Gallego, Ángeles Mencía, Cameán Fernández, Ana María, Barreiro, Aldo, Azevedo, Joana, Vasconcelos, Vitor, Martins, José Carlos, Campos, Alexandre
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/95009
Online Access:https://hdl.handle.net/11441/95009
https://doi.org/10.3390/toxins12030196
Access Level:Open access
Keyword:Mytilus galloprovincialis
toxic cyanobacteria
microcystin
cylindrospermopsin
ecotoxicology
shotgun proteomics
id ES_7b8d432375632378177533b2dfd459f9
oai_identifier_str oai:idus.us.es:11441/95009
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
title Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
spellingShingle Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
Oliveira, Flavio
Mytilus galloprovincialis
toxic cyanobacteria
microcystin
cylindrospermopsin
ecotoxicology
shotgun proteomics
title_short Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
title_full Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
title_fullStr Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
title_full_unstemmed Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
title_sort Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum
dc.creator.none.fl_str_mv Oliveira, Flavio
Díez-Quijada Jiménez, Leticia
Turkina, Maria V.
Moraes, Joao-Paulo
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
Barreiro, Aldo
Azevedo, Joana
Vasconcelos, Vitor
Martins, José Carlos
Campos, Alexandre
author Oliveira, Flavio
author_facet Oliveira, Flavio
Díez-Quijada Jiménez, Leticia
Turkina, Maria V.
Moraes, Joao-Paulo
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
Barreiro, Aldo
Azevedo, Joana
Vasconcelos, Vitor
Martins, José Carlos
Campos, Alexandre
author_role author
author2 Díez-Quijada Jiménez, Leticia
Turkina, Maria V.
Moraes, Joao-Paulo
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
Barreiro, Aldo
Azevedo, Joana
Vasconcelos, Vitor
Martins, José Carlos
Campos, Alexandre
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
Fundação para a Ciência e a Tecnologia. Portugal
NORTE 2020
PORTUGAL 2020
EUROPEAN UNION, European Regional Development Fund (ERDF)
Ministerio de Economia, Industria y Competitividad (MINECO). España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
dc.subject.none.fl_str_mv Mytilus galloprovincialis
toxic cyanobacteria
microcystin
cylindrospermopsin
ecotoxicology
shotgun proteomics
topic Mytilus galloprovincialis
toxic cyanobacteria
microcystin
cylindrospermopsin
ecotoxicology
shotgun proteomics
description Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/95009
https://doi.org/10.3390/toxins12030196
url https://hdl.handle.net/11441/95009
https://doi.org/10.3390/toxins12030196
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Toxins, 12 (3), 196.
(PTDC/ASP-PES/31762/2017) and UID/Multi/04423/2013
Project AGL2015-64558-R
(BES-2016–078773)
https://doi.org/10.3390/toxins12030196
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporumOliveira, FlavioDíez-Quijada Jiménez, LeticiaTurkina, Maria V.Moraes, Joao-PauloJos Gallego, Ángeles MencíaCameán Fernández, Ana MaríaBarreiro, AldoAzevedo, JoanaVasconcelos, VitorMartins, José CarlosCampos, AlexandreMytilus galloprovincialistoxic cyanobacteriamicrocystincylindrospermopsinecotoxicologyshotgun proteomicsToxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYNPortuguese Science Foundation and under the Projects MOREBIVALVES (PTDC/ASP-PES/31762/2017) and UID/Multi/04423/2013NORTE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT. Moreover, Project AGL2015-64558-RMINECO/FEDER, UE, and the grant FPI (BES-2016–078773)MDPINutrición y Bromatología, Toxicología y Medicina LegalFundação para a Ciência e a Tecnologia. PortugalNORTE 2020PORTUGAL 2020EUROPEAN UNION, European Regional Development Fund (ERDF)Ministerio de Economia, Industria y Competitividad (MINECO). EspañaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/95009https://doi.org/10.3390/toxins12030196reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésToxins, 12 (3), 196.(PTDC/ASP-PES/31762/2017) and UID/Multi/04423/2013Project AGL2015-64558-R(BES-2016–078773)https://doi.org/10.3390/toxins12030196info:eu-repo/semantics/openAccessoai:idus.us.es:11441/950092026-06-17T12:51:07Z
score 15.301603

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