Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification

10 pags., 5 figs., 2 tabs.

Detalles Bibliográficos
Autores: Yang, Junhuan, Khan, M.A.K., López-García, Sergio, Nosheen, S., Nazir, Y., Zhang, H., Garre, Victoriano, Song, Y.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/221149
Acceso en línea:http://hdl.handle.net/10261/221149
Access Level:acceso abierto
Palabra clave:Fatty acid desaturase 3
SDA production
Homologous overexpression
Mucor circinelloides
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spelling Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modificationYang, JunhuanKhan, M.A.K.López-García, SergioNosheen, S.Nazir, Y.Zhang, H.Garre, VictorianoSong, Y.Fatty acid desaturase 3SDA productionHomologous overexpressionMucor circinelloides10 pags., 5 figs., 2 tabs.Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.This work was supported by the National Natural Science Foundation of China (Grant Nos. 31670064 and 31972851), Natural Science Foundation of Shandong province (ZR2018BC058) Science and Technology Project of Shandong College (J16LE20 and J17KA133), Taishan Industry Leading Talent Project and starting grant from Shandong University of Technology.Science publishingNational Natural Science Foundation of ChinaNatural Science Foundation of Shandong ProvinceShandong UniversityConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/221149reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3844/ajbbsp.2020.138.147Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2211492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
title Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
spellingShingle Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
Yang, Junhuan
Fatty acid desaturase 3
SDA production
Homologous overexpression
Mucor circinelloides
title_short Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
title_full Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
title_fullStr Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
title_full_unstemmed Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
title_sort Improved SDA production in high lipid accumulating strain of mucor circinelloides WJ11 by genetic modification
dc.creator.none.fl_str_mv Yang, Junhuan
Khan, M.A.K.
López-García, Sergio
Nosheen, S.
Nazir, Y.
Zhang, H.
Garre, Victoriano
Song, Y.
author Yang, Junhuan
author_facet Yang, Junhuan
Khan, M.A.K.
López-García, Sergio
Nosheen, S.
Nazir, Y.
Zhang, H.
Garre, Victoriano
Song, Y.
author_role author
author2 Khan, M.A.K.
López-García, Sergio
Nosheen, S.
Nazir, Y.
Zhang, H.
Garre, Victoriano
Song, Y.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Natural Science Foundation of Shandong Province
Shandong University
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fatty acid desaturase 3
SDA production
Homologous overexpression
Mucor circinelloides
topic Fatty acid desaturase 3
SDA production
Homologous overexpression
Mucor circinelloides
description 10 pags., 5 figs., 2 tabs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/221149
url http://hdl.handle.net/10261/221149
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3844/ajbbsp.2020.138.147
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Science publishing
publisher.none.fl_str_mv Science publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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