The toxicity and intraspecific variability of Alexandrium andersonii Balech

13 páginas, 8 tablas, 6 figuras

Detalles Bibliográficos
Autores: Sampedro, Nagore, Franco, José Mariano, Zapata, Manuel, Riobó, Pilar, Garcés, Esther, Penna, Antonella, Caillaud, Amandine, Diogène, Jorge, Cacho, Emiliano, Camp, Jordi
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/72739
Acceso en línea:http://hdl.handle.net/10261/72739
Access Level:acceso abierto
Palabra clave:Alexandrium andersonii
Cytotoxicity assays
Intraspecific variability
Mediterranean Sea
PSP
Toxicity
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spelling The toxicity and intraspecific variability of Alexandrium andersonii BalechSampedro, NagoreFranco, José MarianoZapata, ManuelRiobó, PilarGarcés, EstherPenna, AntonellaCaillaud, AmandineDiogène, JorgeCacho, EmilianoCamp, JordiAlexandrium andersoniiCytotoxicity assaysIntraspecific variabilityMediterranean SeaPSPToxicity13 páginas, 8 tablas, 6 figurasThe toxicity of Alexandrium andersonii Balech is unclear and its intraspecific variability has yet to be studied. To address these gaps in our knowledge, in the present work five strains of A. andersonii from four different localities were characterized. The results showed that despite genetic homogeneity in the 5.8-ITS (internal transcribed spacer) and large subunit (LSU) regions and similar growth rates, strains originating from different locations varied with respect to cell size, the ratios of certain pigments, and their growth patterns. Cultures of the strains grown at 20 °C were analyzed for toxicity using four different methodologies. The two officially established methods, mouse bioassay and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and post-column reaction analysis of PSP toxins, failed to show the toxicity of any strain. Strains grown at 14 °C were also negative for PSP toxins by HPLC-FLD. However, strains grown at 20 °C exhibited both a response characteristic of the presence of toxin-inhibiting voltage-gated sodium channels, as demonstrated in a neuroblastoma neuro-2a cell-based assay, as well as hemolytic activity in a sheep red blood cell assay.Financial support was provided by the Agència Catalana de l’Aigua (Department de Medi Ambient, Generalitat de Catalunya) and the CSIC through the contract “Pla de vigilància de fitoplàncton nociu i tòxic a la Costa Catalana;” by the “Xarxa de Referència de Recerca i Desenvolupament en Aqüicultura”; project JACUMAR/2007/SANIDAD ANIMAL/PSP2; and project PRIN 2009, financed by the Italian Ministry of Education, University and ResearchPeer reviewedElsevier201320132013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/72739reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.hal.2013.02.003info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/727392026-05-22T06:33:51Z
dc.title.none.fl_str_mv The toxicity and intraspecific variability of Alexandrium andersonii Balech
title The toxicity and intraspecific variability of Alexandrium andersonii Balech
spellingShingle The toxicity and intraspecific variability of Alexandrium andersonii Balech
Sampedro, Nagore
Alexandrium andersonii
Cytotoxicity assays
Intraspecific variability
Mediterranean Sea
PSP
Toxicity
title_short The toxicity and intraspecific variability of Alexandrium andersonii Balech
title_full The toxicity and intraspecific variability of Alexandrium andersonii Balech
title_fullStr The toxicity and intraspecific variability of Alexandrium andersonii Balech
title_full_unstemmed The toxicity and intraspecific variability of Alexandrium andersonii Balech
title_sort The toxicity and intraspecific variability of Alexandrium andersonii Balech
dc.creator.none.fl_str_mv Sampedro, Nagore
Franco, José Mariano
Zapata, Manuel
Riobó, Pilar
Garcés, Esther
Penna, Antonella
Caillaud, Amandine
Diogène, Jorge
Cacho, Emiliano
Camp, Jordi
author Sampedro, Nagore
author_facet Sampedro, Nagore
Franco, José Mariano
Zapata, Manuel
Riobó, Pilar
Garcés, Esther
Penna, Antonella
Caillaud, Amandine
Diogène, Jorge
Cacho, Emiliano
Camp, Jordi
author_role author
author2 Franco, José Mariano
Zapata, Manuel
Riobó, Pilar
Garcés, Esther
Penna, Antonella
Caillaud, Amandine
Diogène, Jorge
Cacho, Emiliano
Camp, Jordi
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alexandrium andersonii
Cytotoxicity assays
Intraspecific variability
Mediterranean Sea
PSP
Toxicity
topic Alexandrium andersonii
Cytotoxicity assays
Intraspecific variability
Mediterranean Sea
PSP
Toxicity
description 13 páginas, 8 tablas, 6 figuras
publishDate 2013
dc.date.none.fl_str_mv 2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/72739
url http://hdl.handle.net/10261/72739
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.hal.2013.02.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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