Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates

Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated f...

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Autores: Brown-Jaque, Maryury, Calero-Cáceres, William, Espinal, Paula|||0000-0002-1014-8936, Rodríguez-Navarro, Judith|||0000-0002-2855-254X, Miró, Elisenda|||0000-0003-2781-6926, González-López, Juanjo|||0000-0003-2419-5909, Cornejo-Sánchez, Thais|||0000-0002-1350-0413, Hurtado, Juan Carlos, Navarro Risueño, Ferran|||0000-0002-4302-2838, Muniesa, Maite|||0000-0001-5549-1394
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:285188
Acceso en línea:https://ddd.uab.cat/record/285188
https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014
Access Level:acceso abierto
Palabra clave:Antibiotic resistance
Bacteriophage
Faeces
Horizontal gene transfer
Transduction
SDG 3 - Good Health and Well-being
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spelling Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolatesBrown-Jaque, MaryuryCalero-Cáceres, WilliamEspinal, Paula|||0000-0002-1014-8936Rodríguez-Navarro, Judith|||0000-0002-2855-254XMiró, Elisenda|||0000-0003-2781-6926González-López, Juanjo|||0000-0003-2419-5909Cornejo-Sánchez, Thais|||0000-0002-1350-0413Hurtado, Juan CarlosNavarro Risueño, Ferran|||0000-0002-4302-2838Muniesa, Maite|||0000-0001-5549-1394Antibiotic resistanceBacteriophageFaecesHorizontal gene transferTransductionSDG 3 - Good Health and Well-beingPhage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with blaTEM and blaCTX-M-9 group being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with blaTEM, blaCTX-M-1 group, blaCTX-M-9 group and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/285188https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD12/0015Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/00329Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CD15/00017Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-0073open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2851882026-06-06T12:50:31Z
dc.title.none.fl_str_mv Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
title Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
spellingShingle Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
Brown-Jaque, Maryury
Antibiotic resistance
Bacteriophage
Faeces
Horizontal gene transfer
Transduction
SDG 3 - Good Health and Well-being
title_short Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
title_full Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
title_fullStr Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
title_full_unstemmed Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
title_sort Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
dc.creator.none.fl_str_mv Brown-Jaque, Maryury
Calero-Cáceres, William
Espinal, Paula|||0000-0002-1014-8936
Rodríguez-Navarro, Judith|||0000-0002-2855-254X
Miró, Elisenda|||0000-0003-2781-6926
González-López, Juanjo|||0000-0003-2419-5909
Cornejo-Sánchez, Thais|||0000-0002-1350-0413
Hurtado, Juan Carlos
Navarro Risueño, Ferran|||0000-0002-4302-2838
Muniesa, Maite|||0000-0001-5549-1394
author Brown-Jaque, Maryury
author_facet Brown-Jaque, Maryury
Calero-Cáceres, William
Espinal, Paula|||0000-0002-1014-8936
Rodríguez-Navarro, Judith|||0000-0002-2855-254X
Miró, Elisenda|||0000-0003-2781-6926
González-López, Juanjo|||0000-0003-2419-5909
Cornejo-Sánchez, Thais|||0000-0002-1350-0413
Hurtado, Juan Carlos
Navarro Risueño, Ferran|||0000-0002-4302-2838
Muniesa, Maite|||0000-0001-5549-1394
author_role author
author2 Calero-Cáceres, William
Espinal, Paula|||0000-0002-1014-8936
Rodríguez-Navarro, Judith|||0000-0002-2855-254X
Miró, Elisenda|||0000-0003-2781-6926
González-López, Juanjo|||0000-0003-2419-5909
Cornejo-Sánchez, Thais|||0000-0002-1350-0413
Hurtado, Juan Carlos
Navarro Risueño, Ferran|||0000-0002-4302-2838
Muniesa, Maite|||0000-0001-5549-1394
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antibiotic resistance
Bacteriophage
Faeces
Horizontal gene transfer
Transduction
SDG 3 - Good Health and Well-being
topic Antibiotic resistance
Bacteriophage
Faeces
Horizontal gene transfer
Transduction
SDG 3 - Good Health and Well-being
description Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with blaTEM and blaCTX-M-9 group being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with blaTEM, blaCTX-M-1 group, blaCTX-M-9 group and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/285188
https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014
url https://ddd.uab.cat/record/285188
https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD12/0015
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/00329
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CD15/00017
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-0073
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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