Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus)
Leishmaniosis, caused by phlebotomine sand fly-borne Leishmania spp., is a multi-host and zoonotic disease in tropical, subtropical and temperate climates, worldwide. Leishmania infantum is the only endemic pathogenic species in Europe, and exposure to this protozoan has previously been reported in...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/392400 |
| Acceso en línea: | http://hdl.handle.net/10261/392400 |
| Access Level: | acceso abierto |
| Palabra clave: | Iberian lynx Zoonosis IFAT QPCR Leishmaniosis Risk factor |
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Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| title |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| spellingShingle |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) Barbero-Moyano, Jesús Iberian lynx Zoonosis IFAT QPCR Leishmaniosis Risk factor |
| title_short |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| title_full |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| title_fullStr |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| title_full_unstemmed |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| title_sort |
Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus) |
| dc.creator.none.fl_str_mv |
Barbero-Moyano, Jesús Caballero-Gómez, Javier Gonzálvez, Moisés Martínez, Remigio Moreno, Inmaculada Berriatua, Eduardo Sánchez-Sánchez, Marta Paniagua, Jorge Fernández-Verón, Isabel Rey-Wamba, María Teresa del Crespo, Elena Montoya-Oliver, Ignacio Salcedo, Javier Risalde, María Ángeles García-Bocanegra, Ignacio |
| author |
Barbero-Moyano, Jesús |
| author_facet |
Barbero-Moyano, Jesús Caballero-Gómez, Javier Gonzálvez, Moisés Martínez, Remigio Moreno, Inmaculada Berriatua, Eduardo Sánchez-Sánchez, Marta Paniagua, Jorge Fernández-Verón, Isabel Rey-Wamba, María Teresa del Crespo, Elena Montoya-Oliver, Ignacio Salcedo, Javier Risalde, María Ángeles García-Bocanegra, Ignacio |
| author_role |
author |
| author2 |
Caballero-Gómez, Javier Gonzálvez, Moisés Martínez, Remigio Moreno, Inmaculada Berriatua, Eduardo Sánchez-Sánchez, Marta Paniagua, Jorge Fernández-Verón, Isabel Rey-Wamba, María Teresa del Crespo, Elena Montoya-Oliver, Ignacio Salcedo, Javier Risalde, María Ángeles García-Bocanegra, Ignacio |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
European Commission Instituto de Salud Carlos III Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) Junta de Andalucía Universidad de Córdoba (España) Junta de Comunidades de Castilla-La Mancha Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Iberian lynx Zoonosis IFAT QPCR Leishmaniosis Risk factor |
| topic |
Iberian lynx Zoonosis IFAT QPCR Leishmaniosis Risk factor |
| description |
Leishmaniosis, caused by phlebotomine sand fly-borne Leishmania spp., is a multi-host and zoonotic disease in tropical, subtropical and temperate climates, worldwide. Leishmania infantum is the only endemic pathogenic species in Europe, and exposure to this protozoan has previously been reported in a wide range of wild mammals, including felids. However, the information about the exposure of the threatened Iberian lynx (Lynx pardinus) to L. infantum is still very scarce. Consequently, the aims of the present large-scale epidemiological study were: (1) to evaluate the circulation of L. infantum in the free-ranging and captive Iberian lynx populations and (2) to investigate potential risk factors associated with L. infantum exposure during the study period, between 2010 and 2022. A total of 783 Iberian lynxes from captive (n = 296) and free-ranging (n = 487) populations were sampled from different areas of the Iberian Peninsula. Exposure to L. infantum (positive to indirect fluorescent antibody test (IFAT) in serum and/or real-time quantitative PCR (qPCR) in spleen samples) was detected in 80 of the 783 lynxes, so the estimated prevalence (95 % confidence limits) was 10.2 % (8.1–12.3 %). Specifically, anti-Leishmania antibodies were found in 12 of the 469 lynxes (2.6 %; 1.1–4.0 %) and L. infantum kDNA was detected in 68 of the 333 lynxes (20.4 %; 16.1–24.8 %) with a Ct range from 26.0 to 38.0 (median: 36.0). One seroconversion and one seroreversion were observed among the 42 Iberian lynxes longitudinally sampled. Phylogenetic analysis revealed high homology (99.9–100 %) with other L. infantum sequences obtained from wild rabbits (Oryctolagus cuniculus), Iberian hares (Lepus granatensis) and humans from Spain. The multivariate analysis identified the habitat status (free-ranging) and age (adult and subadult) as risk factors potentially associated with L. infantum exposure in Iberian lynxes. Our results indicate moderate and widespread circulation of this pathogen in the Iberian lynx populations, suggesting its potential role as spillover hosts in Iberian Mediterranean ecosystems. Additional studies are warranted to determine the clinical impact of L. infantum exposure in this threatened species. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025 2025 |
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info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
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article |
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acceptedVersion |
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http://hdl.handle.net/10261/392400 |
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http://hdl.handle.net/10261/392400 |
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Inglés |
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Inglés |
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The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.prevetmed.2024.106366 https://doi.org/10.1016/j.prevetmed.2024.106366 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869411500004737024 |
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Monitoring of Leishmania infantum exposure in the Iberian lynx (Lynx pardinus)Barbero-Moyano, JesúsCaballero-Gómez, JavierGonzálvez, MoisésMartínez, RemigioMoreno, InmaculadaBerriatua, EduardoSánchez-Sánchez, MartaPaniagua, JorgeFernández-Verón, IsabelRey-Wamba, María Teresa delCrespo, ElenaMontoya-Oliver, IgnacioSalcedo, JavierRisalde, María ÁngelesGarcía-Bocanegra, IgnacioIberian lynxZoonosisIFATQPCRLeishmaniosisRisk factorLeishmaniosis, caused by phlebotomine sand fly-borne Leishmania spp., is a multi-host and zoonotic disease in tropical, subtropical and temperate climates, worldwide. Leishmania infantum is the only endemic pathogenic species in Europe, and exposure to this protozoan has previously been reported in a wide range of wild mammals, including felids. However, the information about the exposure of the threatened Iberian lynx (Lynx pardinus) to L. infantum is still very scarce. Consequently, the aims of the present large-scale epidemiological study were: (1) to evaluate the circulation of L. infantum in the free-ranging and captive Iberian lynx populations and (2) to investigate potential risk factors associated with L. infantum exposure during the study period, between 2010 and 2022. A total of 783 Iberian lynxes from captive (n = 296) and free-ranging (n = 487) populations were sampled from different areas of the Iberian Peninsula. Exposure to L. infantum (positive to indirect fluorescent antibody test (IFAT) in serum and/or real-time quantitative PCR (qPCR) in spleen samples) was detected in 80 of the 783 lynxes, so the estimated prevalence (95 % confidence limits) was 10.2 % (8.1–12.3 %). Specifically, anti-Leishmania antibodies were found in 12 of the 469 lynxes (2.6 %; 1.1–4.0 %) and L. infantum kDNA was detected in 68 of the 333 lynxes (20.4 %; 16.1–24.8 %) with a Ct range from 26.0 to 38.0 (median: 36.0). One seroconversion and one seroreversion were observed among the 42 Iberian lynxes longitudinally sampled. Phylogenetic analysis revealed high homology (99.9–100 %) with other L. infantum sequences obtained from wild rabbits (Oryctolagus cuniculus), Iberian hares (Lepus granatensis) and humans from Spain. The multivariate analysis identified the habitat status (free-ranging) and age (adult and subadult) as risk factors potentially associated with L. infantum exposure in Iberian lynxes. Our results indicate moderate and widespread circulation of this pathogen in the Iberian lynx populations, suggesting its potential role as spillover hosts in Iberian Mediterranean ecosystems. Additional studies are warranted to determine the clinical impact of L. infantum exposure in this threatened species.This article is based upon work from project LIFE 19NAT/ES001055 LYNXCONNECT ‘Creating a genetically and demographically functional Iberian Lynx (Lynx pardinus) metapopulation (2020–2025)’ supported by the European Commission. This research was also partially supported by the CIBER -Consorcio Centro de Investigación Biomédica en Red- (CB 2021/13/00083), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea – NextGenerationEU. J. Barbero-Moyano was supported by an FPU grant from the Spanish Ministry of Science, Innovation and Universities (FPU20/00180). J. Caballero-Gómez was supported by the CIBER -Consorcio Centro de Investigación Biomédica en Red-(CB21/13/00083), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea-Next Generation EU. M. Gonzálvez was supported by a postdoctoral contract Margarita Salas (University of Murcia) from the Program of Requalification of the Spanish University System (Spanish Ministry of Universities) financed by the European Union-NextGenerationEU. R. Martínez was supported by a postdoctoral contract from the “Consejería de Transformación Económica, Industria, Conocimiento y Universidades” of the Regional Government of Andalusia and the University of Córdoba (05yc77b46). M. Sánchez-Sánchez was supported by Investigo Program, funded by European Union (Next Generation EU). We thank all the veterinarians and animal keepers of ex situ and in situ conservation programs involved in the sampling as well as all the members of the CAD centre for their assistance in the collection of samples and epidemiological information. The authors would like to thank Salvador Rejón for their excellent technical support in sample processing and analysis. We also gratefully acknowledge Junta de Andalucía and Junta de Comunidades de Castilla-La Mancha.Peer reviewedElsevierEuropean CommissionInstituto de Salud Carlos IIIAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Junta de AndalucíaUniversidad de Córdoba (España)Junta de Comunidades de Castilla-La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/392400reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.prevetmed.2024.106366https://doi.org/10.1016/j.prevetmed.2024.106366Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3924002026-05-22T06:33:51Z |
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15.81155 |