The class I bHLH factors E2-2A and E2-2B regulate EMT

11 pages, 6 figures.

Detalles Bibliográficos
Autores: Sobrado, Verónica R., Moreno-Bueno, Gema, Cubillo, Eva, Holt, Liam J., Nieto, M. Ángela, Portillo, Francisco, Cano, Amparo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24910
Acceso en línea:http://hdl.handle.net/10261/24910
Access Level:acceso abierto
Palabra clave:Bhlh E2-2
E-cadherin repression
Emt
E47
Snail
Tumour progression
Gene profiling
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spelling The class I bHLH factors E2-2A and E2-2B regulate EMTSobrado, Verónica R.Moreno-Bueno, GemaCubillo, EvaHolt, Liam J.Nieto, M. ÁngelaPortillo, FranciscoCano, AmparoBhlh E2-2E-cadherin repressionEmtE47SnailTumour progressionGene profiling11 pages, 6 figures.Functional loss of the cell-cell adhesion molecule E-cadherin is an essential event for epithelial-mesenchymal transition (EMT), a process that allows cell migration during embryonic development and tumour invasion. In most carcinomas, transcriptional repression has emerged as the main mechanism responsible for E-cadherin downregulation. Here, we report the identification of class I bHLH factor E2-2 (TCF4/ITF2) as a new EMT regulator. Both isoforms of E2-2 (E2-2A and E2-2B) induce a full EMT when overexpressed in MDCK cells but without affecting the tumorigenic properties of parental cells, in contrast to other EMT inducers, such as Snail1 or class I bHLH E47. E-cadherin repression mediated by E2-2 is indirect and independent of proximal E-boxes of the promoter. Knockdown studies indicate that E2-2 expression is dispensable for maintenance of the EMT driven by Snail1 and E47. Comparative gene-profiling analysis reveals that E2-2 factors induce similar, yet distinct, genetic programs to that induced by E47 in MDCK cells. These results, together with the embryonic expression pattern of Tcf4 and E2A (which encodes E12/E47), support a distinct role for E2-2 and suggest an interesting interplay between E-cadherin repressors in the regulation of physiological and pathological EMT processes.This work was supported by the Spanish Ministry of Education and Science (SAF2004-00361; SAF2007-63051) and the EV (MRTN 2004-005428) to A.C., Consolider-Ingenio 2010 CDC2007-00017 to A.C. and M.A.N., and the EU (MRTN 2004-005428) to A.C. and by the Fundación Mutua Madrileña (to G.M.B.). V.R.S. was supported by an FPU fellowship from the Spanish Ministry of Education and Science. G.M.B. is a junior investigator of the Ramón y Cajal Program 2004.Peer reviewedCompany of BiologistsMinisterio de Educación y Ciencia (España)Fundación Mutua MadrileñaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201020102009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersion2960820 bytesapplication/pdfhttp://hdl.handle.net/10261/24910reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jcs.028241Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/249102026-05-22T06:33:51Z
dc.title.none.fl_str_mv The class I bHLH factors E2-2A and E2-2B regulate EMT
title The class I bHLH factors E2-2A and E2-2B regulate EMT
spellingShingle The class I bHLH factors E2-2A and E2-2B regulate EMT
Sobrado, Verónica R.
Bhlh E2-2
E-cadherin repression
Emt
E47
Snail
Tumour progression
Gene profiling
title_short The class I bHLH factors E2-2A and E2-2B regulate EMT
title_full The class I bHLH factors E2-2A and E2-2B regulate EMT
title_fullStr The class I bHLH factors E2-2A and E2-2B regulate EMT
title_full_unstemmed The class I bHLH factors E2-2A and E2-2B regulate EMT
title_sort The class I bHLH factors E2-2A and E2-2B regulate EMT
dc.creator.none.fl_str_mv Sobrado, Verónica R.
Moreno-Bueno, Gema
Cubillo, Eva
Holt, Liam J.
Nieto, M. Ángela
Portillo, Francisco
Cano, Amparo
author Sobrado, Verónica R.
author_facet Sobrado, Verónica R.
Moreno-Bueno, Gema
Cubillo, Eva
Holt, Liam J.
Nieto, M. Ángela
Portillo, Francisco
Cano, Amparo
author_role author
author2 Moreno-Bueno, Gema
Cubillo, Eva
Holt, Liam J.
Nieto, M. Ángela
Portillo, Francisco
Cano, Amparo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Fundación Mutua Madrileña
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bhlh E2-2
E-cadherin repression
Emt
E47
Snail
Tumour progression
Gene profiling
topic Bhlh E2-2
E-cadherin repression
Emt
E47
Snail
Tumour progression
Gene profiling
description 11 pages, 6 figures.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24910
url http://hdl.handle.net/10261/24910
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jcs.028241
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2960820 bytes
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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