Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)

Plastic pollution is a growing concern, especially poly(ethylene terephthalate) (PET), one of the most produced plastic polymers. Although several microorganisms capable of degrading PET have been identified, little is known about those present in wastewater treatment plants (WWTPs). This study expl...

Descripción completa

Detalles Bibliográficos
Autores: Vijande, Carlota, Balboa Méndez, Sabela, Lazzari, Massimo, Lema Rodicio, Juan Manuel, Pabst, Martin
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:dnet:minerva_____::d83d797afd10eea376d7e53001453a83
Acceso en línea:https://hdl.handle.net/10347/46844
Access Level:acceso abierto
Palabra clave:Biofilm
Metaproteomics
Metagenomics
PET biodegradation
Investigación
id ES_7b125b21e3dcf5a10315fecf4698a2eb
oai_identifier_str oai:dnet:minerva_____::d83d797afd10eea376d7e53001453a83
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)Vijande, CarlotaBalboa Méndez, SabelaLazzari, MassimoLema Rodicio, Juan ManuelPabst, MartinBiofilmMetaproteomicsMetagenomicsPET biodegradationInvestigaciónPlastic pollution is a growing concern, especially poly(ethylene terephthalate) (PET), one of the most produced plastic polymers. Although several microorganisms capable of degrading PET have been identified, little is known about those present in wastewater treatment plants (WWTPs). This study explores their ability to degrade PET and the enzymes involved. Activated sludge from two facilities—one urban WWTP and one industrial WWTP—was cultivated with PET of different crystallinities. The inoculum source primarily determined differences in microbial community composition. Metagenomics revealed more than 300 genes homologous to PET-degrading enzymes in all biofilms; however, metaproteomics confirmed expression of only a few of these enzymes in industrial WWTP-derived biofilms. This inoculum demonstrated the ability to degrade PET breakdown products within 24 h. In addition, FTIR analysis revealed initial signs of surface alteration. In conclusion, this study reveals the presence of microorganisms in industrial wastewater treatment sludge that possess the genetic potential to degrade PET.ElsevierUniversidade de Santiago de Compostela. Departamento de Enxeñaría QuímicaUniversidade de Santiago de Compostela. Departamento de Microbioloxía e ParasitoloxíaUniversidade de Santiago de Compostela. Departamento de Química FísicaUniversidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)20262026-03-0120262026-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/46844reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© 2026 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license ( http://creativecommons.org/licenses/by-nc/4.0/ ).http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:dnet:minerva_____::d83d797afd10eea376d7e53001453a832026-06-15T12:47:27Z
dc.title.none.fl_str_mv Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
title Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
spellingShingle Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
Vijande, Carlota
Biofilm
Metaproteomics
Metagenomics
PET biodegradation
Investigación
title_short Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
title_full Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
title_fullStr Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
title_full_unstemmed Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
title_sort Multi-omics reveals wastewater sludge bacteria with genomic potential to degrade poly(ethylene terephthalate)
dc.creator.none.fl_str_mv Vijande, Carlota
Balboa Méndez, Sabela
Lazzari, Massimo
Lema Rodicio, Juan Manuel
Pabst, Martin
author Vijande, Carlota
author_facet Vijande, Carlota
Balboa Méndez, Sabela
Lazzari, Massimo
Lema Rodicio, Juan Manuel
Pabst, Martin
author_role author
author2 Balboa Méndez, Sabela
Lazzari, Massimo
Lema Rodicio, Juan Manuel
Pabst, Martin
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Enxeñaría Química
Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
Universidade de Santiago de Compostela. Departamento de Química Física
Universidade de Santiago de Compostela. Centro Interdisciplinar de Investigación en Tecnoloxías Ambientais (CRETUS)
Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS)
dc.subject.none.fl_str_mv Biofilm
Metaproteomics
Metagenomics
PET biodegradation
Investigación
topic Biofilm
Metaproteomics
Metagenomics
PET biodegradation
Investigación
description Plastic pollution is a growing concern, especially poly(ethylene terephthalate) (PET), one of the most produced plastic polymers. Although several microorganisms capable of degrading PET have been identified, little is known about those present in wastewater treatment plants (WWTPs). This study explores their ability to degrade PET and the enzymes involved. Activated sludge from two facilities—one urban WWTP and one industrial WWTP—was cultivated with PET of different crystallinities. The inoculum source primarily determined differences in microbial community composition. Metagenomics revealed more than 300 genes homologous to PET-degrading enzymes in all biofilms; however, metaproteomics confirmed expression of only a few of these enzymes in industrial WWTP-derived biofilms. This inoculum demonstrated the ability to degrade PET breakdown products within 24 h. In addition, FTIR analysis revealed initial signs of surface alteration. In conclusion, this study reveals the presence of microorganisms in industrial wastewater treatment sludge that possess the genetic potential to degrade PET.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026-03-01
2026
2026-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/46844
url https://hdl.handle.net/10347/46844
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411487112495104
score 15.812429

Similares en LA Referencia