Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue

8 Pág.

Detalles Bibliográficos
Autores: Santiago-Moreno, Julián, Peris-Frau, Patricia, Toledano-Díaz, Adolfo, Castaño-Herrero, Cristina, Velázquez, Rosario, Alba, Esther, Gómez-Guillamón, Félix, Camacho, Leonor, Prieto, Paloma, Martínez-Madrid, Belén
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/373462
Acceso en línea:http://hdl.handle.net/10261/373462
Access Level:acceso abierto
Palabra clave:PGAM2
Ibex
Lactoferrin
Mouflon
Sperm freezing
Testes vitrification
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spelling Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissueSantiago-Moreno, JuliánPeris-Frau, PatriciaToledano-Díaz, AdolfoCastaño-Herrero, CristinaVelázquez, RosarioAlba, EstherGómez-Guillamón, FélixCamacho, LeonorPrieto, PalomaMartínez-Madrid, BelénPGAM2IbexLactoferrinMouflonSperm freezingTestes vitrification8 Pág.Spermatozoa collected from the cauda epididymis of wild ruminants are more cryoresistant than are ejaculated spermatozoa. This work examines the effects of lactoferrin (LF) and phosphoglycerate mutase 2 (PGAM2), which are abundant in the epididymal sperm of wild ruminants, as additives in Iberian ibex and mouflon sperm extenders. In addition, LF was added to a vitrification medium to determine whether it also provided protection during the cryopreservation of testicular tissue. Epididymal sperm samples and testes were recovered post-mortem from ibexes (n=13) and mouflons (n=8) during the breeding season. Ejaculates were collected from both species (n=10 each) using the transrectal ultrasound-guided massage technique. Four aliquots were taken from each sample, diluted in the appropriate freezing extender for each species, and subjected to the following treatments: control, LF (100 μg/ml), PGAM2 (25 μg/ml), and LF+PGAM2. Testicular tissue was cut into small pieces and cryopreserved by needle-immersed vitrification in a medium with or without LF. In vivo fertilization capacity was assessed using frozen-thawed ejaculated sperm from the ibexes. Supplementation of extenders with LF or PGAM2, and their combination, had no beneficial or harmful effect on any sperm variable after freezing-thawing. Artificial insemination of ibexes showed that the fertility rate in controls was 62.5%, but this fell to 20% in females inseminated with sperm treated with LF (p = 0.06), suggesting a putative negative effect of LF on fertility. The viability of elongated spermatids and spermatozoa from mouflon testes was greater (p < 0.05) in samples that were vitrified-warmed with LF. Thus, the hypothesis that supplementing ejaculated sperm with LF and/or PGAM2 improves sperm quality after freeze-thawing was not upheld. However, LF would seem an appropriate additive when vitrifying mouflon testicular tissue.This study was funded via project PID2020-113288RBI00/AEI/10.13039/501100011033.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Santiago Moreno, Julián [0000-0001-5551-8120]Peris-Frau, Patricia [0000-0001-6266-0635]Toledano-Díaz, A. [0000-0001-6679-485X]Castaño, Cristina [0000-0003-1134-1436]Velázquez, Rosario [0009-0009-2115-4715]Alba, Esther [0009-0009-2603-777X]Gómez-Guillamón, Félix [0000-0001-9757-9589]Martínez-Madrid, Belén [0000-0001-6852-4597]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/373462reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00Departamento de Reproducción Animal​ https://doi.org/10.1016/j.cryobiol.2024.105182Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3734622026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
title Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
spellingShingle Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
Santiago-Moreno, Julián
PGAM2
Ibex
Lactoferrin
Mouflon
Sperm freezing
Testes vitrification
title_short Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
title_full Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
title_fullStr Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
title_full_unstemmed Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
title_sort Effect of exogenous lactoferrin and phosphoglycerate mutase 2 on the cryopreservation of wild ruminant epididymal/ejaculated sperm and testicular tissue
dc.creator.none.fl_str_mv Santiago-Moreno, Julián
Peris-Frau, Patricia
Toledano-Díaz, Adolfo
Castaño-Herrero, Cristina
Velázquez, Rosario
Alba, Esther
Gómez-Guillamón, Félix
Camacho, Leonor
Prieto, Paloma
Martínez-Madrid, Belén
author Santiago-Moreno, Julián
author_facet Santiago-Moreno, Julián
Peris-Frau, Patricia
Toledano-Díaz, Adolfo
Castaño-Herrero, Cristina
Velázquez, Rosario
Alba, Esther
Gómez-Guillamón, Félix
Camacho, Leonor
Prieto, Paloma
Martínez-Madrid, Belén
author_role author
author2 Peris-Frau, Patricia
Toledano-Díaz, Adolfo
Castaño-Herrero, Cristina
Velázquez, Rosario
Alba, Esther
Gómez-Guillamón, Félix
Camacho, Leonor
Prieto, Paloma
Martínez-Madrid, Belén
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Santiago Moreno, Julián [0000-0001-5551-8120]
Peris-Frau, Patricia [0000-0001-6266-0635]
Toledano-Díaz, A. [0000-0001-6679-485X]
Castaño, Cristina [0000-0003-1134-1436]
Velázquez, Rosario [0009-0009-2115-4715]
Alba, Esther [0009-0009-2603-777X]
Gómez-Guillamón, Félix [0000-0001-9757-9589]
Martínez-Madrid, Belén [0000-0001-6852-4597]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PGAM2
Ibex
Lactoferrin
Mouflon
Sperm freezing
Testes vitrification
topic PGAM2
Ibex
Lactoferrin
Mouflon
Sperm freezing
Testes vitrification
description 8 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/373462
url http://hdl.handle.net/10261/373462
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00
Departamento de Reproducción Animal​ 
https://doi.org/10.1016/j.cryobiol.2024.105182
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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