Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision

25 págs, 5 figuras, Supplementary material: 8 figs, 2 tablas

Detalles Bibliográficos
Autores: Zamora-Caballero, Sara, Chmielowska, Cora, Quiles-Puchalt, Nuria, Brady, Aisling, Gallego del Sol, Francisca, Mancheño-Bonillo, Javier, Felipe-Ruiz, Alonso, Meijer, Wilfried J. J., Penadés, José R., Marina, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/358426
Acceso en línea:http://hdl.handle.net/10261/358426
Access Level:acceso abierto
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spelling Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decisionZamora-Caballero, SaraChmielowska, CoraQuiles-Puchalt, NuriaBrady, AislingGallego del Sol, FranciscaMancheño-Bonillo, JavierFelipe-Ruiz, AlonsoMeijer, Wilfried J. J.Penadés, José R.Marina, Alberto25 págs, 5 figuras, Supplementary material: 8 figs, 2 tablasPhages can use a small-molecule communication arbitrium system to coordinate lysis-lysogeny decisions, but the underlying mechanism remains unknown. Here we determined that the arbitrium system in Bacillus subtilis phage phi3T modulates the bacterial toxin-antitoxin system MazE-MazF to regulate the phage life cycle. We show that phi3T expresses AimX and YosL, which bind to and inactivate MazF. AimX also inhibits the function of phi3T_93, a protein that promotes lysogeny by binding to MazE and releasing MazF. Overall, these mutually exclusive interactions promote the lytic cycle of the phage. After several rounds of infection, the phage-encoded AimP peptide accumulates intracellularly and inactivates the phage antiterminator AimR, a process that eliminates aimX expression from the aimP promoter. Therefore, when AimP increases, MazF activity promotes reversion back to lysogeny, since AimX is absent. Altogether, our study reveals the evolutionary strategy used by arbitrium to control lysis-lysogeny by domesticating and fine-tuning a phage-defence mechanism.This work was supported by grants PID2019-108541GB-I00 and PID2022-137201NB-I00 from the Spanish Government (Ministerio de Ciencia e Innovación), PROMETEO/2020/012 by the Valencian Government and the European Commission NextGenerationEU fund (EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global) to A.M., and grants MR/M003876/1, MR/V000772/1 and MR/ S00940X/1 from the Medical Research Council (UK), BB/N002873/1, BB/V002376/1 and BB/S003835/1 from the Biotechnology and Biological Sciences Research Council (BBSRC, UK), ERC-ADG-2014 Proposal no. 670932 Dut-signal (from EU), and Wellcome Trust 201531/Z/16/Z to J.R.P. A.F.-R. received an FPU predoctoral fellowship from the Spanish Ministry of Universities, reference FPU19/00433Peer reviewedNature Publishing GroupMinisterio de Ciencia e Innovación (España)Generalitat ValencianaEuropean CommissionCSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global)Medical Research Council (UK)Ministerio de Universidades (España)Biotechnology and Biological Sciences Research Council (UK)Marina, Alberto [0000-0002-1334-5273]Zamora-Caballero, Sara [0000-0003-4717-8845]Gallego del Sol, Francisca [0000-0001-9862-4505]Mancheño-Bonillo, Javier [0000-0001-5664-8752]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/358426reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108541GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137201NB-I00info:eu-repo/grantAgreement/EC/H2020/2094The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1038/s41564-023-01550-4https://doi.org/10.1038/s41564-023-01550-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3584262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
title Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
spellingShingle Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
Zamora-Caballero, Sara
title_short Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
title_full Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
title_fullStr Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
title_full_unstemmed Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
title_sort Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision
dc.creator.none.fl_str_mv Zamora-Caballero, Sara
Chmielowska, Cora
Quiles-Puchalt, Nuria
Brady, Aisling
Gallego del Sol, Francisca
Mancheño-Bonillo, Javier
Felipe-Ruiz, Alonso
Meijer, Wilfried J. J.
Penadés, José R.
Marina, Alberto
author Zamora-Caballero, Sara
author_facet Zamora-Caballero, Sara
Chmielowska, Cora
Quiles-Puchalt, Nuria
Brady, Aisling
Gallego del Sol, Francisca
Mancheño-Bonillo, Javier
Felipe-Ruiz, Alonso
Meijer, Wilfried J. J.
Penadés, José R.
Marina, Alberto
author_role author
author2 Chmielowska, Cora
Quiles-Puchalt, Nuria
Brady, Aisling
Gallego del Sol, Francisca
Mancheño-Bonillo, Javier
Felipe-Ruiz, Alonso
Meijer, Wilfried J. J.
Penadés, José R.
Marina, Alberto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
European Commission
CSIC - Plataforma Temática Interdisciplinar del CSIC Salud Global (PTI Salud Global)
Medical Research Council (UK)
Ministerio de Universidades (España)
Biotechnology and Biological Sciences Research Council (UK)
Marina, Alberto [0000-0002-1334-5273]
Zamora-Caballero, Sara [0000-0003-4717-8845]
Gallego del Sol, Francisca [0000-0001-9862-4505]
Mancheño-Bonillo, Javier [0000-0001-5664-8752]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 25 págs, 5 figuras, Supplementary material: 8 figs, 2 tablas
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/358426
url http://hdl.handle.net/10261/358426
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108541GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137201NB-I00
info:eu-repo/grantAgreement/EC/H2020/2094
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1038/s41564-023-01550-4
https://doi.org/10.1038/s41564-023-01550-4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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