Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays

Quantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles...

Descripción completa

Detalles Bibliográficos
Autores: García-Manrique, Pablo, Serrano-Pertierra, Esther, Lozano-Andrés, Estefanía, López-Martín, Soraya, Matos, María, Gutiérrez, Gemma, Yáñez-Mó, María, Blanco-López, María Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/238925
Acceso en línea:http://hdl.handle.net/10261/238925
Access Level:acceso abierto
Palabra clave:Artificial extracellular vesicles (EVs)
Tetraspanins
Analytical standards
Immunoassays development
Biomimetic materials
id ES_7af7ec04320c4b5a5e0fa487cecbef9a
oai_identifier_str oai:digital.csic.es:10261/238925
network_acronym_str ES
network_name_str España
repository_id_str
spelling Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassaysGarcía-Manrique, PabloSerrano-Pertierra, EstherLozano-Andrés, EstefaníaLópez-Martín, SorayaMatos, MaríaGutiérrez, GemmaYáñez-Mó, MaríaBlanco-López, María CarmenArtificial extracellular vesicles (EVs)TetraspaninsAnalytical standardsImmunoassays developmentBiomimetic materialsQuantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles with morphological and functional properties close to natural exosomes. This work presents the design, development and functional characteristics of fully artificial exosomes based on tetraspanin extracellular loops-coated niosomes, produced by bio-nanotechnology methods based on supra-molecular chemistry and recombinant protein technology. Mono-and double-functionalized particles with CD9/CD63 tetraspanins have been developed and characterized from a morphological and functional point of view. Produced bio-particles showed close similarities with natural entities in terms of physical properties. Their utility for bioanalysis is demonstrated by their detection and molecular-type discrimination by enzyme-linked immunosorbent assays (ELISAs), one of the most frequent bio-analytical method found in routine and research labs. The basic material based on streptavidin-coated niosomes allows the surface functionalization with any biotinylated protein or peptide, introducing versatility. Although promising results have been reported, further optimizations and deeper characterization will help this innovative biomaterial become a robust RM for validation and development of diagnostic tools for exosomes determination.Ministerio de Economía y Competitividad (MINECO, Spain), grant number CTQ2013-47396-R and number MAT2017-84959-C2-1-R, and grants BFU2014-5578R and BIO2017-86500-R to Dr Yáñez-Mó. This study was also financed by the Consejería de Economía y Empleo del Principado de Asturias (Plan de Ciencia, Tecnología e Innovación 2013-2017), under the Grant GRUPIN14-022 and IDI2018/000185. Support from the European Regional Development Fund (ERDF)Ministerio de Economía y Competitividad (España)Principado de AsturiasConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/238925reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/nano10050971Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2389252026-05-22T06:33:51Z
dc.title.none.fl_str_mv Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
title Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
spellingShingle Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
García-Manrique, Pablo
Artificial extracellular vesicles (EVs)
Tetraspanins
Analytical standards
Immunoassays development
Biomimetic materials
title_short Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
title_full Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
title_fullStr Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
title_full_unstemmed Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
title_sort Selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays
dc.creator.none.fl_str_mv García-Manrique, Pablo
Serrano-Pertierra, Esther
Lozano-Andrés, Estefanía
López-Martín, Soraya
Matos, María
Gutiérrez, Gemma
Yáñez-Mó, María
Blanco-López, María Carmen
author García-Manrique, Pablo
author_facet García-Manrique, Pablo
Serrano-Pertierra, Esther
Lozano-Andrés, Estefanía
López-Martín, Soraya
Matos, María
Gutiérrez, Gemma
Yáñez-Mó, María
Blanco-López, María Carmen
author_role author
author2 Serrano-Pertierra, Esther
Lozano-Andrés, Estefanía
López-Martín, Soraya
Matos, María
Gutiérrez, Gemma
Yáñez-Mó, María
Blanco-López, María Carmen
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Principado de Asturias
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Artificial extracellular vesicles (EVs)
Tetraspanins
Analytical standards
Immunoassays development
Biomimetic materials
topic Artificial extracellular vesicles (EVs)
Tetraspanins
Analytical standards
Immunoassays development
Biomimetic materials
description Quantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles with morphological and functional properties close to natural exosomes. This work presents the design, development and functional characteristics of fully artificial exosomes based on tetraspanin extracellular loops-coated niosomes, produced by bio-nanotechnology methods based on supra-molecular chemistry and recombinant protein technology. Mono-and double-functionalized particles with CD9/CD63 tetraspanins have been developed and characterized from a morphological and functional point of view. Produced bio-particles showed close similarities with natural entities in terms of physical properties. Their utility for bioanalysis is demonstrated by their detection and molecular-type discrimination by enzyme-linked immunosorbent assays (ELISAs), one of the most frequent bio-analytical method found in routine and research labs. The basic material based on streptavidin-coated niosomes allows the surface functionalization with any biotinylated protein or peptide, introducing versatility. Although promising results have been reported, further optimizations and deeper characterization will help this innovative biomaterial become a robust RM for validation and development of diagnostic tools for exosomes determination.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/238925
url http://hdl.handle.net/10261/238925
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/nano10050971
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411479329964032
score 15,811543

Similares en LA Referencia