Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi

Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can par...

ver descrição completa

Detalhes bibliográficos
Autores: Sorribes-Dauden, R, Peris Navarro, David, Martínez-Pastor, María Teresa, Puig, Sergi
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/239152
Acesso em linha:http://hdl.handle.net/10261/239152
Access Level:acceso abierto
Palavra-chave:Iron detoxification
Iron regulation
Iron transport
Yeast
Fungi
Plants
Vacuole
Ccc1
VIT1
id ES_7af4a41da09af4ece61ae7a12611fba2
oai_identifier_str oai:digital.csic.es:10261/239152
network_acronym_str ES
network_name_str España
repository_id_str
spelling Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungiSorribes-Dauden, RPeris Navarro, DavidMartínez-Pastor, María TeresaPuig, SergiIron detoxificationIron regulationIron transportYeastFungiPlantsVacuoleCcc1VIT1Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can participate in Fenton reactions and generate reactive oxygen species that damage cells at the level of proteins, lipids and nucleic acids. Organisms have developed different molecular strategies to protect themselves against the harmful effects of high concentrations of iron. In the case of fungi and plants, detoxification mainly occurs by importing cytosolic iron into the vacuole through the Ccc1/VIT1 iron transporter. New sequenced genomes and bioinformatic tools are facilitating the functional characterization, evolution and ecological relevance of metabolic pathways and homeostatic networks across the Tree of Life. Sequence analysis shows that Ccc1/VIT1 homologs are widely distributed among organisms with the exception of animals. The recent elucidation of the crystal structure of a Ccc1/VIT1 plant ortholog has enabled the identification of both conserved and species-specific motifs required for its metal transport mechanism. Moreover, recent studies in the yeast Saccharomyces cerevisiae have also revealed that multiple transcription factors including Yap5 and Msn2/Msn4 contribute to the expression of CCC1 in high-iron conditions. Interestingly, Malaysian S. cerevisiae strains express a partially functional Ccc1 protein that renders them sensitive to iron. Different regulatory mechanisms have been described for non-Saccharomycetaceae Ccc1 homologs. The characterization of Ccc1/VIT1 proteins is of high interest in the development of biofortified crops and the protection against microbial-derived diseases.Work in our laboratory is supported by the Spanish Ministry of Science, Innovation and Universities (MICINN) grant BIO2017-87828-C2-1-P, the Regional Government of Valencia “Generalitat Valenciana” grant PROMETEU/2020/014 and FEDER (Fondo Europeo de Desarrollo Regional) funds to SP, and a predoctoral contract from “Generalitat Valenciana” and FEDER funds to RSD. Computations were performed on Tirant III of the Spanish Supercomputing Network (“Servei d'Informàtica de la Universitat de València”) under the project BCV-2018-2-0002 granted to DP.Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Generalitat ValencianaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/239152reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2017-87828-C2-1-Phttps://doi.org/10.1016/j.csbj.2020.10.044Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2391522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
title Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
spellingShingle Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
Sorribes-Dauden, R
Iron detoxification
Iron regulation
Iron transport
Yeast
Fungi
Plants
Vacuole
Ccc1
VIT1
title_short Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
title_full Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
title_fullStr Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
title_full_unstemmed Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
title_sort Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
dc.creator.none.fl_str_mv Sorribes-Dauden, R
Peris Navarro, David
Martínez-Pastor, María Teresa
Puig, Sergi
author Sorribes-Dauden, R
author_facet Sorribes-Dauden, R
Peris Navarro, David
Martínez-Pastor, María Teresa
Puig, Sergi
author_role author
author2 Peris Navarro, David
Martínez-Pastor, María Teresa
Puig, Sergi
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Generalitat Valenciana
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Iron detoxification
Iron regulation
Iron transport
Yeast
Fungi
Plants
Vacuole
Ccc1
VIT1
topic Iron detoxification
Iron regulation
Iron transport
Yeast
Fungi
Plants
Vacuole
Ccc1
VIT1
description Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can participate in Fenton reactions and generate reactive oxygen species that damage cells at the level of proteins, lipids and nucleic acids. Organisms have developed different molecular strategies to protect themselves against the harmful effects of high concentrations of iron. In the case of fungi and plants, detoxification mainly occurs by importing cytosolic iron into the vacuole through the Ccc1/VIT1 iron transporter. New sequenced genomes and bioinformatic tools are facilitating the functional characterization, evolution and ecological relevance of metabolic pathways and homeostatic networks across the Tree of Life. Sequence analysis shows that Ccc1/VIT1 homologs are widely distributed among organisms with the exception of animals. The recent elucidation of the crystal structure of a Ccc1/VIT1 plant ortholog has enabled the identification of both conserved and species-specific motifs required for its metal transport mechanism. Moreover, recent studies in the yeast Saccharomyces cerevisiae have also revealed that multiple transcription factors including Yap5 and Msn2/Msn4 contribute to the expression of CCC1 in high-iron conditions. Interestingly, Malaysian S. cerevisiae strains express a partially functional Ccc1 protein that renders them sensitive to iron. Different regulatory mechanisms have been described for non-Saccharomycetaceae Ccc1 homologs. The characterization of Ccc1/VIT1 proteins is of high interest in the development of biofortified crops and the protection against microbial-derived diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/239152
url http://hdl.handle.net/10261/239152
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BIO2017-87828-C2-1-P
https://doi.org/10.1016/j.csbj.2020.10.044
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411478341156864
score 15.811543

Registos relacionados