Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.

Han, Bing; Molins López-Rodó, Laureano; He, Yangyi; Viñolas Segarra, Núria; Sánchez Lorente, David; Boada, Marc; Guirao Montes, Àngela; Díaz Sánchez, Tania; Martinez, Daniel; Ramirez, José; Moisés, Jorge; Acosta Plasencia, Melissa; Monzó Planella, Mariano; Marrades Sicart, Ramon Ma.; Navarro Ponz, Alfons

Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.

In resected non-small cell lung cancer (NSCLC), post-surgical recurrence occurs in around 40% of patients, highlighting the necessity to identify relapse biomarkers. An analysis of the extracellular vesicle (EV) cargo from a pulmonary tumor-draining vein (TDV) can grant biomarker identification. We...

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Autores:	Han, Bing, Molins López-Rodó, Laureano, He, Yangyi, Viñolas Segarra, Núria, Sánchez Lorente, David, Boada, Marc, Guirao Montes, Àngela, Díaz Sánchez, Tania, Martinez, Daniel, Ramirez, José, Moisés, Jorge, Acosta Plasencia, Melissa, Monzó Planella, Mariano, Marrades Sicart, Ramon Ma., Navarro Ponz, Alfons
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2022
País:	España
Institución:	Universidad de Barcelona
Repositorio:	Dipòsit Digital de la UB
OAI Identifier:	oai:diposit.ub.edu:2445/189870
Acceso en línea:	https://hdl.handle.net/2445/189870
Access Level:	acceso abierto
Palabra clave:	Marcadors tumorals Càncer de pulmó Micro RNAs Tumor markers Lung cancer MicroRNAs

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spelling	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.Han, BingMolins López-Rodó, LaureanoHe, YangyiViñolas Segarra, NúriaSánchez Lorente, DavidBoada, MarcGuirao Montes, ÀngelaDíaz Sánchez, TaniaMartinez, DanielRamirez, JoséMoisés, JorgeAcosta Plasencia, MelissaMonzó Planella, MarianoMarrades Sicart, Ramon Ma.Navarro Ponz, AlfonsMarcadors tumoralsCàncer de pulmóMicro RNAsTumor markersLung cancerMicroRNAsIn resected non-small cell lung cancer (NSCLC), post-surgical recurrence occurs in around 40% of patients, highlighting the necessity to identify relapse biomarkers. An analysis of the extracellular vesicle (EV) cargo from a pulmonary tumor-draining vein (TDV) can grant biomarker identification. We studied the pulmonary TDV EV-miRNAome to identify relapse biomarkers in a two-phase study (screening and validation). In the screening phase, a 17-miRNA relapse signature was identified in 18 selected patients by small RNAseq. The most expressed miRNA from the signature (EV-miR-203a-3p) was chosen for further validation. Pulmonary TDV EV-miR-203a-3p was studied by qRT-PCR in a validation cohort of 70 patients, where it was found to be upregulated in relapsed patients (p = 0.0194) and in patients with cancer spread to nearby lymph nodes (N+ patients) (p = 0.0396). The ROC curve analysis showed that TDV EV-miR-203a-3p was able to predict relapses with a sensitivity of 88% (AUC: 0.67; p = 0.022). Moreover, patients with high TDV EV-miR-203a-3p had a shorter time to relapse than patients with low levels (43.6 vs. 97.6 months; p = 0.00703). The multivariate analysis showed that EV-miR-203a-3p was an independent, predictive and prognostic post-surgical relapse biomarker. In conclusion, pulmonary TDV EV-miR-203a-3p is a promising new relapse biomarker for resected NSCLC patients.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/189870Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms23137138International Journal of Molecular Sciences, 2022, vol. 23, num. 13, p. 7138https://doi.org/10.3390/ijms23137138cc-by (c) Han, Bing et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1898702026-05-27T06:46:51Z
dc.title.none.fl_str_mv	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
title	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
spellingShingle	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer. Han, Bing Marcadors tumorals Càncer de pulmó Micro RNAs Tumor markers Lung cancer MicroRNAs
title_short	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
title_full	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
title_fullStr	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
title_full_unstemmed	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
title_sort	Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.
dc.creator.none.fl_str_mv	Han, Bing Molins López-Rodó, Laureano He, Yangyi Viñolas Segarra, Núria Sánchez Lorente, David Boada, Marc Guirao Montes, Àngela Díaz Sánchez, Tania Martinez, Daniel Ramirez, José Moisés, Jorge Acosta Plasencia, Melissa Monzó Planella, Mariano Marrades Sicart, Ramon Ma. Navarro Ponz, Alfons
author	Han, Bing
author_facet	Han, Bing Molins López-Rodó, Laureano He, Yangyi Viñolas Segarra, Núria Sánchez Lorente, David Boada, Marc Guirao Montes, Àngela Díaz Sánchez, Tania Martinez, Daniel Ramirez, José Moisés, Jorge Acosta Plasencia, Melissa Monzó Planella, Mariano Marrades Sicart, Ramon Ma. Navarro Ponz, Alfons
author_role	author
author2	Molins López-Rodó, Laureano He, Yangyi Viñolas Segarra, Núria Sánchez Lorente, David Boada, Marc Guirao Montes, Àngela Díaz Sánchez, Tania Martinez, Daniel Ramirez, José Moisés, Jorge Acosta Plasencia, Melissa Monzó Planella, Mariano Marrades Sicart, Ramon Ma. Navarro Ponz, Alfons
author2_role	author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Marcadors tumorals Càncer de pulmó Micro RNAs Tumor markers Lung cancer MicroRNAs
topic	Marcadors tumorals Càncer de pulmó Micro RNAs Tumor markers Lung cancer MicroRNAs
description	In resected non-small cell lung cancer (NSCLC), post-surgical recurrence occurs in around 40% of patients, highlighting the necessity to identify relapse biomarkers. An analysis of the extracellular vesicle (EV) cargo from a pulmonary tumor-draining vein (TDV) can grant biomarker identification. We studied the pulmonary TDV EV-miRNAome to identify relapse biomarkers in a two-phase study (screening and validation). In the screening phase, a 17-miRNA relapse signature was identified in 18 selected patients by small RNAseq. The most expressed miRNA from the signature (EV-miR-203a-3p) was chosen for further validation. Pulmonary TDV EV-miR-203a-3p was studied by qRT-PCR in a validation cohort of 70 patients, where it was found to be upregulated in relapsed patients (p = 0.0194) and in patients with cancer spread to nearby lymph nodes (N+ patients) (p = 0.0396). The ROC curve analysis showed that TDV EV-miR-203a-3p was able to predict relapses with a sensitivity of 88% (AUC: 0.67; p = 0.022). Moreover, patients with high TDV EV-miR-203a-3p had a shorter time to relapse than patients with low levels (43.6 vs. 97.6 months; p = 0.00703). The multivariate analysis showed that EV-miR-203a-3p was an independent, predictive and prognostic post-surgical relapse biomarker. In conclusion, pulmonary TDV EV-miR-203a-3p is a promising new relapse biomarker for resected NSCLC patients.
publishDate	2022
dc.date.none.fl_str_mv	2022
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/189870
url	https://hdl.handle.net/2445/189870
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.3390/ijms23137138 International Journal of Molecular Sciences, 2022, vol. 23, num. 13, p. 7138 https://doi.org/10.3390/ijms23137138
dc.rights.none.fl_str_mv	cc-by (c) Han, Bing et al., 2022 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Han, Bing et al., 2022 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona
instname_str	Universidad de Barcelona
reponame_str	Dipòsit Digital de la UB
collection	Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Characterization of the MicroRNA Cargo of Extracellular Vesicles Isolated from a Pulmonary Tumor-Draining Vein Identifies miR-203a-3p as a Relapse Biomarker for Resected Non-Small Cell Lung Cancer.

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