Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain.
Background: Surveillance tools to estimate viral transmission dynamics in young populations are essential to guide recommendations for school opening and management during viral epidemics. Ideally, sensitive techniques are required to detect low viral load exposures among asymptomatic children. We a...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/184331 |
| Acceso en línea: | https://hdl.handle.net/2445/184331 |
| Access Level: | acceso abierto |
| Palabra clave: | SARS-CoV-2 Infants Entorn escolar Saliva Antígens Children School environment Antigens |
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Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain.Dobaño, Carlota, 1969-Alonso, SelenaFernández de Sevilla Estrach, MarionaVidal, MartaJiménez, AlfonsPons Tomàs, GemmaJairoce, Chenjerai Tobias SixpenceMelé Casas, MariaRubio, RocíoHernández García, MaríaRuiz Olalla, GemmaGirona Alarcón, MònicaBarrios, DianaSantano, RebecaMitchell, Robert AndrewPuyol, LauraMayer, LeonieChi, JordiRodrigo Melero, NataliaCarolis, CarloGarcia Miquel, AleixBonet Carné, ElisendaClaverol, JoanaCubells, MartaFortuny Guasch, ClaudiaFumadó, VictoriaJou, CristinaMuñoz Almagro, CarmenIzquierdo Lázaro, LuisBassat Orellana, QuiqueGratacós Solsona, EduardAguilar, RuthGarcía García, Juan JoséMoncunill Piñas, GemmaJordán García, IolandaSARS-CoV-2InfantsEntorn escolarSalivaAntígensSARS-CoV-2ChildrenSchool environmentSalivaAntigensBackground: Surveillance tools to estimate viral transmission dynamics in young populations are essential to guide recommendations for school opening and management during viral epidemics. Ideally, sensitive techniques are required to detect low viral load exposures among asymptomatic children. We aimed to estimate SARS-CoV-2 infection rates in children and adult populations in a school-like environment during the initial COVID-19 pandemic waves using an antibody-based field-deployable and non-invasive approach. Methods: Saliva antibody conversion defined as ≥ 4-fold increase in IgM, IgA, and/or IgG levels to five SARS-CoV-2 antigens including spike and nucleocapsid constructs was evaluated in 1509 children and 396 adults by high-throughput Luminex assays in samples collected weekly in 22 summer schools and 2 pre-schools in 27 venues in Barcelona, Spain, from June 29th to July 31st, 2020. Results: Saliva antibody conversion between two visits over a 5-week period was 3.22% (49/1518) or 2.36% if accounting for potentially cross-reactive antibodies, six times higher than the cumulative infection rate (0.53%) assessed by weekly saliva RT-PCR screening. IgG conversion was higher in adults (2.94%, 11/374) than children (1.31%, 15/1144) (p=0.035), IgG and IgA levels moderately increased with age, and antibodies were higher in females. Most antibody converters increased both IgG and IgA antibodies but some augmented either IgG or IgA, with a faster decay over time for IgA than IgG. Nucleocapsid rather than spike was the main antigen target. Anti-spike antibodies were significantly higher in individuals not reporting symptoms than symptomatic individuals, suggesting a protective role against COVID-19. Conclusion: Saliva antibody profiling including three isotypes and multiplexing antigens is a useful and user-friendlier tool for screening pediatric populations to detect low viral load exposures among children, particularly while they are not vaccinated and vulnerable to highly contagious variants, and to recommend public health policies during pandemics.BioMed Central2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/184331Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/s12916-021-02184-1BMC Medicine, 2021, vol. 19, num. 1, p. 309https://doi.org/10.1186/s12916-021-02184-1cc-by (c) Dobaño, Carlota, 1969- et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1843312026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| title |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| spellingShingle |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. Dobaño, Carlota, 1969- SARS-CoV-2 Infants Entorn escolar Saliva Antígens SARS-CoV-2 Children School environment Saliva Antigens |
| title_short |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| title_full |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| title_fullStr |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| title_full_unstemmed |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| title_sort |
Antibody conversion rates to SARS-CoV-2 in saliva from children attending summer schools in Barcelona, Spain. |
| dc.creator.none.fl_str_mv |
Dobaño, Carlota, 1969- Alonso, Selena Fernández de Sevilla Estrach, Mariona Vidal, Marta Jiménez, Alfons Pons Tomàs, Gemma Jairoce, Chenjerai Tobias Sixpence Melé Casas, Maria Rubio, Rocío Hernández García, María Ruiz Olalla, Gemma Girona Alarcón, Mònica Barrios, Diana Santano, Rebeca Mitchell, Robert Andrew Puyol, Laura Mayer, Leonie Chi, Jordi Rodrigo Melero, Natalia Carolis, Carlo Garcia Miquel, Aleix Bonet Carné, Elisenda Claverol, Joana Cubells, Marta Fortuny Guasch, Claudia Fumadó, Victoria Jou, Cristina Muñoz Almagro, Carmen Izquierdo Lázaro, Luis Bassat Orellana, Quique Gratacós Solsona, Eduard Aguilar, Ruth García García, Juan José Moncunill Piñas, Gemma Jordán García, Iolanda |
| author |
Dobaño, Carlota, 1969- |
| author_facet |
Dobaño, Carlota, 1969- Alonso, Selena Fernández de Sevilla Estrach, Mariona Vidal, Marta Jiménez, Alfons Pons Tomàs, Gemma Jairoce, Chenjerai Tobias Sixpence Melé Casas, Maria Rubio, Rocío Hernández García, María Ruiz Olalla, Gemma Girona Alarcón, Mònica Barrios, Diana Santano, Rebeca Mitchell, Robert Andrew Puyol, Laura Mayer, Leonie Chi, Jordi Rodrigo Melero, Natalia Carolis, Carlo Garcia Miquel, Aleix Bonet Carné, Elisenda Claverol, Joana Cubells, Marta Fortuny Guasch, Claudia Fumadó, Victoria Jou, Cristina Muñoz Almagro, Carmen Izquierdo Lázaro, Luis Bassat Orellana, Quique Gratacós Solsona, Eduard Aguilar, Ruth García García, Juan José Moncunill Piñas, Gemma Jordán García, Iolanda |
| author_role |
author |
| author2 |
Alonso, Selena Fernández de Sevilla Estrach, Mariona Vidal, Marta Jiménez, Alfons Pons Tomàs, Gemma Jairoce, Chenjerai Tobias Sixpence Melé Casas, Maria Rubio, Rocío Hernández García, María Ruiz Olalla, Gemma Girona Alarcón, Mònica Barrios, Diana Santano, Rebeca Mitchell, Robert Andrew Puyol, Laura Mayer, Leonie Chi, Jordi Rodrigo Melero, Natalia Carolis, Carlo Garcia Miquel, Aleix Bonet Carné, Elisenda Claverol, Joana Cubells, Marta Fortuny Guasch, Claudia Fumadó, Victoria Jou, Cristina Muñoz Almagro, Carmen Izquierdo Lázaro, Luis Bassat Orellana, Quique Gratacós Solsona, Eduard Aguilar, Ruth García García, Juan José Moncunill Piñas, Gemma Jordán García, Iolanda |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
SARS-CoV-2 Infants Entorn escolar Saliva Antígens SARS-CoV-2 Children School environment Saliva Antigens |
| topic |
SARS-CoV-2 Infants Entorn escolar Saliva Antígens SARS-CoV-2 Children School environment Saliva Antigens |
| description |
Background: Surveillance tools to estimate viral transmission dynamics in young populations are essential to guide recommendations for school opening and management during viral epidemics. Ideally, sensitive techniques are required to detect low viral load exposures among asymptomatic children. We aimed to estimate SARS-CoV-2 infection rates in children and adult populations in a school-like environment during the initial COVID-19 pandemic waves using an antibody-based field-deployable and non-invasive approach. Methods: Saliva antibody conversion defined as ≥ 4-fold increase in IgM, IgA, and/or IgG levels to five SARS-CoV-2 antigens including spike and nucleocapsid constructs was evaluated in 1509 children and 396 adults by high-throughput Luminex assays in samples collected weekly in 22 summer schools and 2 pre-schools in 27 venues in Barcelona, Spain, from June 29th to July 31st, 2020. Results: Saliva antibody conversion between two visits over a 5-week period was 3.22% (49/1518) or 2.36% if accounting for potentially cross-reactive antibodies, six times higher than the cumulative infection rate (0.53%) assessed by weekly saliva RT-PCR screening. IgG conversion was higher in adults (2.94%, 11/374) than children (1.31%, 15/1144) (p=0.035), IgG and IgA levels moderately increased with age, and antibodies were higher in females. Most antibody converters increased both IgG and IgA antibodies but some augmented either IgG or IgA, with a faster decay over time for IgA than IgG. Nucleocapsid rather than spike was the main antigen target. Anti-spike antibodies were significantly higher in individuals not reporting symptoms than symptomatic individuals, suggesting a protective role against COVID-19. Conclusion: Saliva antibody profiling including three isotypes and multiplexing antigens is a useful and user-friendlier tool for screening pediatric populations to detect low viral load exposures among children, particularly while they are not vaccinated and vulnerable to highly contagious variants, and to recommend public health policies during pandemics. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/184331 |
| url |
https://hdl.handle.net/2445/184331 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1186/s12916-021-02184-1 BMC Medicine, 2021, vol. 19, num. 1, p. 309 https://doi.org/10.1186/s12916-021-02184-1 |
| dc.rights.none.fl_str_mv |
cc-by (c) Dobaño, Carlota, 1969- et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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cc-by (c) Dobaño, Carlota, 1969- et al., 2021 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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BioMed Central |
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BioMed Central |
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Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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