Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links

Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regul...

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Authors: González Tarragó, Víctor, Elosegui Artola, Alberto, Bazellières, Elsa, Oria, Roger, Pérez González, Carlos, Roca-Cusachs Soulere, Pere
Format: article
Status:Published version
Publication Date:2017
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/123393
Online Access:https://hdl.handle.net/2445/123393
Access Level:Open access
Keyword:Motilitat cel·lular
Migració cel·lular
Biologia molecular
Cell motility
Cell migration
Molecular biology
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spelling Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin linksGonzález Tarragó, VíctorElosegui Artola, AlbertoBazellières, ElsaOria, RogerPérez González, CarlosRoca-Cusachs Soulere, PereMotilitat cel·lularMigració cel·lularBiologia molecularCell motilityCell migrationMolecular biologyFundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regulating cell migration. However, how this complex affects the α5β1-fn link is unknown. Here we show that the α5β1/ZO-1 complex decreases the resistance to force of α5β1-fn adhesions located at the edge of migrating cell monolayers while also increasing α5β1 recruitment. Consistently with a molecular clutch model of adhesion, this effect of ZO-1 leads to a decrease in the density and intensity of adhesions in cells at the edge of migrating monolayers. Taken together, our results unveil a new mode of integrin regulation through modification of the mechanical properties of integrin-ECM links, which may be harnessed by cells to control adhesion and migration.American Society for Cell Biology2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion6 p.application/pdfhttps://hdl.handle.net/2445/123393Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1091/mbc.E17-01-0006Molecular Biology of the Cell, 2017, vol. 28, num. 14, p. 1847-1852https://doi.org/10.1091/mbc.E17-01-0006info:eu-repo/grantAgreement/EC/FP7/303848cc-by-nc-sa (c) González Tarragó, Víctor et al., 2017http://creativecommons.org/licenses/by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1233932026-05-29T05:05:01Z
dc.title.none.fl_str_mv Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
title Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
spellingShingle Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
González Tarragó, Víctor
Motilitat cel·lular
Migració cel·lular
Biologia molecular
Cell motility
Cell migration
Molecular biology
title_short Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
title_full Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
title_fullStr Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
title_full_unstemmed Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
title_sort Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1-fibronectin links
dc.creator.none.fl_str_mv González Tarragó, Víctor
Elosegui Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez González, Carlos
Roca-Cusachs Soulere, Pere
author González Tarragó, Víctor
author_facet González Tarragó, Víctor
Elosegui Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez González, Carlos
Roca-Cusachs Soulere, Pere
author_role author
author2 Elosegui Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez González, Carlos
Roca-Cusachs Soulere, Pere
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Motilitat cel·lular
Migració cel·lular
Biologia molecular
Cell motility
Cell migration
Molecular biology
topic Motilitat cel·lular
Migració cel·lular
Biologia molecular
Cell motility
Cell migration
Molecular biology
description Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regulating cell migration. However, how this complex affects the α5β1-fn link is unknown. Here we show that the α5β1/ZO-1 complex decreases the resistance to force of α5β1-fn adhesions located at the edge of migrating cell monolayers while also increasing α5β1 recruitment. Consistently with a molecular clutch model of adhesion, this effect of ZO-1 leads to a decrease in the density and intensity of adhesions in cells at the edge of migrating monolayers. Taken together, our results unveil a new mode of integrin regulation through modification of the mechanical properties of integrin-ECM links, which may be harnessed by cells to control adhesion and migration.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/123393
url https://hdl.handle.net/2445/123393
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1091/mbc.E17-01-0006
Molecular Biology of the Cell, 2017, vol. 28, num. 14, p. 1847-1852
https://doi.org/10.1091/mbc.E17-01-0006
info:eu-repo/grantAgreement/EC/FP7/303848
dc.rights.none.fl_str_mv cc-by-nc-sa (c) González Tarragó, Víctor et al., 2017
http://creativecommons.org/licenses/by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa (c) González Tarragó, Víctor et al., 2017
http://creativecommons.org/licenses/by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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