Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria

Antimicrobial Photodynamic Therapy (aPDT) is an emerging strategy against resistant pathogenic bacteria, a serious global health threat. We describe herein the efficient preparation of photosensitized cellulose nanocrystals (CNC) using trialkoxysilane linkers for covalent incorporation of anionic (R...

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Autores: Prieto Montero, Ruth, Tejón Ochandorena, Maite, Albaya, Andrea, Arbeloa López, María Teresa, Chiara, José Luis, López Fanarraga, Mónica, Martínez Martínez, Virginia
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/74502
Acceso en línea:http://hdl.handle.net/10810/74502
Access Level:acceso abierto
Palabra clave:cellulose nanocrystals
photosensitizers
Rose Bengal
toluidine blue O
gluconamide antimicrobial photodynamic therapy
singlet oxygen
gram-negative bacteria
Escherichia coli
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spelling Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteriaPrieto Montero, RuthTejón Ochandorena, MaiteAlbaya, AndreaArbeloa López, María TeresaChiara, José LuisLópez Fanarraga, MónicaMartínez Martínez, Virginiacellulose nanocrystalsphotosensitizersRose Bengaltoluidine blue Ogluconamide antimicrobial photodynamic therapysinglet oxygengram-negative bacteriaEscherichia coliAntimicrobial Photodynamic Therapy (aPDT) is an emerging strategy against resistant pathogenic bacteria, a serious global health threat. We describe herein the efficient preparation of photosensitized cellulose nanocrystals (CNC) using trialkoxysilane linkers for covalent incorporation of anionic (Rose Bengal: RB) and cationic (Toluidine blue O: TBO) photosensitizers (PSs), along with a N-alkyl-D-gluconamide ligand to specifically target Escherichia coli, as model nanosystems for aPDT. The synthesized nanomaterials exhibited high PS loading, high singlet oxygen quantum yield comparable to the solution, and good stability in aqueous media with minimal PS release under physiological conditions. Experimental viability tests in bacteria demonstrated their capability for aPDT, mitigating the inherent cytotoxicity of both PSs under dark conditions while retaining high phototoxicity against E. coli bacteria. The presence of gluconamide further enhanced photoactivity, highlighting the importance of surface functionalization with a specific bacterial ligand for improved efficacy. The CNC-supported RB system exhibited sufficient fluorescence for tracking via fluorescence microscopy, making it suitable for theranostics, integrating bioimaging and aPDT. Overall, photosensitized CNCs hold great promise as nanocarriers for combating topical infections caused by Gram-negative bacteria, addressing the urgent need for novel therapeutic strategies in infectious disease management while also mitigating antimicrobial resistance.This work was funded by MCIN/AEI/10.13039/501100011033 (projects PID2020-114347RB-C32 and PID2020-114347RB-C31, to VMM and JLC, respectively) and European Union NextGenerationEU/PRTR (projects TED2021-129248B-I00 to MLF, and TED2021-132122B-C22 to JLC and VMM), Gobierno Vasco-Eusko Jaurlaritza (project IT1639-22) to VMM, and Spanish Instituto de Salud Carlos III (projects PI22/00030) to MLF. RP-M, MT, and AA. thank MIU and NGEU for the postdoctoral contract (MARSA21/71), Investigo program funding (EU-Next generation), and FPI fellowship (PRE2021-098894), respectively.Elsevier202520252024info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/74502reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1016/j.carbpol.2024.122784info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND licenseoai:addi.ehu.eus:10810/745022026-06-18T09:23:17Z
dc.title.none.fl_str_mv Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
title Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
spellingShingle Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
Prieto Montero, Ruth
cellulose nanocrystals
photosensitizers
Rose Bengal
toluidine blue O
gluconamide antimicrobial photodynamic therapy
singlet oxygen
gram-negative bacteria
Escherichia coli
title_short Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
title_full Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
title_fullStr Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
title_full_unstemmed Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
title_sort Targeted photodynamic therapy: Gluconamide-modified cellulose nanocrystals as efficient photosensitizer delivery platforms against Gram-negative bacteria
dc.creator.none.fl_str_mv Prieto Montero, Ruth
Tejón Ochandorena, Maite
Albaya, Andrea
Arbeloa López, María Teresa
Chiara, José Luis
López Fanarraga, Mónica
Martínez Martínez, Virginia
author Prieto Montero, Ruth
author_facet Prieto Montero, Ruth
Tejón Ochandorena, Maite
Albaya, Andrea
Arbeloa López, María Teresa
Chiara, José Luis
López Fanarraga, Mónica
Martínez Martínez, Virginia
author_role author
author2 Tejón Ochandorena, Maite
Albaya, Andrea
Arbeloa López, María Teresa
Chiara, José Luis
López Fanarraga, Mónica
Martínez Martínez, Virginia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv cellulose nanocrystals
photosensitizers
Rose Bengal
toluidine blue O
gluconamide antimicrobial photodynamic therapy
singlet oxygen
gram-negative bacteria
Escherichia coli
topic cellulose nanocrystals
photosensitizers
Rose Bengal
toluidine blue O
gluconamide antimicrobial photodynamic therapy
singlet oxygen
gram-negative bacteria
Escherichia coli
description Antimicrobial Photodynamic Therapy (aPDT) is an emerging strategy against resistant pathogenic bacteria, a serious global health threat. We describe herein the efficient preparation of photosensitized cellulose nanocrystals (CNC) using trialkoxysilane linkers for covalent incorporation of anionic (Rose Bengal: RB) and cationic (Toluidine blue O: TBO) photosensitizers (PSs), along with a N-alkyl-D-gluconamide ligand to specifically target Escherichia coli, as model nanosystems for aPDT. The synthesized nanomaterials exhibited high PS loading, high singlet oxygen quantum yield comparable to the solution, and good stability in aqueous media with minimal PS release under physiological conditions. Experimental viability tests in bacteria demonstrated their capability for aPDT, mitigating the inherent cytotoxicity of both PSs under dark conditions while retaining high phototoxicity against E. coli bacteria. The presence of gluconamide further enhanced photoactivity, highlighting the importance of surface functionalization with a specific bacterial ligand for improved efficacy. The CNC-supported RB system exhibited sufficient fluorescence for tracking via fluorescence microscopy, making it suitable for theranostics, integrating bioimaging and aPDT. Overall, photosensitized CNCs hold great promise as nanocarriers for combating topical infections caused by Gram-negative bacteria, addressing the urgent need for novel therapeutic strategies in infectious disease management while also mitigating antimicrobial resistance.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/74502
url http://hdl.handle.net/10810/74502
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.carbpol.2024.122784
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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