Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples

[EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification....

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Autores: Pla, Luis, Santiago-Felipe, Sara, Tormo-Mas, María Ángeles, Ruiz-Gaitán, Alba, Peman, Javier, Valentín, Eulogio, Sancenón Galarza, Félix|||0000-0002-5205-7135, Aznar, Elena|||0000-0003-0361-3876, Martínez-Máñez, Ramón|||0000-0001-5873-9674
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/187281
Acceso en línea:https://riunet.upv.es/handle/10251/187281
Access Level:acceso abierto
Palabra clave:Nanoporous anodic alumina
Oligonucleotide
Molecular gates
Candida auris
Biosensor
Rapid diagnosis
QUIMICA ANALITICA
QUIMICA ORGANICA
QUIMICA INORGANICA
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spelling Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samplesPla, LuisSantiago-Felipe, SaraTormo-Mas, María ÁngelesRuiz-Gaitán, AlbaPeman, JavierValentín, EulogioSancenón Galarza, Félix|||0000-0002-5205-7135Aznar, Elena|||0000-0003-0361-3876Martínez-Máñez, Ramón|||0000-0001-5873-9674Nanoporous anodic aluminaOligonucleotideMolecular gatesCandida aurisBiosensorRapid diagnosisQUIMICA ANALITICAQUIMICA ORGANICAQUIMICA INORGANICA[EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. C. auris is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps.The authors want to thank the Spanish Government (project RTI2018-100910-B-C41 (MCIU/AEI/FEDER, UE)), the Generalitat Valenciana (project PROMETEO/2018/024), Universitat Politecnica de Valencia-Instituto de Investigacion Sanitaria La Fe (AURISGATE project) and Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (NANOPATH AND CANDI-EYE projects) for support. L.P thanks MINECO for his predoctoral fellowship. S.S. thanks the Instituto de Salud Carlos III and the European Social Fund for the financial support "Sara Borrell" (CD16/000237).Nature Publishing GroupEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de QuímicaEscuela Técnica Superior de Ingeniería IndustrialInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEuropean Social FundGeneralitat ValencianaInstituto de Salud Carlos IIIAgencia Estatal de InvestigaciónEuropean Regional Development FundUniversitat Politècnica de ValènciaMinisterio de Economía y CompetitividadInstituto de Investigación Sanitaria La FeCentro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y NanomedicinaRepositorio Institucional de la Universitat Politècnica de València Riunet20212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/187281reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-100910-B-C41 MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALAMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CD16%2F00237 CD16Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CD16%2F00237 CD16%2F00237Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2018%2F024 Sistemas avanzados de liberación controladaopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1872812026-06-13T07:49:27Z
dc.title.none.fl_str_mv Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
title Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
spellingShingle Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
Pla, Luis
Nanoporous anodic alumina
Oligonucleotide
Molecular gates
Candida auris
Biosensor
Rapid diagnosis
QUIMICA ANALITICA
QUIMICA ORGANICA
QUIMICA INORGANICA
title_short Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
title_full Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
title_fullStr Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
title_full_unstemmed Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
title_sort Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples
dc.creator.none.fl_str_mv Pla, Luis
Santiago-Felipe, Sara
Tormo-Mas, María Ángeles
Ruiz-Gaitán, Alba
Peman, Javier
Valentín, Eulogio
Sancenón Galarza, Félix|||0000-0002-5205-7135
Aznar, Elena|||0000-0003-0361-3876
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author Pla, Luis
author_facet Pla, Luis
Santiago-Felipe, Sara
Tormo-Mas, María Ángeles
Ruiz-Gaitán, Alba
Peman, Javier
Valentín, Eulogio
Sancenón Galarza, Félix|||0000-0002-5205-7135
Aznar, Elena|||0000-0003-0361-3876
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author_role author
author2 Santiago-Felipe, Sara
Tormo-Mas, María Ángeles
Ruiz-Gaitán, Alba
Peman, Javier
Valentín, Eulogio
Sancenón Galarza, Félix|||0000-0002-5205-7135
Aznar, Elena|||0000-0003-0361-3876
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Química
Escuela Técnica Superior de Ingeniería Industrial
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
European Social Fund
Generalitat Valenciana
Instituto de Salud Carlos III
Agencia Estatal de Investigación
European Regional Development Fund
Universitat Politècnica de València
Ministerio de Economía y Competitividad
Instituto de Investigación Sanitaria La Fe
Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Nanoporous anodic alumina
Oligonucleotide
Molecular gates
Candida auris
Biosensor
Rapid diagnosis
QUIMICA ANALITICA
QUIMICA ORGANICA
QUIMICA INORGANICA
topic Nanoporous anodic alumina
Oligonucleotide
Molecular gates
Candida auris
Biosensor
Rapid diagnosis
QUIMICA ANALITICA
QUIMICA ORGANICA
QUIMICA INORGANICA
description [EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modulation and cargo delivery is controlled by successful DNA recognition. C. auris is detected at a concentration as low as 6 CFU/mL allowing obtaining a diagnostic result in clinical samples in one hour with no prior DNA extraction or amplification steps.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/187281
url https://riunet.upv.es/handle/10251/187281
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-100910-B-C41 MATERIALES POROSOS INTELIGENTES MULTIFUNCIONALES Y DISPOSITIVOS ELECTRONICOS PARA LA LIBERACION DE FARMACOS, DETECCION DE DROGAS Y BIOMARCADORES Y COMUNICACION A NANOESCALA
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CD16%2F00237 CD16
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CD16%2F00237 CD16%2F00237
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2018%2F024 Sistemas avanzados de liberación controlada
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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