Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge

27 Pág.

Detalles Bibliográficos
Autores: Zamora-Ballesteros, Cristina, Pinto, Gloria, Amaral, Joana, Valledor, Luis, Alves, Artur, Díez, Julio Javier, Martín-García, Jorge
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372500
Acceso en línea:http://hdl.handle.net/10261/372500
https://api.elsevier.com/content/abstract/scopus_id/85105712781
Access Level:acceso abierto
Palabra clave:Fusarium circinatum
Pinus pinea
Pinus radiata
Conifer defense
Disease differential susceptibility
Dual RNA-Seq
Host-pathogen interaction
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spelling Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum ChallengeZamora-Ballesteros, CristinaPinto, GloriaAmaral, JoanaValledor, LuisAlves, ArturDíez, Julio JavierMartín-García, JorgeFusarium circinatumPinus pineaPinus radiataConifer defenseDisease differential susceptibilityDual RNA-SeqHost-pathogen interaction27 Pág.Fusarium circinatum causes one of the most important diseases of conifers worldwide, the pine pitch canker (PPC). However, no effective field intervention measures aiming to control or eradicate PPC are available. Due to the variation in host genetic resistance, the development of resistant varieties is postulated as a viable and promising strategy. By using an integrated approach, this study aimed to identify differences in the molecular responses and physiological traits of the highly susceptible Pinus radiata and the highly resistant Pinus pinea to F. circinatum at an early stage of infection. Dual RNA-Seq analysis also allowed to evaluate pathogen behavior when infecting each pine species. No significant changes in the physiological analysis were found upon pathogen infection, although transcriptional reprogramming was observed mainly in the resistant species. The transcriptome profiling of P. pinea revealed an early perception of the pathogen infection together with a strong and coordinated defense activation through the reinforcement and lignification of the cell wall, the antioxidant activity, the induction of PR genes, and the biosynthesis of defense hormones. On the contrary, P. radiata had a weaker response, possibly due to impaired perception of the fungal infection that led to a reduced downstream defense signaling. Fusarium circinatum showed a different transcriptomic profile depending on the pine species being infected. While in P. pinea, the pathogen focused on the degradation of plant cell walls, active uptake of the plant nutrients was showed in P. radiata. These findings present useful knowledge for the development of breeding programs to manage PPC.This research was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade) (POCI-01-FEDER-016785) and by national funds through the Portuguese Foundation for Science and Technology (FCT) within the URGENTpine project (PTDC/AGR-FOR/2768/2014). Thanks are due to FCT/MCTES for financial support to CESAM (UID/50017/2020+UIDB/50017/2020) through national funds. FCT also supported J.A. (SFRH/BD/120967/2016) and JM-G (SFRH/BPD/122928/2016). This study was also made possible through the project PID2019-110459RB-I00 funded by MICINN (Spain) and FEDER (EU) budget as well as the project VA208P20 funded by JCYL (Spain).Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionFundação para a Ciência e a Tecnologia (Portugal)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Zamora-Ballesteros, Cristina [0000-0002-9728-5553]Pinto, Gloria [0000-0001-7735-5131]Amaral, Joana [0000-0002-3572-0070]Valledor, Luis [0000-0002-0636-365X]Alves, Artur [0000-0003-0117-2958]Díez, Julio Javier [0000-0003-0558-8141]Martín-García, Jorge [0000-0002-3057-3710]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/372500https://api.elsevier.com/content/abstract/scopus_id/85105712781reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110459RB-I00Instituto Universitario de Investigación en Gestión Forestal Sostenible (iuFOR)Instituto de Ciencias Forestales (ICIFOR)publisherhttps://doi.org/10.3390/ijms22105231Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3725002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
title Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
spellingShingle Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
Zamora-Ballesteros, Cristina
Fusarium circinatum
Pinus pinea
Pinus radiata
Conifer defense
Disease differential susceptibility
Dual RNA-Seq
Host-pathogen interaction
title_short Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
title_full Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
title_fullStr Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
title_full_unstemmed Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
title_sort Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge
dc.creator.none.fl_str_mv Zamora-Ballesteros, Cristina
Pinto, Gloria
Amaral, Joana
Valledor, Luis
Alves, Artur
Díez, Julio Javier
Martín-García, Jorge
author Zamora-Ballesteros, Cristina
author_facet Zamora-Ballesteros, Cristina
Pinto, Gloria
Amaral, Joana
Valledor, Luis
Alves, Artur
Díez, Julio Javier
Martín-García, Jorge
author_role author
author2 Pinto, Gloria
Amaral, Joana
Valledor, Luis
Alves, Artur
Díez, Julio Javier
Martín-García, Jorge
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Fundação para a Ciência e a Tecnologia (Portugal)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Zamora-Ballesteros, Cristina [0000-0002-9728-5553]
Pinto, Gloria [0000-0001-7735-5131]
Amaral, Joana [0000-0002-3572-0070]
Valledor, Luis [0000-0002-0636-365X]
Alves, Artur [0000-0003-0117-2958]
Díez, Julio Javier [0000-0003-0558-8141]
Martín-García, Jorge [0000-0002-3057-3710]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fusarium circinatum
Pinus pinea
Pinus radiata
Conifer defense
Disease differential susceptibility
Dual RNA-Seq
Host-pathogen interaction
topic Fusarium circinatum
Pinus pinea
Pinus radiata
Conifer defense
Disease differential susceptibility
Dual RNA-Seq
Host-pathogen interaction
description 27 Pág.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372500
https://api.elsevier.com/content/abstract/scopus_id/85105712781
url http://hdl.handle.net/10261/372500
https://api.elsevier.com/content/abstract/scopus_id/85105712781
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110459RB-I00
Instituto Universitario de Investigación en Gestión Forestal Sostenible (iuFOR)
Instituto de Ciencias Forestales (ICIFOR)
publisher
https://doi.org/10.3390/ijms22105231
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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