A novel β-TrCP1/NRF2 interaction inhibitor for effective anti-inflammatory therapy

[Background] Non-communicable chronic diseases are characterized by low-grade inflammation and oxidative stress. Extensive research has identified the transcription factor NRF2 as a potential therapeutic target. Current NRF2 activators, designed to inhibit its repressor KEAP1, often exhibit undesira...

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Detalles Bibliográficos
Autores: García-Yagüe, Ángel Juan, Cañizares-Moscato, Lucía, Encinar, José Antonio, Cazalla, Eduardo, Fernández-Ginés, Raquel, Escoll, Maribel, Rojo, Ana I., Cuadrado, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/413124
Acceso en línea:http://hdl.handle.net/10261/413124
Access Level:acceso abierto
Palabra clave:NRF2
β-TrCP1
Protein–protein interaction-inhibitor
Inflammation
Liver
Descripción
Sumario:[Background] Non-communicable chronic diseases are characterized by low-grade inflammation and oxidative stress. Extensive research has identified the transcription factor NRF2 as a potential therapeutic target. Current NRF2 activators, designed to inhibit its repressor KEAP1, often exhibit undesirable side effects. As an alternative approach, we previously developed PHAR, a protein–protein interaction inhibitor of β-TrCP1/NRF2, which promotes NRF2 activation. Using the same in silico screening platform, we have now identified a novel compound, P10. This small molecule selectively interferes with the β-TrCP1/NRF2 interaction, leading to NRF2 stabilization and transcriptional activation of its target genes in a β-TrCP1-dependent manner, demonstrating promising effects in a liver model of acute inflammation.