Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean

The genus Karyolysus was originally proposed to accommodate blood parasites of lacertid lizards in Western Europe. However, recent phylogenetic analyses suggested an inconclusive taxonomic position of these parasites of the order Adeleorina based on the available genetic information. Inconsistencies...

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Autores: Megía-Palma, Rodrigo M., Martínez, Javier, Fitze, Patrick S., Cuervo, José Javier, Belliure, Josebel, Jiménez-Robles, Octavio, Cabido, Carlos, Martín Rueda, José, Merino, Santiago
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/309030
Acceso en línea:http://hdl.handle.net/10261/309030
Access Level:acceso abierto
Palabra clave:Adeleorina
Co-adaptation
Co-evolution
Karyolysus
Lacertidae
Reptiles
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
title Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
spellingShingle Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
Megía-Palma, Rodrigo M.
Adeleorina
Co-adaptation
Co-evolution
Karyolysus
Lacertidae
Reptiles
title_short Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
title_full Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
title_fullStr Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
title_full_unstemmed Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
title_sort Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean
dc.creator.none.fl_str_mv Megía-Palma, Rodrigo M.
Martínez, Javier
Fitze, Patrick S.
Cuervo, José Javier
Belliure, Josebel
Jiménez-Robles, Octavio
Cabido, Carlos
Martín Rueda, José
Merino, Santiago
author Megía-Palma, Rodrigo M.
author_facet Megía-Palma, Rodrigo M.
Martínez, Javier
Fitze, Patrick S.
Cuervo, José Javier
Belliure, Josebel
Jiménez-Robles, Octavio
Cabido, Carlos
Martín Rueda, José
Merino, Santiago
author_role author
author2 Martínez, Javier
Fitze, Patrick S.
Cuervo, José Javier
Belliure, Josebel
Jiménez-Robles, Octavio
Cabido, Carlos
Martín Rueda, José
Merino, Santiago
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Ministerio de Educación y Ciencia (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Adeleorina
Co-adaptation
Co-evolution
Karyolysus
Lacertidae
Reptiles
topic Adeleorina
Co-adaptation
Co-evolution
Karyolysus
Lacertidae
Reptiles
description The genus Karyolysus was originally proposed to accommodate blood parasites of lacertid lizards in Western Europe. However, recent phylogenetic analyses suggested an inconclusive taxonomic position of these parasites of the order Adeleorina based on the available genetic information. Inconsistencies between molecular phylogeny, morphology, and/or life cycles can reflect lack of enough genetic informa- tion of the target group. We therefore surveyed 28 localities and collected blood samples from 828 lizards of 23 species including lacertids, skinks, and geckoes in the western Mediterranean, North Africa, and Macaronesia, where species of Karyolysus and other adeleorine parasites have been described. We com- bined molecular and microscopic methods to analyze the samples, including those from the host type species and the type locality of Karyolysus bicapsulatus. The phylogenetic relationship of these parasites was analyzed based on the 18S rRNA gene and the co-phylogenetic relationship with their vertebrate hosts was reconstructed. We molecularly detected adeleorine parasites in 37.9% of the blood samples and found 22 new parasite haplotypes. A phylogenetic reconstruction with 132 sequences indicated that 20 of the newly detected haplotypes clustered in a well-supported clade with another 18 sequences that included Karyolysus galloti and Karyolysus lacazei. Morphological evidence also supported that K. bicapsu- latus clustered in this monophyletic clade. These results supported the taxonomic validity of the genus. In addition, we found some parasite haplotypes that infected different lizard host genera with ancient diverging histories, which suggested that Karyolysus is less host-specific than other blood parasites of lizards in the region. A co-phylogenetic analysis supported this interpretation because no significant co-speciation signal was shown between Karyolysus and lizard hosts.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/309030
url http://hdl.handle.net/10261/309030
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MINECO//CGL2014-53523-P
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The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.ijpara.2022.12.006
https://doi.org/10.1016/j.ijpara.2022.12.006
Sí
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western MediterraneanMegía-Palma, Rodrigo M.Martínez, JavierFitze, Patrick S.Cuervo, José JavierBelliure, JosebelJiménez-Robles, OctavioCabido, CarlosMartín Rueda, JoséMerino, SantiagoAdeleorinaCo-adaptationCo-evolutionKaryolysusLacertidaeReptilesThe genus Karyolysus was originally proposed to accommodate blood parasites of lacertid lizards in Western Europe. However, recent phylogenetic analyses suggested an inconclusive taxonomic position of these parasites of the order Adeleorina based on the available genetic information. Inconsistencies between molecular phylogeny, morphology, and/or life cycles can reflect lack of enough genetic informa- tion of the target group. We therefore surveyed 28 localities and collected blood samples from 828 lizards of 23 species including lacertids, skinks, and geckoes in the western Mediterranean, North Africa, and Macaronesia, where species of Karyolysus and other adeleorine parasites have been described. We com- bined molecular and microscopic methods to analyze the samples, including those from the host type species and the type locality of Karyolysus bicapsulatus. The phylogenetic relationship of these parasites was analyzed based on the 18S rRNA gene and the co-phylogenetic relationship with their vertebrate hosts was reconstructed. We molecularly detected adeleorine parasites in 37.9% of the blood samples and found 22 new parasite haplotypes. A phylogenetic reconstruction with 132 sequences indicated that 20 of the newly detected haplotypes clustered in a well-supported clade with another 18 sequences that included Karyolysus galloti and Karyolysus lacazei. Morphological evidence also supported that K. bicapsu- latus clustered in this monophyletic clade. These results supported the taxonomic validity of the genus. In addition, we found some parasite haplotypes that infected different lizard host genera with ancient diverging histories, which suggested that Karyolysus is less host-specific than other blood parasites of lizards in the region. A co-phylogenetic analysis supported this interpretation because no significant co-speciation signal was shown between Karyolysus and lizard hosts.Sampling was funded under the projects CGL2008-00137/BOS granted to JJC by the Spanish Ministry of Science and Innovation and European Regional Development Fund (MICIN-ERDF); PGC2018-097426-B-C21 granted to SM; CGL2012-40026-C02-02 to J. Martínez; CGL2014-53523-P to J. Martín; CGL2008-01522, CGL2012-32459, and CGL2016-76918 to PSF by the Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (MINECO-ERDF). Partial support for sampling in the Guadarrama Mountains (Spain) was provided to OJR by project CGL2011-30393 conceded to I. de la Riva. The Spanish Ministry of Education and Science also funded RMP (BES-2010-038427). RMP holds a postdoctoral contract (CEE-CIND/04084/2017) by BIOPOLIS (Portugal).Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Ministerio de Educación y Ciencia (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/309030reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097426-B-C21info:eu-repo/grantAgreement/MINECO//CGL2014-53523-Pinfo:eu-repo/grantAgreement/MINECO//CGL2012-40026-C02-02info:eu-repo/grantAgreement/MINECO//CGL2014-53523-Pinfo:eu-repo/grantAgreement/MICINN//CGL2008-01522The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.ijpara.2022.12.006https://doi.org/10.1016/j.ijpara.2022.12.006Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3090302026-05-22T06:33:51Z
score 15,81155

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