Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice
In animal models of infammation and in farm animals, dietary inclusion of spray-dried porcine plasma (SDP) reduces mucosal infammation. Here, we study whether these efects could be mediated by changes in the intestinal microbiota and if these changes are similar to those induced by oral antibiotics....
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/171644 |
| Acesso em linha: | https://hdl.handle.net/2445/171644 |
| Access Level: | acceso abierto |
| Palavra-chave: | Suplements nutritius Prebiòtics Microbiota intestinal Nutrició animal Dietary supplements Prebiotics Gastrointestinal microbiome Animal nutrition |
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Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in miceMoretó, Miquel, 1950-Miró Martí, Ma. LluïsaAmat, ConcepcióPolo Pozo, Francisco JavierManichanh, C.Pérez Bosque, AnnaSuplements nutritiusPrebiòticsMicrobiota intestinalNutrició animalDietary supplementsPrebioticsGastrointestinal microbiomeAnimal nutritionIn animal models of infammation and in farm animals, dietary inclusion of spray-dried porcine plasma (SDP) reduces mucosal infammation. Here, we study whether these efects could be mediated by changes in the intestinal microbiota and if these changes are similar to those induced by oral antibiotics. Weaned 21-day-old C57BL/6 mice were divided into 3 groups: the CTL group, fed the control diet; the COL group, administered low doses of neomycin and colistin; and the SDP group, supplemented with 8% SDP. After 14 days, analysis of the fecal microbiome showed that the microbiota profles induced by SDP and the antibiotics were very diferent, thus, SDP has prebiotic rather than antibiotic efects. At the phylum level, SDP stimulated the presence of Firmicutes, considerably increasing the lactobacilli population. It also enhanced the growth of species involved in regulatory T-lymphocyte homeostasis and restoration of the mucosal barrier, as well as species negatively correlated with expression of proinfammatory cytokines. At the mucosal level, expression of toll-like receptors Tlr2, Tlr4 and Tlr9, and mucous-related genes Muc2 and Tf3 with regulatory and barrier stability functions, were increased. SDP also increased expression of Il-10 and Tgf-β, as well as markers of macrophages and dendritic cells eventually promoting an immune-tolerant environment.Nature Publishing Group2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/171644Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-020-59756-zScientific Reports, 2020, vol. 10, num. 1, p. 2926https://doi.org/10.1038/s41598-020-59756-zcc-by (c) Moretó, Miquel, 1950- et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1716442026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| title |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| spellingShingle |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice Moretó, Miquel, 1950- Suplements nutritius Prebiòtics Microbiota intestinal Nutrició animal Dietary supplements Prebiotics Gastrointestinal microbiome Animal nutrition |
| title_short |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| title_full |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| title_fullStr |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| title_full_unstemmed |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| title_sort |
Dietary supplementation with spray-dried porcine plasma has prebiotic efects on gut microbiota in mice |
| dc.creator.none.fl_str_mv |
Moretó, Miquel, 1950- Miró Martí, Ma. Lluïsa Amat, Concepció Polo Pozo, Francisco Javier Manichanh, C. Pérez Bosque, Anna |
| author |
Moretó, Miquel, 1950- |
| author_facet |
Moretó, Miquel, 1950- Miró Martí, Ma. Lluïsa Amat, Concepció Polo Pozo, Francisco Javier Manichanh, C. Pérez Bosque, Anna |
| author_role |
author |
| author2 |
Miró Martí, Ma. Lluïsa Amat, Concepció Polo Pozo, Francisco Javier Manichanh, C. Pérez Bosque, Anna |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Suplements nutritius Prebiòtics Microbiota intestinal Nutrició animal Dietary supplements Prebiotics Gastrointestinal microbiome Animal nutrition |
| topic |
Suplements nutritius Prebiòtics Microbiota intestinal Nutrició animal Dietary supplements Prebiotics Gastrointestinal microbiome Animal nutrition |
| description |
In animal models of infammation and in farm animals, dietary inclusion of spray-dried porcine plasma (SDP) reduces mucosal infammation. Here, we study whether these efects could be mediated by changes in the intestinal microbiota and if these changes are similar to those induced by oral antibiotics. Weaned 21-day-old C57BL/6 mice were divided into 3 groups: the CTL group, fed the control diet; the COL group, administered low doses of neomycin and colistin; and the SDP group, supplemented with 8% SDP. After 14 days, analysis of the fecal microbiome showed that the microbiota profles induced by SDP and the antibiotics were very diferent, thus, SDP has prebiotic rather than antibiotic efects. At the phylum level, SDP stimulated the presence of Firmicutes, considerably increasing the lactobacilli population. It also enhanced the growth of species involved in regulatory T-lymphocyte homeostasis and restoration of the mucosal barrier, as well as species negatively correlated with expression of proinfammatory cytokines. At the mucosal level, expression of toll-like receptors Tlr2, Tlr4 and Tlr9, and mucous-related genes Muc2 and Tf3 with regulatory and barrier stability functions, were increased. SDP also increased expression of Il-10 and Tgf-β, as well as markers of macrophages and dendritic cells eventually promoting an immune-tolerant environment. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020 2020 2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/171644 |
| url |
https://hdl.handle.net/2445/171644 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-59756-z Scientific Reports, 2020, vol. 10, num. 1, p. 2926 https://doi.org/10.1038/s41598-020-59756-z |
| dc.rights.none.fl_str_mv |
cc-by (c) Moretó, Miquel, 1950- et al., 2020 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Moretó, Miquel, 1950- et al., 2020 http://creativecommons.org/licenses/by/3.0/es |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
13 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Nature Publishing Group |
| publisher.none.fl_str_mv |
Nature Publishing Group |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Bioquímica i Fisiologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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15.811543 |