Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content

A hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders th...

Descripción completa

Detalles Bibliográficos
Autores: Talló, Kirian, Bosch Marimon, Manel, Pons, Ramon, Cocera, Mercedes, López, Olga
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/170940
Acceso en línea:https://hdl.handle.net/2445/170940
Access Level:acceso abierto
Palabra clave:Fosfolípids
Microscòpia
Raigs X
Phospholipids
Microscopy
X-rays
id ES_7a464a5cdd4bb96f6e04594fbdcc546c
oai_identifier_str oai:diposit.ub.edu:2445/170940
network_acronym_str ES
network_name_str España
repository_id_str
spelling Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water contentTalló, KirianBosch Marimon, ManelPons, RamonCocera, MercedesLópez, OlgaFosfolípidsMicroscòpiaRaigs XPhospholipidsMicroscopyX-raysA hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders the formation of this type of hydrogel. In this study, a highly hydrated hydrogel (95% water) was formed with hydrogenated phosphatidylcholine and oleic acid. The preparation method involved a freeze-heating cycle of the aqueous lipid mixture, favouring the supramolecular aggregation of these molecules into a microscopic spongy morphology. Confocal fluorescence imaging showed that the microstructure of the hydrogel is made from the aggregation of giant multilamellar vesicles (5-20 μm diameter) while transmission electron microscopy revealed the existence of nanosized unilamellar vesicles (150 nm diameter) coexisting with lipid lamellae. Despite this type of aggregation, X-ray scattering experiments performed on the hydrogel show almost no correlation between lipid membranes. In terms of rheological properties, the material shows a prevalent elastic behaviour and low structural strength, a consequence of non-covalent interactions. With such properties and composition, this structured but easily deformable material might become a useful tool for biomedical applications.Royal Society of Chemistry2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/170940Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1039/C9TB01599JJournal of Materials Chemistry B, 2019, vol. 8, num. 1, p. 161-167https://doi.org/10.1039/C9TB01599Jcc-by-nc (c) Talló et al., 2019http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1709402026-05-27T06:46:51Z
dc.title.none.fl_str_mv Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
spellingShingle Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
Talló, Kirian
Fosfolípids
Microscòpia
Raigs X
Phospholipids
Microscopy
X-rays
title_short Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_full Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_fullStr Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_full_unstemmed Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
title_sort Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content
dc.creator.none.fl_str_mv Talló, Kirian
Bosch Marimon, Manel
Pons, Ramon
Cocera, Mercedes
López, Olga
author Talló, Kirian
author_facet Talló, Kirian
Bosch Marimon, Manel
Pons, Ramon
Cocera, Mercedes
López, Olga
author_role author
author2 Bosch Marimon, Manel
Pons, Ramon
Cocera, Mercedes
López, Olga
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fosfolípids
Microscòpia
Raigs X
Phospholipids
Microscopy
X-rays
topic Fosfolípids
Microscòpia
Raigs X
Phospholipids
Microscopy
X-rays
description A hydrogel formed with phospholipids and fatty acids would be of great interest in the medical field due to the biological relevance that these molecules have in living organisms. However, the tendency of phospholipid mixtures to form vesicular or micellar aggregates at high water content hinders the formation of this type of hydrogel. In this study, a highly hydrated hydrogel (95% water) was formed with hydrogenated phosphatidylcholine and oleic acid. The preparation method involved a freeze-heating cycle of the aqueous lipid mixture, favouring the supramolecular aggregation of these molecules into a microscopic spongy morphology. Confocal fluorescence imaging showed that the microstructure of the hydrogel is made from the aggregation of giant multilamellar vesicles (5-20 μm diameter) while transmission electron microscopy revealed the existence of nanosized unilamellar vesicles (150 nm diameter) coexisting with lipid lamellae. Despite this type of aggregation, X-ray scattering experiments performed on the hydrogel show almost no correlation between lipid membranes. In terms of rheological properties, the material shows a prevalent elastic behaviour and low structural strength, a consequence of non-covalent interactions. With such properties and composition, this structured but easily deformable material might become a useful tool for biomedical applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/170940
url https://hdl.handle.net/2445/170940
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1039/C9TB01599J
Journal of Materials Chemistry B, 2019, vol. 8, num. 1, p. 161-167
https://doi.org/10.1039/C9TB01599J
dc.rights.none.fl_str_mv cc-by-nc (c) Talló et al., 2019
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Talló et al., 2019
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411425931231232
score 15,300719

Similares en LA Referencia