Tailored gelatin-based three-dimensional sysems for bone tissue engineering.

322 p.

Detalles Bibliográficos
Autor: Echave Otaño, María del Carmen
Tipo de recurso: tesis doctoral
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42318
Acceso en línea:http://hdl.handle.net/10810/42318
Access Level:acceso abierto
Palabra clave:cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
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spelling Tailored gelatin-based three-dimensional sysems for bone tissue engineering.Gelatinan oinarritutako hiru dimentsiotako sistema egokituak hezur ehunen ingeniaritzarako.Echave Otaño, María del Carmencultivo celularevaluación de fármacospreparación de fármacoscell cultureevaluation of drugspreparation of drugs322 p.The current Doctoral Thesis is focused on the development of three-dimensional systems for bone tissue engineering purposes. Gelatin was chosen as the main material to manufacture scaffolds capable for serving as both growth factors delivery carrier and cell-therapy support. The main physic-chemical and biological properties of this natural origin polymer, together with the recent advances in gelatin-based approaches for bone tissue engineering are presented in the Introduction part. In the first experimental work, enzymatic crosslinking of gelatin with microbial transglutaminase enzyme has been optimized and in vitro release of VEGF and BMP-2 were characterized. Biological performance of those formulations was evaluated after cell seeding. In the following study, we reinforced the gelatin-based scaffolds with hydroxyapatite and calcium sulfate. In vitro characterization and in vivo bone regeneration capacity was assessed with osteoporotic mice animal model. Reinforced gelatin systems loaded with BMP-2 promoted successful bone repair in the critical sized defects. Finally, we integrated mineralized gelatin composite and bioinspired anisotropic gelatin hydrogel in a single gradual 3D system, in order to emulate the dissimilar composition, architecture and cellular organization found in the musculoskeletal interfaces. The in vitro biological assessment was performed with human adipose-derived stem cell encapsulated within the 3D biphasic hydrogel.Orive Arroyo, GorkaPedraz Muñoz, José Luis2020202020192019info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10810/42318reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésEuskerainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/es/Atribución-NoComercial-CompartirIgual 3.0 España(cc)2019 MARIA DEL CARMEN ECHAVE OTAÑO (cc-by-nc-sa 4.0)oai:addi.ehu.eus:10810/423182026-06-18T09:23:17Z
dc.title.none.fl_str_mv Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
Gelatinan oinarritutako hiru dimentsiotako sistema egokituak hezur ehunen ingeniaritzarako.
title Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
spellingShingle Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
Echave Otaño, María del Carmen
cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
title_short Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
title_full Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
title_fullStr Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
title_full_unstemmed Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
title_sort Tailored gelatin-based three-dimensional sysems for bone tissue engineering.
dc.creator.none.fl_str_mv Echave Otaño, María del Carmen
author Echave Otaño, María del Carmen
author_facet Echave Otaño, María del Carmen
author_role author
dc.contributor.none.fl_str_mv Orive Arroyo, Gorka
Pedraz Muñoz, José Luis
dc.subject.none.fl_str_mv cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
topic cultivo celular
evaluación de fármacos
preparación de fármacos
cell culture
evaluation of drugs
preparation of drugs
description 322 p.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/42318
url http://hdl.handle.net/10810/42318
dc.language.none.fl_str_mv Inglés
Euskera
language_invalid_str_mv Inglés
Euskera
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/3.0/es/
Atribución-NoComercial-CompartirIgual 3.0 España
(cc)2019 MARIA DEL CARMEN ECHAVE OTAÑO (cc-by-nc-sa 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/es/
Atribución-NoComercial-CompartirIgual 3.0 España
(cc)2019 MARIA DEL CARMEN ECHAVE OTAÑO (cc-by-nc-sa 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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