Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain

Infections by the protozoan enteroparasites Giardia duodenalis and Cryptosporidium spp are a major cause of morbidity in children attending day care facilities in developed countries. In this cross-sectional study, we aimed to estimate the occurrence and genotype frequencies of these pathogens in ch...

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Autores: Mateo, Marta, Mateo, María, Montoya, Ana, Bailo-Barroso, Begoña, Saugar, Jose Maria, Aguilera-Rodriguez, Maria, Fuentes Corripio, Isabel, Carmena, David
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/9643
Acceso en línea:http://hdl.handle.net/20.500.12105/9643
Access Level:acceso abierto
Palabra clave:Child, Preschool
Cross-Sectional Studies
Cryptosporidium
DNA, Protozoan
Feces
Female
Giardia lamblia
Giardiasis
Humans
Infant
Male
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Spain
Child Day Care Centers
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spelling Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central SpainMateo, MartaMateo, MaríaMontoya, AnaBailo-Barroso, BegoñaSaugar, Jose MariaAguilera-Rodriguez, MariaFuentes Corripio, IsabelCarmena, DavidChild, PreschoolCross-Sectional StudiesCryptosporidiumDNA, ProtozoanFecesFemaleGiardia lambliaGiardiasisHumansInfantMalePolymorphism, Restriction Fragment LengthSequence Analysis, DNASpainChild Day Care CentersInfections by the protozoan enteroparasites Giardia duodenalis and Cryptosporidium spp are a major cause of morbidity in children attending day care facilities in developed countries. In this cross-sectional study, we aimed to estimate the occurrence and genotype frequencies of these pathogens in children attending day care centers in Majadahonda, Central Spain. To do so, single stool samples were obtained from 90 children and tested for the presence of G duodenalis and Cryptosporidium spp by conventional microscopy and immunochromatography. Positive results by these techniques were subsequently confirmed by immunofluorescence microscopy. G duodenalis-positive samples were subjected to molecular characterization studies by multilocus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of the parasite. G duodenalis assemblages were confirmed by restriction fragment length polymorphism analyses and sequencing. A socioepidemiological questionnaire was used to identify variables potentially associated with giardiasis/cryptosporidiosis in the population of children under investigation. Overall, G duodenalis and Cryptosporidium spp were detected in 15.5% and 3.3% of stool samples, respectively. Giardiasis and cryptosporidiosis were found in 3/3 and 2/3 day care centers, respectively, affecting mainly infants aged 13 to 24 months. A total of 8 G duodenalis isolates were confirmed as subassemblage BIV, all of them belonging to asymptomatic children. Attempts to genotype Cryptosporidium isolates failed. None of the variables considered could be associated with higher risk of infection with giardiasis or cryptosporidiosis. These results clearly indicate that asymptomatic infections with G duodenalis and Cryptosporidium spp are frequent in <3-year-old children in Central Spain.Lippincott Williams & Wilkins (LWW)Instituto de Salud Carlos III20202020-04-2120142014-10-0120142014-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/9643reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/96432026-06-12T12:43:37Z
dc.title.none.fl_str_mv Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
title Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
spellingShingle Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
Mateo, Marta
Child, Preschool
Cross-Sectional Studies
Cryptosporidium
DNA, Protozoan
Feces
Female
Giardia lamblia
Giardiasis
Humans
Infant
Male
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Spain
Child Day Care Centers
title_short Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
title_full Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
title_fullStr Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
title_full_unstemmed Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
title_sort Detection and molecular characterization of Giardia duodenalis in children attending day care centers in Majadahonda, Madrid, Central Spain
dc.creator.none.fl_str_mv Mateo, Marta
Mateo, María
Montoya, Ana
Bailo-Barroso, Begoña
Saugar, Jose Maria
Aguilera-Rodriguez, Maria
Fuentes Corripio, Isabel
Carmena, David
author Mateo, Marta
author_facet Mateo, Marta
Mateo, María
Montoya, Ana
Bailo-Barroso, Begoña
Saugar, Jose Maria
Aguilera-Rodriguez, Maria
Fuentes Corripio, Isabel
Carmena, David
author_role author
author2 Mateo, María
Montoya, Ana
Bailo-Barroso, Begoña
Saugar, Jose Maria
Aguilera-Rodriguez, Maria
Fuentes Corripio, Isabel
Carmena, David
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Child, Preschool
Cross-Sectional Studies
Cryptosporidium
DNA, Protozoan
Feces
Female
Giardia lamblia
Giardiasis
Humans
Infant
Male
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Spain
Child Day Care Centers
topic Child, Preschool
Cross-Sectional Studies
Cryptosporidium
DNA, Protozoan
Feces
Female
Giardia lamblia
Giardiasis
Humans
Infant
Male
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Spain
Child Day Care Centers
description Infections by the protozoan enteroparasites Giardia duodenalis and Cryptosporidium spp are a major cause of morbidity in children attending day care facilities in developed countries. In this cross-sectional study, we aimed to estimate the occurrence and genotype frequencies of these pathogens in children attending day care centers in Majadahonda, Central Spain. To do so, single stool samples were obtained from 90 children and tested for the presence of G duodenalis and Cryptosporidium spp by conventional microscopy and immunochromatography. Positive results by these techniques were subsequently confirmed by immunofluorescence microscopy. G duodenalis-positive samples were subjected to molecular characterization studies by multilocus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of the parasite. G duodenalis assemblages were confirmed by restriction fragment length polymorphism analyses and sequencing. A socioepidemiological questionnaire was used to identify variables potentially associated with giardiasis/cryptosporidiosis in the population of children under investigation. Overall, G duodenalis and Cryptosporidium spp were detected in 15.5% and 3.3% of stool samples, respectively. Giardiasis and cryptosporidiosis were found in 3/3 and 2/3 day care centers, respectively, affecting mainly infants aged 13 to 24 months. A total of 8 G duodenalis isolates were confirmed as subassemblage BIV, all of them belonging to asymptomatic children. Attempts to genotype Cryptosporidium isolates failed. None of the variables considered could be associated with higher risk of infection with giardiasis or cryptosporidiosis. These results clearly indicate that asymptomatic infections with G duodenalis and Cryptosporidium spp are frequent in <3-year-old children in Central Spain.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-10-01
2014
2014-10-01
2020
2020-04-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/9643
url http://hdl.handle.net/20.500.12105/9643
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins (LWW)
publisher.none.fl_str_mv Lippincott Williams & Wilkins (LWW)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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