Supporting Information: Aspergillus niger ochratoxinase is a highly specific, metal-dependent amidohydrolase suitable for OTA biodetoxification in food and feed
Scheme of the hydrolysis of the amide bond of OTA, SDS-PAGE analysis of recombinant AnOTA from A. niger CBS 513.88, structural comparison of the AnOTA subunit with those from its close structural homologues, structures of the substrates used in our study classified into five families and also those...
| Autores: | , , , , |
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| Formato: | conjunto de datos |
| Fecha de publicación: | 2024 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/381760 |
| Acesso em linha: | http://hdl.handle.net/10261/381760 |
| Access Level: | acceso abierto |
| Resumo: | Scheme of the hydrolysis of the amide bond of OTA, SDS-PAGE analysis of recombinant AnOTA from A. niger CBS 513.88, structural comparison of the AnOTA subunit with those from its close structural homologues, structures of the substrates used in our study classified into five families and also those of OTA and 4MF, docking of OTA into the cryoEM 3D structure of the AnOTA homologue enzyme ADH3 from S. acidaminiphila (SaOTA) and comparison with the experimentally determined structure of the mycotoxin, and SDS-PAGE analysis of plant-based beverages containing OTA (5 μM) incubated with AnOTA at 37 °C for 18 h. |
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