Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2
[EN]The four receptor tyrosine kinases of the ErbB family play essential roles in several physiological processes and have also been implicated in tumor generation and/or progression. Activation of ErbB1/EGFR is mainly triggered by epidermal growth factor (EGF) and other related ligands, while activ...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2002 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/167831 |
| Acesso em linha: | http://hdl.handle.net/10366/167831 |
| Access Level: | acceso abierto |
| Palavra-chave: | Erk5, Breast cancer, signaling, Neuregulin Breast Neoplasms Neuregulins Humans Cell Division Antineoplastic Agents Microscopy Enzyme Inhibitors Mitogen-Activated Protein Kinases Phthalimides Phosphorylation MAP Kinase Signaling System Nitriles Butadienes Mitogen-Activated Protein Kinase 7 Recombinant Fusion Proteins 2302 Bioquímica proteínas de fusión recombinantes humanos inhibidores enzimáticos proteína cinasa activada por mitógenos 7 nitrilos proteína cinasas activadas por mitógenos división celular microscopía sistema de señalización de las MAP cinasas antineoplásicos butadienos neurregulinas neoplasias de la mama fosforilación ftalimidas |
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Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2Esparís Ogando, AzucenaDíaz Rodríguez, María ElenaMontero, Juan CarlosYuste, LauraCrespo, PieroPandiella Alonso, AtanasioErk5, Breast cancer, signaling, NeuregulinBreast NeoplasmsNeuregulinsHumansCell DivisionAntineoplastic AgentsMicroscopyEnzyme InhibitorsMitogen-Activated Protein KinasesPhthalimidesPhosphorylationMAP Kinase Signaling SystemNitrilesButadienesMitogen-Activated Protein Kinase 7Recombinant Fusion Proteins2302 Bioquímicaproteínas de fusión recombinanteshumanosinhibidores enzimáticosproteína cinasa activada por mitógenos 7nitrilosproteína cinasas activadas por mitógenosdivisión celularmicroscopíasistema de señalización de las MAP cinasasantineoplásicosbutadienosneurregulinasneoplasias de la mamafosforilaciónftalimidas[EN]The four receptor tyrosine kinases of the ErbB family play essential roles in several physiological processes and have also been implicated in tumor generation and/or progression. Activation of ErbB1/EGFR is mainly triggered by epidermal growth factor (EGF) and other related ligands, while activation of ErbB2, ErbB3, and ErbB4 receptors occurs by binding to another set of EGF-like ligands termed neuregulins (NRGs). Here we show that the Erk5 mitogen-activated protein kinase (MAPK) pathway participates in NRG signal transduction. In MCF7 cells, NRG activated Erk5 in a time- and dose-dependent fashion. The action of NRG on Erk5 was dependent on the kinase activity of ErbB receptors but was independent of Ras. Expression in MCF7 cells of a dominant negative form of Erk5 resulted in a significant decrease in NRG-induced proliferation of MCF7 cells. Analysis of Erk5 in several human tumor cell lines indicated that a constitutively active form of this kinase was present in the BT474 and SKBR3 cell lines, which also expressed activated forms of ErbB2, ErbB3, and ErbB4. Treatments aimed at decreasing the activity of these receptors caused Erk5 inactivation, indicating that the active form of Erk5 present in BT474 and SKBR3 cells was due to a persistent positive stimulus originating at the ErbB receptors. In BT474 cells expression of the dominant negative form of Erk5 resulted in reduced proliferation, indicating that in these cells Erk5 was also involved in the control of proliferation. Taken together, these results suggest that Erk5 may play a role in the regulation of cell proliferation by NRG receptors and indicate that constitutively active NRG receptors may induce proliferative responses in cancer cells through this MAPK pathway.Taylor & Francis202520252002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/167831reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésDGES PM97-0061Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1678312026-06-07T06:28:51Z |
| dc.title.none.fl_str_mv |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| title |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| spellingShingle |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 Esparís Ogando, Azucena Erk5, Breast cancer, signaling, Neuregulin Breast Neoplasms Neuregulins Humans Cell Division Antineoplastic Agents Microscopy Enzyme Inhibitors Mitogen-Activated Protein Kinases Phthalimides Phosphorylation MAP Kinase Signaling System Nitriles Butadienes Mitogen-Activated Protein Kinase 7 Recombinant Fusion Proteins 2302 Bioquímica proteínas de fusión recombinantes humanos inhibidores enzimáticos proteína cinasa activada por mitógenos 7 nitrilos proteína cinasas activadas por mitógenos división celular microscopía sistema de señalización de las MAP cinasas antineoplásicos butadienos neurregulinas neoplasias de la mama fosforilación ftalimidas |
| title_short |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| title_full |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| title_fullStr |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| title_full_unstemmed |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| title_sort |
Erk5 participates in neuregulin signal transduction and is constitutively active in breast cancer cells overexpressing ErbB2 |
| dc.creator.none.fl_str_mv |
Esparís Ogando, Azucena Díaz Rodríguez, María Elena Montero, Juan Carlos Yuste, Laura Crespo, Piero Pandiella Alonso, Atanasio |
| author |
Esparís Ogando, Azucena |
| author_facet |
Esparís Ogando, Azucena Díaz Rodríguez, María Elena Montero, Juan Carlos Yuste, Laura Crespo, Piero Pandiella Alonso, Atanasio |
| author_role |
author |
| author2 |
Díaz Rodríguez, María Elena Montero, Juan Carlos Yuste, Laura Crespo, Piero Pandiella Alonso, Atanasio |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Erk5, Breast cancer, signaling, Neuregulin Breast Neoplasms Neuregulins Humans Cell Division Antineoplastic Agents Microscopy Enzyme Inhibitors Mitogen-Activated Protein Kinases Phthalimides Phosphorylation MAP Kinase Signaling System Nitriles Butadienes Mitogen-Activated Protein Kinase 7 Recombinant Fusion Proteins 2302 Bioquímica proteínas de fusión recombinantes humanos inhibidores enzimáticos proteína cinasa activada por mitógenos 7 nitrilos proteína cinasas activadas por mitógenos división celular microscopía sistema de señalización de las MAP cinasas antineoplásicos butadienos neurregulinas neoplasias de la mama fosforilación ftalimidas |
| topic |
Erk5, Breast cancer, signaling, Neuregulin Breast Neoplasms Neuregulins Humans Cell Division Antineoplastic Agents Microscopy Enzyme Inhibitors Mitogen-Activated Protein Kinases Phthalimides Phosphorylation MAP Kinase Signaling System Nitriles Butadienes Mitogen-Activated Protein Kinase 7 Recombinant Fusion Proteins 2302 Bioquímica proteínas de fusión recombinantes humanos inhibidores enzimáticos proteína cinasa activada por mitógenos 7 nitrilos proteína cinasas activadas por mitógenos división celular microscopía sistema de señalización de las MAP cinasas antineoplásicos butadienos neurregulinas neoplasias de la mama fosforilación ftalimidas |
| description |
[EN]The four receptor tyrosine kinases of the ErbB family play essential roles in several physiological processes and have also been implicated in tumor generation and/or progression. Activation of ErbB1/EGFR is mainly triggered by epidermal growth factor (EGF) and other related ligands, while activation of ErbB2, ErbB3, and ErbB4 receptors occurs by binding to another set of EGF-like ligands termed neuregulins (NRGs). Here we show that the Erk5 mitogen-activated protein kinase (MAPK) pathway participates in NRG signal transduction. In MCF7 cells, NRG activated Erk5 in a time- and dose-dependent fashion. The action of NRG on Erk5 was dependent on the kinase activity of ErbB receptors but was independent of Ras. Expression in MCF7 cells of a dominant negative form of Erk5 resulted in a significant decrease in NRG-induced proliferation of MCF7 cells. Analysis of Erk5 in several human tumor cell lines indicated that a constitutively active form of this kinase was present in the BT474 and SKBR3 cell lines, which also expressed activated forms of ErbB2, ErbB3, and ErbB4. Treatments aimed at decreasing the activity of these receptors caused Erk5 inactivation, indicating that the active form of Erk5 present in BT474 and SKBR3 cells was due to a persistent positive stimulus originating at the ErbB receptors. In BT474 cells expression of the dominant negative form of Erk5 resulted in reduced proliferation, indicating that in these cells Erk5 was also involved in the control of proliferation. Taken together, these results suggest that Erk5 may play a role in the regulation of cell proliferation by NRG receptors and indicate that constitutively active NRG receptors may induce proliferative responses in cancer cells through this MAPK pathway. |
| publishDate |
2002 |
| dc.date.none.fl_str_mv |
2002 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10366/167831 |
| url |
http://hdl.handle.net/10366/167831 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
DGES PM97-0061 |
| dc.rights.none.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Taylor & Francis |
| publisher.none.fl_str_mv |
Taylor & Francis |
| dc.source.none.fl_str_mv |
reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL) |
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Universidad de Salamanca (USAL) |
| reponame_str |
GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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15,81155 |