Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells

Small ruminant lentiviruses (SRLVs) are widely spread in the ovine and caprine populations, causing an incurable disease affecting animal health and production. Vaccine development is hindered owing to the high genetic heterogeneity of lentiviruses and the selection of T-cell and antibody escape mut...

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Autores: Pablo, Lorena de, Echeverría, Irache, Rius-Rocabert, Sergio, Luján, Lluís, Garcin, Dominique, Andrés, Damián F. de, Nistal-Villán, Estanislao, Reina, Ramsés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215624
Acceso en línea:http://hdl.handle.net/10261/215624
Access Level:acceso abierto
Palabra clave:Small ruminant lentivirus
Sendai virus
Innate immunity
Interferon
APOBEC3
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spelling Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine CellsPablo, Lorena deEcheverría, IracheRius-Rocabert, SergioLuján, LluísGarcin, DominiqueAndrés, Damián F. deNistal-Villán, EstanislaoReina, RamsésSmall ruminant lentivirusSendai virusInnate immunityInterferonAPOBEC3Small ruminant lentiviruses (SRLVs) are widely spread in the ovine and caprine populations, causing an incurable disease affecting animal health and production. Vaccine development is hindered owing to the high genetic heterogeneity of lentiviruses and the selection of T-cell and antibody escape mutants, requiring antigen delivery optimization. Sendai virus (SeV) is a respiratory paramyxovirus in mice that has been recognized as a potent inducer of innate immune responses in several species, including mouse and human. The aim of this study was to stimulate an innate antiviral response in ovine cells and evaluate the potential inhibitory effect upon small ruminant lentivirus (SRLV) infections. Ovine alveolar macrophages (AMs), blood-derived macrophages (BDMs), and skin fibroblasts (OSFs) were stimulated through infection with SeV encoding green fluorescent protein (GFP). SeV efficiently infected ovine cells, inducing an antiviral state in AM from SRLV naturally-infected animals, as well as in in vitro SRLV-infected BDM and OSF from non-infected animals. Supernatants from SeV-infected AM induced an antiviral state when transferred to fresh cells challenged with SRLV. Similar to SRLV, infectivity of an HIV-1-GFP lentiviral vector was also restricted in ovine cells infected with SeV. In myeloid cells, an M1-like proinflammatory polarization was observed together with an APOBEC3Z1 induction, among other lentiviral restriction factors. Our observations may boost new approximations in ameliorating the SRLV burden by stimulation of the innate immune response using SeV-based vaccine vectors.This research was funded by Spanish Ministry of Science, Innovation, and Universities, grant number RTI2018-096172-B-C31; Consejo Superior de Investigaciones Científicas, i-Coop and EMHE Program; and by Government of Navarra (CONECTIM) and by Project NIETO-CM B2017/BMD-3731 to E.N.-V. “The APC was funded by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).” L.d.P.-M. and I.E. were funded by Universidad Pública de Navarra. S.R.-R. was funded by an FPI fellowship granted by Universidad San Pablo CEU. R.R. was supported by the Spanish Ministry of Science and Innovation “Ramón y Cajal” contract.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad de NavarraCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Universidad de San Pablo-CEUConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215624reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C31RTI2018-096172-B-C31/AEI/10.13039/501100011033B2017/BMD-3731/NIETO-CMhttps://doi.org/10.3390/vaccines8020206Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2156242026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
title Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
spellingShingle Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
Pablo, Lorena de
Small ruminant lentivirus
Sendai virus
Innate immunity
Interferon
APOBEC3
title_short Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
title_full Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
title_fullStr Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
title_full_unstemmed Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
title_sort Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
dc.creator.none.fl_str_mv Pablo, Lorena de
Echeverría, Irache
Rius-Rocabert, Sergio
Luján, Lluís
Garcin, Dominique
Andrés, Damián F. de
Nistal-Villán, Estanislao
Reina, Ramsés
author Pablo, Lorena de
author_facet Pablo, Lorena de
Echeverría, Irache
Rius-Rocabert, Sergio
Luján, Lluís
Garcin, Dominique
Andrés, Damián F. de
Nistal-Villán, Estanislao
Reina, Ramsés
author_role author
author2 Echeverría, Irache
Rius-Rocabert, Sergio
Luján, Lluís
Garcin, Dominique
Andrés, Damián F. de
Nistal-Villán, Estanislao
Reina, Ramsés
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad de Navarra
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Universidad de San Pablo-CEU
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Small ruminant lentivirus
Sendai virus
Innate immunity
Interferon
APOBEC3
topic Small ruminant lentivirus
Sendai virus
Innate immunity
Interferon
APOBEC3
description Small ruminant lentiviruses (SRLVs) are widely spread in the ovine and caprine populations, causing an incurable disease affecting animal health and production. Vaccine development is hindered owing to the high genetic heterogeneity of lentiviruses and the selection of T-cell and antibody escape mutants, requiring antigen delivery optimization. Sendai virus (SeV) is a respiratory paramyxovirus in mice that has been recognized as a potent inducer of innate immune responses in several species, including mouse and human. The aim of this study was to stimulate an innate antiviral response in ovine cells and evaluate the potential inhibitory effect upon small ruminant lentivirus (SRLV) infections. Ovine alveolar macrophages (AMs), blood-derived macrophages (BDMs), and skin fibroblasts (OSFs) were stimulated through infection with SeV encoding green fluorescent protein (GFP). SeV efficiently infected ovine cells, inducing an antiviral state in AM from SRLV naturally-infected animals, as well as in in vitro SRLV-infected BDM and OSF from non-infected animals. Supernatants from SeV-infected AM induced an antiviral state when transferred to fresh cells challenged with SRLV. Similar to SRLV, infectivity of an HIV-1-GFP lentiviral vector was also restricted in ovine cells infected with SeV. In myeloid cells, an M1-like proinflammatory polarization was observed together with an APOBEC3Z1 induction, among other lentiviral restriction factors. Our observations may boost new approximations in ameliorating the SRLV burden by stimulation of the innate immune response using SeV-based vaccine vectors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215624
url http://hdl.handle.net/10261/215624
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C31
RTI2018-096172-B-C31/AEI/10.13039/501100011033
B2017/BMD-3731/NIETO-CM
https://doi.org/10.3390/vaccines8020206
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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