Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice

[Background] Rice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Salt...

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Autores: Marè, Caterina, Zampieri, Elisa, Cavallaro, Viviana, Frouin, Julien, Grenier, Cécile, Courtois, Brigitte, Brottier, Laurent, Tacconi, Gianni, Finocchiaro, Franca, Serrat, Xavier, Nogués, Salvador, Bundó, Mireia, San Segundo, Blanca, Negrini, Noemi, Pesenti, Michele, Sacchi, Gian Attilio, Gavina, Giacomo, Bovina, Riccardo, Monaco, Stefano, Tondelli, Alessandro, Cattivelli, Luigi, Valè, Giampiero
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/335464
Acesso em linha:http://hdl.handle.net/10261/335464
https://api.elsevier.com/content/abstract/scopus_id/85146272461
Access Level:acceso abierto
Palavra-chave:Background selection
Breeding
KASP markers
MABC
Recovery percentage
Rice
Salt tolerance
Saltol
id ES_797c021e000b36bfec010b5a48b8f84b
oai_identifier_str oai:digital.csic.es:10261/335464
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
title Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
spellingShingle Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
Marè, Caterina
Background selection
Breeding
KASP markers
MABC
Recovery percentage
Rice
Salt tolerance
Saltol
title_short Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
title_full Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
title_fullStr Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
title_full_unstemmed Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
title_sort Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice
dc.creator.none.fl_str_mv Marè, Caterina
Zampieri, Elisa
Cavallaro, Viviana
Frouin, Julien
Grenier, Cécile
Courtois, Brigitte
Brottier, Laurent
Tacconi, Gianni
Finocchiaro, Franca
Serrat, Xavier
Nogués, Salvador
Bundó, Mireia
San Segundo, Blanca
Negrini, Noemi
Pesenti, Michele
Sacchi, Gian Attilio
Gavina, Giacomo
Bovina, Riccardo
Monaco, Stefano
Tondelli, Alessandro
Cattivelli, Luigi
Valè, Giampiero
author Marè, Caterina
author_facet Marè, Caterina
Zampieri, Elisa
Cavallaro, Viviana
Frouin, Julien
Grenier, Cécile
Courtois, Brigitte
Brottier, Laurent
Tacconi, Gianni
Finocchiaro, Franca
Serrat, Xavier
Nogués, Salvador
Bundó, Mireia
San Segundo, Blanca
Negrini, Noemi
Pesenti, Michele
Sacchi, Gian Attilio
Gavina, Giacomo
Bovina, Riccardo
Monaco, Stefano
Tondelli, Alessandro
Cattivelli, Luigi
Valè, Giampiero
author_role author
author2 Zampieri, Elisa
Cavallaro, Viviana
Frouin, Julien
Grenier, Cécile
Courtois, Brigitte
Brottier, Laurent
Tacconi, Gianni
Finocchiaro, Franca
Serrat, Xavier
Nogués, Salvador
Bundó, Mireia
San Segundo, Blanca
Negrini, Noemi
Pesenti, Michele
Sacchi, Gian Attilio
Gavina, Giacomo
Bovina, Riccardo
Monaco, Stefano
Tondelli, Alessandro
Cattivelli, Luigi
Valè, Giampiero
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Background selection
Breeding
KASP markers
MABC
Recovery percentage
Rice
Salt tolerance
Saltol
topic Background selection
Breeding
KASP markers
MABC
Recovery percentage
Rice
Salt tolerance
Saltol
description [Background] Rice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Saltol major QTL confers capacity to young rice plants growing under salt condition by maintaining a low Na+/K+ molar ratio in the shoots.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/335464
https://api.elsevier.com/content/abstract/scopus_id/85146272461
url http://hdl.handle.net/10261/335464
https://api.elsevier.com/content/abstract/scopus_id/85146272461
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/678168
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s12284-023-00619-2
Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 2 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561018
Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 3 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561021
Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 5 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561027
Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 4 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561024
https://doi.org/10.1186/s12284-023-00619-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica RiceMarè, CaterinaZampieri, ElisaCavallaro, VivianaFrouin, JulienGrenier, CécileCourtois, BrigitteBrottier, LaurentTacconi, GianniFinocchiaro, FrancaSerrat, XavierNogués, SalvadorBundó, MireiaSan Segundo, BlancaNegrini, NoemiPesenti, MicheleSacchi, Gian AttilioGavina, GiacomoBovina, RiccardoMonaco, StefanoTondelli, AlessandroCattivelli, LuigiValè, GiampieroBackground selectionBreedingKASP markersMABCRecovery percentageRiceSalt toleranceSaltol[Background] Rice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Saltol major QTL confers capacity to young rice plants growing under salt condition by maintaining a low Na+/K+ molar ratio in the shoots.[Results] Marker-assisted backcross (MABC) procedure was adopted to transfer Saltol locus conferring salt tolerance at seedling stage from donor indica IR64-Saltol to two temperate japonica varieties, Vialone Nano and Onice. Forward and background selections were accomplished using polymorphic KASP markers and a final evaluation of genetic background recovery of the selected lines was conducted using 15,580 SNP markers obtained from Genotyping by Sequencing. Three MABC generations followed by two selfing, allowed the identification of introgression lines achieving a recovery of the recurrent parent (RP) genome up to 100% (based on KASP markers) or 98.97% (based on GBS). Lines with highest RP genome recovery (RPGR) were evaluated for agronomical-phenological traits in field under non-salinized conditions. VN1, VN4, O1 lines were selected considering the agronomic evaluations and the RPGR% results as the most interesting for commercial exploitation. A physiological characterization was conducted by evaluating salt tolerance under hydroponic conditions. The selected lines showed lower standard evaluation system (SES) scores: 62% of VN4, and 57% of O1 plants reaching SES 3 or SES 5 respectively, while only 40% of Vialone Nano and 25% of Onice plants recorded scores from 3 to 5, respectively. VN1, VN4 and O1 showed a reduced electrolyte leakage values, and limited negative effects on relative water content and shoot/root fresh weight ratio.[Conclusion] The Saltol locus was successfully transferred to two elite varieties by MABC in a time frame of three years. The application of background selection until BC3F3 allowed the selection of lines with a RPGR up to 98.97%. Physiological evaluations for the selected lines indicate an improved salinity tolerance at seedling stage. The results supported the effectiveness of the Saltol locus in temperate japonica and of the MABC procedure for recovering of the RP favorable traits.We acknowledge the support of NEURICE project (New commercial European RICE (Oryza sativa) harbouring salt tolerance alleles to protect the rice sector against climate change and apple snail (Pomacea insularum) invasion, Grant Agreement n. 678168).Peer reviewedSpringerEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/335464https://api.elsevier.com/content/abstract/scopus_id/85146272461reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/678168The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s12284-023-00619-2Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 2 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561018Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 3 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561021Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 5 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561027Marè, Caterina; Zampieri, Elisa; Cavallaro, Viviana; Frouin, Julien; Grenier, Cécile; Courtois, Brigitte; Brottier, Laurent; Tacconi, Gianni; Finocchiaro, Franca; Serrat, Xavier; Nogués, Salvador; Bundó, Mireia; San Segundo, Blanca; Negrini, Noemi; Pesenti, Michele; Sacchi, Gian Attilio; Gavina, Giacomo; Bovina, Riccardo; Monaco, Stefano; Tondelli, Alessandro; Cattivelli, Luigi; Valè, Giampiero; 2024; Additional file 4 of Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice [Dataset]; Figshare; https://doi.org/10.6084/m9.figshare.26561024https://doi.org/10.1186/s12284-023-00619-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3354642026-05-22T06:33:51Z
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