Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of...
| Autores: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:185445 |
| Acceso en línea: | https://ddd.uab.cat/record/185445 https://dx.doi.org/urn:doi:10.1002/eji.201545890 |
| Access Level: | acceso abierto |
| Palabra clave: | Cytotoxic T cells Human immunodeficiency virus type I Human leukocyte antigen Immunopeptidome Mass spectrometry |
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Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cellsTernette, NicolaYang, HongbingPartridge, ThomasLlano Montero, Anuska|||0000-0002-0133-9888Cedeño, Samandhy|||0000-0001-8312-0208Fischer, RomanCharles, Philip D.Dudek, Nadine L.Mothe, Beatriz|||0000-0001-9975-407XCrespo Casal, Manuel|||0000-0001-9016-0515Fischer, William M.Korber, Bette T. M.Nielsen, MortenBorrow, PersephonePurcell, Anthony W.Brander, Christian|||0000-0002-0548-5778Dorrell, LucyKessler, Benedikt M.Hanke, Tomáš|||0000-0002-6076-9546Cytotoxic T cellsHuman immunodeficiency virus type IHuman leukocyte antigenImmunopeptidomeMass spectrometryRecognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4 + T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.Universitat Autònoma de Barcelona 22015-01-0120152015-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/185445https://dx.doi.org/urn:doi:10.1002/eji.201545890reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 MTM2008-06747-C02-00Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CM08-00020open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1854452026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| title |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| spellingShingle |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells Ternette, Nicola Cytotoxic T cells Human immunodeficiency virus type I Human leukocyte antigen Immunopeptidome Mass spectrometry |
| title_short |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| title_full |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| title_fullStr |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| title_full_unstemmed |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| title_sort |
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells |
| dc.creator.none.fl_str_mv |
Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano Montero, Anuska|||0000-0002-0133-9888 Cedeño, Samandhy|||0000-0001-8312-0208 Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz|||0000-0001-9975-407X Crespo Casal, Manuel|||0000-0001-9016-0515 Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian|||0000-0002-0548-5778 Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš|||0000-0002-6076-9546 |
| author |
Ternette, Nicola |
| author_facet |
Ternette, Nicola Yang, Hongbing Partridge, Thomas Llano Montero, Anuska|||0000-0002-0133-9888 Cedeño, Samandhy|||0000-0001-8312-0208 Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz|||0000-0001-9975-407X Crespo Casal, Manuel|||0000-0001-9016-0515 Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian|||0000-0002-0548-5778 Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš|||0000-0002-6076-9546 |
| author_role |
author |
| author2 |
Yang, Hongbing Partridge, Thomas Llano Montero, Anuska|||0000-0002-0133-9888 Cedeño, Samandhy|||0000-0001-8312-0208 Fischer, Roman Charles, Philip D. Dudek, Nadine L. Mothe, Beatriz|||0000-0001-9975-407X Crespo Casal, Manuel|||0000-0001-9016-0515 Fischer, William M. Korber, Bette T. M. Nielsen, Morten Borrow, Persephone Purcell, Anthony W. Brander, Christian|||0000-0002-0548-5778 Dorrell, Lucy Kessler, Benedikt M. Hanke, Tomáš|||0000-0002-6076-9546 |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Cytotoxic T cells Human immunodeficiency virus type I Human leukocyte antigen Immunopeptidome Mass spectrometry |
| topic |
Cytotoxic T cells Human immunodeficiency virus type I Human leukocyte antigen Immunopeptidome Mass spectrometry |
| description |
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4 + T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2 2015-01-01 2015 2015-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/185445 https://dx.doi.org/urn:doi:10.1002/eji.201545890 |
| url |
https://ddd.uab.cat/record/185445 https://dx.doi.org/urn:doi:10.1002/eji.201545890 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 MTM2008-06747-C02-00 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CM08-00020 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/3.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/3.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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